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Based on this alignment, you can identify areas of the protein sequences that are key regions of the proteins. If you were to design a targeted mutagenesis programme to try to disrupt the activity of the proteins in this current alignment as part of a research programme, which THREE amino acid sequence regions (motifs) would you target? List your sequence motifs in order of preference (number 1 being your most confident target). Explain your reasoning for each motif

Based on this alignment, you can identify areas of the protein sequences that are key regions of the proteins. If you were to design a targeted mutagenesis programme to try to disrupt the activity of the proteins in this current alignment as part of a research programme, which THREE amino acid sequence regions (motifs) would you target? List your sequence motifs in order of preference (number 1 being your most confident target). Explain your reasoning for each motif

Biology Today and Tomorrow without Physiology (MindTap Course List)
Biology Today and Tomorrow without Physiology (MindTap Course List)
5th Edition
ISBN:9781305117396
Author:Cecie Starr, Christine Evers, Lisa Starr
Publisher:Cecie Starr, Christine Evers, Lisa Starr
Chapter7: Gene Expression And Control
Section: Chapter Questions
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Based on this alignment, you can identify areas of the protein sequences that are key regions of the proteins. If you were to design a targeted mutagenesis programme to try to disrupt the activity of the proteins in this current alignment as part of a research programme, which THREE amino acid sequence regions (motifs) would you target? List your sequence motifs in order of preference (number 1 being your most confident target). Explain your reasoning for each motif

