7. You want to separate these four proteins in a mixture: Isoelectric point Mol. Weight (kDa) Protein A 4.1 80 Protein B 9.0 81 Protein C 8.8 37 Protein D 3.9 172 Which of the following would be the best order in which to run chromatography columns? A) Anion-exchange at pH 3, then size exclusion B) Cation-exchange at pH 3, then size exclusion C) Cation-exchange at pH 3, then anion-exchange at pH 3 D) Anion-exchange at pH 3, then cation-exchange at pH 3 E) Size exclusion, then cation-exchange at pH 3 F) Size exclusion, then anion-exchange at pH 3
7. You want to separate these four proteins in a mixture: Isoelectric point Mol. Weight (kDa) Protein A 4.1 80 Protein B 9.0 81 Protein C 8.8 37 Protein D 3.9 172 Which of the following would be the best order in which to run chromatography columns? A) Anion-exchange at pH 3, then size exclusion B) Cation-exchange at pH 3, then size exclusion C) Cation-exchange at pH 3, then anion-exchange at pH 3 D) Anion-exchange at pH 3, then cation-exchange at pH 3 E) Size exclusion, then cation-exchange at pH 3 F) Size exclusion, then anion-exchange at pH 3
Anatomy & Physiology
1st Edition
ISBN:9781938168130
Author:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Publisher:Kelly A. Young, James A. Wise, Peter DeSaix, Dean H. Kruse, Brandon Poe, Eddie Johnson, Jody E. Johnson, Oksana Korol, J. Gordon Betts, Mark Womble
Chapter2: The Chemical Level Of Organization
Section: Chapter Questions
Problem 12RQ: A substance formed of crystals of equal numbers of cations and anions held together by ionic bonds...
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Transcribed Image Text:7. You want to separate these four proteins in a mixture:
Isoelectric point
Mol. Weight (kDa)
Protein A
4.1
80
Protein B
9.0
81
Protein C
8.8
37
Protein D
3.9
172
Which of the following would be the best order in which to run chromatography columns?
A) Anion-exchange at pH 3, then size exclusion
B) Cation-exchange at pH 3, then size exclusion
C) Cation-exchange at pH 3, then anion-exchange at pH 3
D) Anion-exchange at pH 3, then cation-exchange at pH 3
E) Size exclusion, then cation-exchange at pH 3
F) Size exclusion, then anion-exchange at pH 3
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