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- 1. Know the structures of DNP-glycine and of the chromophore associated with cytochrome C. Low Salt Volume of blue band = 5.2 ml (Void Volume) Volume of yellow band = 2.6ml Volume of red band = 11ml High Salt Volume of blue band = 4.2 ml -> Void Volume Volume of yellow band = 5.2 Volume of red band = 1.2 2. What is the exclusion limit and the fractionation range for CM-Sephadex resin used? 3. What are the ion exchange reactions which occur when the coloured mixture is first applied to the column under low salt conditions? What exchange reactions occur upon the change of buffer from low salt to high salt concentrations? 4. What were the values for Vo and Vi for this column? Where the values for Vo and Vi the same under both conditions? 5. Explain the order in which the various components of the mixture elute under each condition in terms of their physical properties and the nature of the CMSephadex G-50 column. Would the same order be observed with a G-50 Sephadex column (i.e. no…1. provide three reasons why most of the research on carotenoids concentrates on b-carotene. 2. draw the retinol ester that has stearic acid as the carboxylic acid portion of its structure.5 25) Although the UV280 extinction coefficient of BSA is well known, why was the BCA assay employed to measure protein concentration of the folate labeled BSA? Please provide an explanation. (C) Would the use of reducing agents have an affect on the tertiary structure of BSA—please explain your answer (you will need to investigate the structure of BSA beyond this article)?
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- 1)Since bromelain is an endopeptidase, what types of bonds does it work on? 2)Since bromelain is an endopeptidase, what types of bonds does it work on?describe a detailed experimental procedure for the chemical synthesis of proteins with the α-ketoacid hydroxylamine (KAHA ligation), using (S)-5-oxaproline (Opr) as a key building block.1. Glycosphingolipids function via cis regulation and trans recognition. Explain these terms and provide examples of each.