ATP NAD+ FADH2 Coenzyme A ATP synthase oxidizing agent in metabolism enzyme "motor" located on the inner membrane of mitochondria works with CoQ10 in oxidative phosphorylation shuttles electrons in the e- transport chain provides energy to drive an endergonic reaction forward shuttles two-carbon units (acetyl groups) reducing agent in metabolism turns pyruvate to lactate in anaerobic conditions
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- In oxidative phosphorylation.... 1. Succinate contributes 2e- to Complex II and 2H+ to the mitochondrial proton gradient. 2. NADH in the matrix passes 2 e- to coenzyme Q via Complex I. 3. Complexes I, II, III, and IV each contribute to the matrix proton gradient. 4. O2 stabilizes the catalytically active conformation of Complex V. 5. Reversible protonation of c subunits leads to rotation of the Complex V gamma subunit. 6. Each β subunit can bind ATP tightly under the right conditions. 7. For every 3 protons that pass across the inner mitochondrial membrane, 1 ATP is produced. Choose all options that are true.In oxidative phosphorylation (mark all that are true)... Choice 1 of 7:Succinate contributes 2e- to Complex II and 2H+ to the mitochondrial proton gradient. Choice 2 of 7:NADH in the matrix passes 2 e- to coenzyme Q via Complex I. Choice 3 of 7:Complexes I, II, III, and IV each contribute to the matrix proton gradient. Choice 4 of 7:O2 stabilizes the catalytically active conformation of Complex V. Choice 5 of 7:Reversible protonation of c subunits leads to rotation of the Complex V gamma subunit. Choice 6 of 7:Each β subunit can bind ATP tightly under the right conditions. Choice 7 of 7:For every 3 protons that pass across the inner mitochondrial membrane, 1 ATP is produced. Below are screenshots of the answer from two different sources (for your consideration)Given that malonate inhibits succinate dehydrogenase which of the following statements is TRUE? a. FADH2 results in only 4 H+ being pumped out of the mitochondrial matrix. b. All electron transport is inhibited. c. Transfer of electrons from NADH to Coenzyme Q (ubiquinone) is inhibited. d. Transfer of electrons from FADH2 to Coenzyme Q (ubiquinone) is inhibited. e. NADH results in only 4 H+ being pumped out of the mitochondrial matrix. Clear my choice
- For the Complex III in the electron transport chain: Complex III step 1: UQH2 is oxidized in a 2 electron process. Cytochrome c is reduced and UQ is reduced to UQH in two 1 electron processes. Complex III step 2: UQH2 is oxidized in a 2 electron process. Cytochrome c is reduced and UQH is reduced to UQH2 in two 1 electron processes. The necessary standard reduction potentials are: UQ + 2H+ + 2e- UQH2 E° = 0.06 V cyt c (Fe3+) + e- cyt c (Fe2+) E° = 0.254 V UQ + H+ + e- UQH. E° = 0.03 V UQH. + H+ + e- UQH2 E° = 0.19 V Calculate the total redox potential of the complex. Now calculate how many moles of protons can be translocated across the inner mitochondrial membrane if translocation of 1 mole requires 23 kJ. Calculate the free energy available for proton translocation assuming a 2electron process for each complex.Assuming that the glycerol 3-phosphate shuttle is operative, a complete oxidation of five molecules of glucose (by a eukaryotic cell) into carbon dioxide and water would produce approximately _________ ATP molecules via oxidative phosphorylation only. (Consider NADH = 2.5 ATP and FADH2 = 1.5 ATP)Concerning the citric acid cycle (TCA cycle), which of the following statements is false? The TCA cycle generates electron carriers Oxidation of pyruvate in the matrix of the mitochondria produces sixteen total electrons Substrate level phosphorylation in the TCA cycle produces 2 GTP per the oxidation of a single glucose molecule The fate of the carbonyl carbon of acetyl CoAin the TCA cycle is to be lost as CO2 in the second turn Oxygen is required to regenerate electron acceptors
- GAP + Pi + NAD+ ⟹1,3-BPG + NADH (Reaction 1) 1,3-BPG + ADP ⟹ 3-PG + ATP (Reaction 2) Which of the following statements concerning the information above is true? Select all that apply. A.Reaction 2 is an example of substrate level phosphorylation B.These reactions are written in a direction that would indicate gluconeogenesis is occurring in the liver C.The linking of Reaction 1 and Reaction 2 by the intermediate 1,3-BPG is an example of coupling of reactions D.Reaction 1 shows a redox reaction where the carbon skeleton is oxidized to generate electrons for a soluble electron carrier E.These reactions are irreversible under cellular conditionsConsider a 24:1 △cis-9 fatty acid in the mitochondrion. For each fatty acid given, determine the following. 1. Gross ATP from b-oxidation cycles 2. Gross ATP from acetyl CoA produced 3. Gross ATP from conversion of propionyl CoA (if applicable) 4. Total number of ATP deducted 5. Total net ATPWhich of the following dehydrogenases delivers electrons to directly to UQ in the electron transport pathway from the intermembrane space side of the inner mitochondrial membrane? a. succinate dehydrogenase b. acyl CoA dehydrogenase c. creatine kinase dehydrogenase d. glycerol-3-phosphate dehydrogenase
- Consider a brain cell with non-functional Complex II of the electron transport chain (Assume that the deficiency is isolated and will not influence the function of other respiration components) In these cells/tissues, determine the following from the catabolism of the 2.5 moles of the disaccharide lactose (will be hydrolyzed first to yield glucose and galactose). Net ATP from glycolysis ATP from oxidative decarboxylation (if applicable) ATP formed from Krebs cycle (if applicable) Total net ATPConcerning the citric acid cycle (TCA cycle), which of the following statements is false? a. Substrate level phosphorylation in the TCA cycle produces 2 GTP per the oxidation of a single glucose molecule b. The TCA cycle generates electron carriers c. Oxidation of pyruvate in the matrix of the mitochondria produces sixteen total electrons d. The fate of the carbonyl carbon of acetyl CoAin the TCA cycle is to be lost as CO2 in the second turn e. Radiolabeling of the methyl carbon of pyruvate in the TCA cycle shows the carbon signal to split through successive turnsAssuming that all the carbon skeleton of alanine is completely oxidized and released asCO2. How many ATPs will be produced (including all ATPs produced by both substrate-levelphosphorylation and oxidative phosphorylation; please ignore the metabolism of nitrogen that isreleased from alanine)?a. 10b. 11.5c. 12.5d. 22.5e. Depending on which shuttle is used to transport NADH into mitochondria from cytosol