pZERO®-1 is a 2808 bp cloning vector from Invitrogen. This vector allows effective selectionof positive recombinants via disruption of the lethal gene, ccdB. Besides the lethal gene, it hasan additional Zeocin resistance gene for tighter screening control. The Zeocin resistance genein this vector is certainly more advantageous over ampicillin resistance gene. Give its THREE(3) advantages.

The PET sequences are simentensously traced to the genome assembly to define the boundaries of…

Answered: pZERO®-1 is a 2808 bp cloning vector…

pZERO®-1 is a 2808 bp cloning vector from Invitrogen. This vector allows effective selection of positive recombinants via disruption of the lethal gene, ccdB. Besides the lethal gene, it has

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1ITD

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Concept explainers

Bacterial Genomics

The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.

Transformation Experiment in Bacteria

In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.

Plasmids and Vectors

The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.

Question

pZERO®-1 is a 2808 bp cloning vector from Invitrogen. This vector allows effective selection
of positive recombinants via disruption of the lethal gene, ccdB. Besides the lethal gene, it has
an additional Zeocin resistance gene for tighter screening control. The Zeocin resistance gene
in this vector is certainly more advantageous over ampicillin resistance gene. Give its THREE
(3) advantages.

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