BHI TTGTAAAACGACGGCCAGTGAGCGCGCGTAATACGACT M13-20 primer binding site Bsp 1061 goi Primer design worksheet T7 Promoter ...KS primer binding site Primer 17 primer binding site Hind EcoRV EcoRI Pal Eco01091 Xhol Kpn Dra CACTATAGGGCGAATTGGGTACCGGGCCCCCCCTCGAGGTCGAC... Acc Xbal Smal BamHI Spel ...GGTATCGATAAGCTTGATATCGAATTCCTGCAGCCCGGGGGATCCACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTC... SK primer binding site KS primer binding site... Socl Not Eagl Batx I Sac II The diagram above shows a portion of the pSK+ multicloning site. For this worksheet, you need to design primers that could be used to amplify a gene cloned into the EcoRI cloning site. There are 4 primer sites indicated on the diagram. The primers need to be designed to bind to each of the primer binding sites indicated on the diagram. Fill in the following information: Identify all possible Forward primers and write out their sequence: 5'-3' sequence

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Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.