CLUSTAL O (1.2.4) multiple sequence alignment SEQUENCE_15 SEQUENCE_20 SEQUENCE 13 SEQUENCE_12 SEQUENCE 14 SEQUENCE 16 SEQUENCE 18 SEQUENCE_19 SEQUENCE 11 SEQUENCE 17 -MSRSGKSLTKYKIVFLGEQGVGKTSLITRFMYDTFDDHYQATIGIDF 47 MIVKLLQEIQVAQNTEEYDMEATIKVVLLGDSSVGKTSIVTRLKSGKFLAKHAATIGAAF 60 --MLQFKLVLLGDSSVGKSSIVHRFVKDTFDELRESTIGAAF 40 -MNTSVTSIKLVLLGEAAVGKSSIVLRFVSNDFAENKEPTIGAAF 44 -MDKHTAAIPTLTIKVVLLGESAVGKSSIVLRFVSDDFKESKEPTIGAAF 49 -M--SSRKKNILKVIILGDSGVGKTSLMHRYVNDKYSQQYKATIGADF 45 -MSSEDY--GYDYDLLFKIVLIGDSGVGKSNLLSRFTKNEFNMDSKSTIGVEF 50 -MSNEDY--GYDYDYLFKIVLIGDSGVGKSNLLSRFTTDEFNIESKSTIGVEF 50 -M--NSEYDYLFKLLLIGNSGVGKSCLLLRFSDDTYTNDYISTIGVDF 45 -MSGLRTVSASSGN--GKSYDSIMKILLIGDSGVGKSCLLVRFVEDKFNPSFITTIGIDF 57 *::::*: ,***: :: * • : *** SEQUENCE_15 SEQUENCE 20 LSKTMYLD- 98 -DKTI-RLQLWDTAGQERFRSLIPSYIRDSRVAIIVYDITKRKSF ITKTIEVPSNDSS--TEKRI-HMEIWDTAGQERYKSLVPMYYRDANIALIVFELGDVSSL 117 SEQUENCE_13 SEQUENCE_12 SEQUENCE_14 SEQUENCE_16 SEQUENCE 18 SEQUENCE 19 SEQUENCE_11 SEQUENCE 17 LSQSITIHPNDGNETKDVVI-KFEIWDTAGQERYKSLAPMYYRNANAALVVYDITQEDSL 99 LTQRVTIN--- --EHTV-KFEIWDTAGQERFASLAPMYYRNAQAALVVYDVTKPQSF 95 LTKRITRD- -GKVI-KFEIWDTAGQERFAPLAPMYYRNAQAALVVFDVTNEGSF 100 -GDKVATMQVWDTAGQERFQSLGVAFYRGADCCVLVYDVTNASSF -GKRI-KAQIWDTAGQERYRAITSAYYRGAVGALIVYDISKSSSY -NKKI-KAQIWDTAGQERYRAITSAYYRGAVGALIVYDISKSSSY LTKEVTVD- 97 ATRTLEID- 101 ATRTIEVE- 101 KIKTVELD- -GKTV-KLQIWDTAGQERFRTITSSYYRGSHGIIIVYDVTDQESF 96 ΚΙKTVDIN- GKKV-KLQLWDTAGQERFRTITTAYYRGAMGIILVYDVTDERTF 108 : : ::********: : *.: ::*::: SEQUENCE 15 SEQUENCE 20 SEQUENCE 13 SEQUENCE 12 SEQUENCE 14 SEQUENCE 16 SEQUENCE 18 SEQUENCE_19 SEQUENCE_11 SEQUENCE 17 EYIDKWIEDVKNERGD-- -ENVILCIVGNKSDLSDERQ- -IS 135 152 QCAKTWFQDLQDRAQG---TQ--VIIVGNKYDLVCEEHSG- QKARNWVDELKNKVGD-- 156 -DDLVIYLLGNKVDLCQETPSTETSPDSNEGGDEEQKVRAIS --DIIIALVGNKIDMLQEGG- IKARHWVKELHEQASK- ERK---VA 134 YKAQNWVEELHEKVGH----DIVIALVGNKMDLLNNDD- EΕΝENRΑMΚ 142 ENIKSWRDEFLVHANVNSPETFPFVILGNKIDAEESKK- -IVS 138 ENCNHWLSELRENADD----NVAVGLIGNKSDLAHLRA- -V-P 137 ENCNHWLTELRENADD-- -NVAVGLIGNKSDLAHLRA- -V-P 137 NGVKMWLQEIDRYATS- -TVLKLLVGNKCDLKDKR- -V-E 132 TNIKQWFKTVNEHAND----EAQLLLVGNKSDMET-RV- V-T 143 :: 大 SEQUENCE_15 SEQUENCE_20 SEQUENCE 13 SEQUENCE 12 SEQUENCE 14 TEEGEKKAKLLGAKIFMETSTKAGYNVKALFKKIAKSLPEFQNSESTPLDSEN----ANS 191 --EVTIPAEL-QGLPYVAVSAKTGYNFDTLNKIIISLVPESQFK-TLSKNNE- 200 TEEAKQYAQE-QGLLFREVSAKTGEGVKEIFQDIGEKLYDLKKDEILSKONRQ- -IGG 211 187 