You’ve made a 25 mM stock of colchicine (a tubulin inhibitor; dissolved in distilled water), which you’d like to use to study the effect of tubulin on phagocytosis in T. pyriformis. Based on published research articles that you’ve read, you’d like to preincubate T. pyriformis with colchicine at a final, effective concentration of 75 µM. Assuming you need 40 µL of the cell+colchicine mixture for your experiment, please explain how you can use your 25 mM solution of colchicine, T. pyriformis cells, and distilled water to create your cell+colchicine mixture
You’ve made a 25 mM stock of colchicine (a tubulin inhibitor; dissolved in distilled water), which you’d like to use to study the effect of tubulin on phagocytosis in T. pyriformis. Based on published research articles that you’ve read, you’d like to preincubate T. pyriformis with colchicine at a final, effective concentration of 75 µM. Assuming you need 40 µL of the cell+colchicine mixture for your experiment, please explain how you can use your 25 mM solution of colchicine, T. pyriformis cells, and distilled water to create your cell+colchicine mixture
Introduction to General, Organic and Biochemistry
11th Edition
ISBN:9781285869759
Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres
Chapter31: Immunochemistry
Section: Chapter Questions
Problem 31.72P
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You’ve made a 25 mM stock of colchicine (a tubulin inhibitor; dissolved in distilled water), which you’d like to use to study the effect of tubulin on phagocytosis in T. pyriformis. Based on published research articles that you’ve read, you’d like to preincubate T. pyriformis with colchicine at a final, effective concentration of 75 µM. Assuming you need 40 µL of the cell+colchicine mixture for your experiment, please explain how you can use your 25 mM solution of colchicine, T. pyriformis cells, and distilled water to create your cell+colchicine mixture.
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