Which of the following directly indicates that a bacterial cell has taken up your cloning vector? O Growth on an antibiotic O Ability to cut with many different restriction endonucleases. O Blue colonies on X-gal O White colonies on X-gal

Which of the following directly indicates that a bacterial cell has taken up your cloning vector? O Growth on an antibiotic O Ability to cut with many different restriction endonucleases. O Blue colonies on X-gal O White colonies on X-gal

Name: 14 Which of the following directly indicates that a bacterial cell has
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Description: 14 Which of the following directly indicates that a bacterial cell has taken up your cloning vector?O Growth on an antibioticO Ability to cut with many different restriction endonucleases.O Blue colonies on X-galO White colonies on X-gal

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 6QP

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The recognition site of some restriction enzymes are shown. Which will produce sticky ends? A. EcoRI G↓AATTC. B. SmaI CCC↓GGG. C. XhoI C↓TCGAG. D. Both EcoRI and XhoI. E. All the enzymes shown will leave sticky ends.
What carries a gene from one organism into a bacteria cell?a. a plasmidb. an electrophoresis gelc. a restriction enzymed. polymerase chain reaction
The genomic DNA of a bacterial cell is not destroyed by the cell’s own restriction enzymes because choose 1 answer below: the bacterial DNA is too small to contain the recognition sequence for the enzymes. the restriction sites are occupied by histones. the genome is protected by the nuclear membrane. the pH in the bacterial cell does not allow the restriction enzymes to function. None of the above
Cloning vector contains a multiple cloning site (MCS) or polylinker containing multiple unique restriction sites. It also contains markers that allow for rapid selection of colonies with insert-containing plasmids in them. The 1st statement is True and the 2nd statement is False. The 1st statement is False and the 2nd statement is True. Both Staments are True. Both statements are False.
The genomic DNA of a bacterial cell is NOT destroyed by the cell’s own restriction enzymes because Choose an answer from below: the bacterial DNA is too small to contain the recognition sequence for the enzymes. the restriction sites are occupied by histones. the genome is protected by the nuclear membrane. the restriction recognition sequences in the genome are protected by a microRNA. none of the above
Which of the following is or are required for cloning a gene?a. restriction endonucleaseb. vector c. cloning host d. a and b e. all of these
The restriction enzymes used in gene-cloning experiments, which generates sticky ends that can .a. cut the DNA, enter bacterial cellsb. cut the DNA, hydrogen bond with complementary sticky endsc. methylate DNA, enter bacterial cellsd. methylate DNA, hydrogen bond with complementarysticky ends
Which of the following term is associated with DNA finger printing?a) Hybridomab) Site specific mutagenesisc) Shotgun cloningd) RFLP
Which of the enzymes from the following list wouldyou need to make a recombinant DNA molecule? Whatis the function of those enzyme(s) in the process?a. DNA polymeraseb. RNA polymerasec. A restriction enzymed. DNA ligasee. An aminoacyl-tRNA synthetasef. Peptidyl transferaseg. Reverse transcriptase
Which of the enzymes from the following list wouldyou need to sequence DNA? What is the function ofthose enzyme(s) in the process?a. DNA polymeraseb. RNA polymerasec. A restriction enzymed. DNA ligasee. An aminoacyl-tRNA synthetasef. Peptidyl transferaseg. Reverse transcriptase
Features of restriction enzymes include the following, except __________ . a. They are produced by both prokaryotes and eukaryotesb. may be type IIc. make single and double stranded breaks in DNAd. operate at a single temperaturee. Are used in nature to destroy bacteriophage DNA
Which statement is NOT the function of restriction enzyme? Group of answer choices It cuts the vector’s plasmid and the desired gene from the original DNA. It ligates the desired to gene to vector’s plasmid. It acts as scissors of DNA that cut along a specific sequence. It serves as degrading proteins that cut plasmid on specific target site.