REEGEKLAEE-KGLLFFETSAKTGENVNDVFLGIGEKIPLKTAEEQNSASNE- APAVQNLCER-ENLLYFEASAKTGENIYQIFQTLGEKVPCPEQNTRQSSTHD- --RE -RTI 196 SEQUENCE 16 SEQUENCE 18 SEQUENCE_19 SEQUENCE_1i SEQUENCE 17 EKSAQELAKSLGDIPLFLTSAKNAINVDTAFEEIARSALQQNQADTEAFEDD- 190 TEESKTFAQE-NQLLFTETSALNSENVDKAFEELINTIYQKVSKHQMDLGDSSANGNANG 196 195 TDEAKNFAME-NQMLFTETSALNSDNVDKAFRELIVAIFQMVSKHQVDLSGSGTN-NMGS YDVAKEFADA-NKMPFLETSALDSTNVEDAFLTMARQIKESMSQQNLNETTQKK- 185 ADQGEALAKE-LGIPFIESSAKNDDNVNEIFFTLAKLIQEKIDSNKLVGVGNG- 195 *: SEQUENCE 15 SEQUENCE 20 215 A-NQNKPGVIDIS-TAEEQEQSACQC- --QG--NILE-INKKKSGSGCIC- 218 SEQUENCE 13 G-NNGQVDINLQR-PSTNDPTSCCS-- 234 SEQUENCE 12 SEQUENCE 14 SEQUENCE_16 SEQUENCE_18 SEQUENCE_19 SEQUENCE_11 SEQUENCE 17 S-NNQRVDLNAAN--DGTSANSACSC- 210 T-DNQRIDLESTT-VESTRETGGCNC- 220 --YNDAINIRLDG-- ENNSCSC- 208 ASAPNGPTISLTPTPNENKKANGNNCC 223 NGAPKGPTISLTPAPKEDKKKKSSNCC 222 ---EDKGNVNLKG---QSLTNTGGGCC 206 ----KEGNISINS---GSGNSSKSNCC 215
Transcribed Image Text:CLUSTAL O (1.2.4) multiple sequence alignment SEQUENCE_15 SEQUENCE_20 SEQUENCE 13 SEQUENCE_12 SEQUENCE 14 SEQUENCE 16 SEQUENCE 18 SEQUENCE_19 SEQUENCE 11 SEQUENCE 17 -MSRSGKSLTKYKIVFLGEQGVGKTSLITRFMYDTFDDHYQATIGIDF 47 MIVKLLQEIQVAQNTEEYDMEATIKVVLLGDSSVGKTSIVTRLKSGKFLAKHAATIGAAF 60 --MLQFKLVLLGDSSVGKSSIVHRFVKDTFDELRESTIGAAF 40 -MNTSVTSIKLVLLGEAAVGKSSIVLRFVSNDFAENKEPTIGAAF 44 -MDKHTAAIPTLTIKVVLLGESAVGKSSIVLRFVSDDFKESKEPTIGAAF 49 -M--SSRKKNILKVIILGDSGVGKTSLMHRYVNDKYSQQYKATIGADF 45 -MSSEDY--GYDYDLLFKIVLIGDSGVGKSNLLSRFTKNEFNMDSKSTIGVEF 50 -MSNEDY--GYDYDYLFKIVLIGDSGVGKSNLLSRFTTDEFNIESKSTIGVEF 50 -M--NSEYDYLFKLLLIGNSGVGKSCLLLRFSDDTYTNDYISTIGVDF 45 -MSGLRTVSASSGN--GKSYDSIMKILLIGDSGVGKSCLLVRFVEDKFNPSFITTIGIDF 57 *::::*: ,***: :: * • : *** SEQUENCE_15 SEQUENCE 20 LSKTMYLD- 98 -DKTI-RLQLWDTAGQERFRSLIPSYIRDSRVAIIVYDITKRKSF ITKTIEVPSNDSS--TEKRI-HMEIWDTAGQERYKSLVPMYYRDANIALIVFELGDVSSL 117 SEQUENCE_13 SEQUENCE_12 SEQUENCE_14 SEQUENCE_16 SEQUENCE 18 SEQUENCE 19 SEQUENCE_11 SEQUENCE 17 LSQSITIHPNDGNETKDVVI-KFEIWDTAGQERYKSLAPMYYRNANAALVVYDITQEDSL 99 LTQRVTIN--- --EHTV-KFEIWDTAGQERFASLAPMYYRNAQAALVVYDVTKPQSF 95 LTKRITRD- -GKVI-KFEIWDTAGQERFAPLAPMYYRNAQAALVVFDVTNEGSF 100 -GDKVATMQVWDTAGQERFQSLGVAFYRGADCCVLVYDVTNASSF -GKRI-KAQIWDTAGQERYRAITSAYYRGAVGALIVYDISKSSSY -NKKI-KAQIWDTAGQERYRAITSAYYRGAVGALIVYDISKSSSY LTKEVTVD- 97 ATRTLEID- 101 ATRTIEVE- 101 KIKTVELD- -GKTV-KLQIWDTAGQERFRTITSSYYRGSHGIIIVYDVTDQESF 96 ΚΙKTVDIN- GKKV-KLQLWDTAGQERFRTITTAYYRGAMGIILVYDVTDERTF 108 : : ::********: : *.: ::*::: SEQUENCE 15 SEQUENCE 20 SEQUENCE 13 SEQUENCE 12 SEQUENCE 14 SEQUENCE 16 SEQUENCE 18 SEQUENCE_19 SEQUENCE_11 SEQUENCE 17 EYIDKWIEDVKNERGD-- -ENVILCIVGNKSDLSDERQ- -IS 135 152 QCAKTWFQDLQDRAQG---TQ--VIIVGNKYDLVCEEHSG- QKARNWVDELKNKVGD-- 156 -DDLVIYLLGNKVDLCQETPSTETSPDSNEGGDEEQKVRAIS --DIIIALVGNKIDMLQEGG- IKARHWVKELHEQASK- ERK---VA 134 YKAQNWVEELHEKVGH----DIVIALVGNKMDLLNNDD- EΕΝENRΑMΚ 142 ENIKSWRDEFLVHANVNSPETFPFVILGNKIDAEESKK- -IVS 138 ENCNHWLSELRENADD----NVAVGLIGNKSDLAHLRA- -V-P 137 ENCNHWLTELRENADD-- -NVAVGLIGNKSDLAHLRA- -V-P 137 NGVKMWLQEIDRYATS- -TVLKLLVGNKCDLKDKR- -V-E 132 TNIKQWFKTVNEHAND----EAQLLLVGNKSDMET-RV- V-T 143 :: 大 SEQUENCE_15 SEQUENCE_20 SEQUENCE 13 SEQUENCE 12 SEQUENCE 14 TEEGEKKAKLLGAKIFMETSTKAGYNVKALFKKIAKSLPEFQNSESTPLDSEN----ANS 191 --EVTIPAEL-QGLPYVAVSAKTGYNFDTLNKIIISLVPESQFK-TLSKNNE- 200 TEEAKQYAQE-QGLLFREVSAKTGEGVKEIFQDIGEKLYDLKKDEILSKONRQ- -IGG 211 187 REEGEKLAEE-KGLLFFETSAKTGENVNDVFLGIGEKIPLKTAEEQNSASNE- APAVQNLCER-ENLLYFEASAKTGENIYQIFQTLGEKVPCPEQNTRQSSTHD- --RE -RTI 196 SEQUENCE 16 SEQUENCE 18 SEQUENCE_19 SEQUENCE_1i SEQUENCE 17 EKSAQELAKSLGDIPLFLTSAKNAINVDTAFEEIARSALQQNQADTEAFEDD- 190 TEESKTFAQE-NQLLFTETSALNSENVDKAFEELINTIYQKVSKHQMDLGDSSANGNANG 196 195 TDEAKNFAME-NQMLFTETSALNSDNVDKAFRELIVAIFQMVSKHQVDLSGSGTN-NMGS YDVAKEFADA-NKMPFLETSALDSTNVEDAFLTMARQIKESMSQQNLNETTQKK- 185 ADQGEALAKE-LGIPFIESSAKNDDNVNEIFFTLAKLIQEKIDSNKLVGVGNG- 195 *: SEQUENCE 15 SEQUENCE 20 215 A-NQNKPGVIDIS-TAEEQEQSACQC- --QG--NILE-INKKKSGSGCIC- 218 SEQUENCE 13 G-NNGQVDINLQR-PSTNDPTSCCS-- 234 SEQUENCE 12 SEQUENCE 14 SEQUENCE_16 SEQUENCE_18 SEQUENCE_19 SEQUENCE_11 SEQUENCE 17 S-NNQRVDLNAAN--DGTSANSACSC- 210 T-DNQRIDLESTT-VESTRETGGCNC- 220 --YNDAINIRLDG-- ENNSCSC- 208 ASAPNGPTISLTPTPNENKKANGNNCC 223 NGAPKGPTISLTPAPKEDKKKKSSNCC 222 ---EDKGNVNLKG---QSLTNTGGGCC 206 ----KEGNISINS---GSGNSSKSNCC 215
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