Which of the following is required to make complementary DNA (CDNA) from RNA? reverse transcriptase DNA ligase DNA ligase gene cloning restriction enzymes (endonucleases)

Q: Which of the following enzymes are needed to introduce foreignDNA into a vector?a. DNA gyrase and…

A: The tightly packed genetic material located in the nucleus of a living organism which is made up of…

Q: In the given below option which of the following are considered as the main tools for DNA…

A: DNA technology alters the phenotype of the organism through a genetically altered vector.  It…

Q: Identify the correct sequence of steps involved in cutting and reassembling DNA molecules to make…

A: Answer: Recombinant DNA is the DNA which is made in the laboratory  by combining two genetic…

Q: In recombinant DNA technology, restriction enzymes cleave the DNA at a specific site known as:

A: Restriction enzyme, also known as restriction endonuclease, is a bacterial protein that cleaves DNA…

Q: A ____________ is required to transfer DNA sequences from one organism to another. reporter gene…

A: A reporter gene is a gene that is used as a selectable marker in gene cloning to identify whether a…

Q: Which of the following enzyme is used in PCR?a) Taq DNA polymeraseb) HRPc) EcoRId) EcoRII

A: Polymerase chain reaction (PCR) is a laboratory technique, which is used to make multiple copies of…

Q: Enzymes of bacterial origin used in a wide variety of techniques are: ligases restriction…

A: DNA polymerase

Q: Which of the following enzymes are needed to introduce foreign DNA into a vector? a. DNA gyrase and…

A: DNA (deoxyribonucleic acid) is the genetic material in most organisms except few viruses.…

Q: Draw an Illustration of the steps in restriction digestion and PCR [ Please make it clean and…

A: Restriction digestion is the process of cutting the DNA sequence at a particular nucleotide sequence…

Q: Enzymes used to cut genes in recombinant DNA research are known as ____.   a. DNA polymerases   b.…

A: Enzymes  Protein molecules that act as catalyst in a biological processe or reaction.

Q: Which of the following types of mutations would be produced by repair of a CRISPR-targeted double…

A: Non Homologous End joining is described as the pathway required for the repairing of those double…

Q: A restriction enzyme digests DNA into fragments. Name the technique used to check the progression of…

A: DNA and RNA are nucleic acids that are found in living cells. Almost all living cells contain both…

Q: Most prokaryotic organisms produce one or more enzymes to defend themselves against the infection by…

A: Bacteria constitute a wide domain of prokaryotic microbes with majorly varying lengths and a number…

Q: Which of the following is not required in order for a plasmid to be used in recombinant DNA…

A: A plasmid is a single-stranded, circular DNA molecule that is different from the chromosomal DNA of…

Q: What is the fastest method to determine if a genetic disorder is due to a mutation at a palindromic…

A: Mutations This refers to the alterations in the sequence of DNA. These are caused from the mistakes…

Q: An viral enzyme that can be used to create complementary cDNA is DNA polymerase. reverse…

A: The genome of an organism is defined as the whole heredity information encoded in the genetic…

Q: Which of the following is NOT an advantage of the PCR method over traditional cloning methods for…

A: PCR or polymerase chain reaction refers to the method by which a particular DNA (deoxyribonucleic…

Q: Which of the following ingredients does not belong in a PCR reaction? DNA polymerase B) Primer…

A: Polymerase chain reaction is a method used to produce millions of copies of any DNA sample and hence…

Q: Select the statement below that is FALSE regarding DNA and DNA Fingerprinting: Select an answer and…

A: Each individual is unique because of his DNA sequences.  Determining the DNA sequences by cutting…

Q: You are cloning a gene from a eukaryotic organism into a plasmid to produce a recombinant protein in…

A: The biological process by which naked DNA (deoxyribonucleic acid) gets transferred from one…

Q: Features of restriction enzymes include the following, except  __________ . a. They are produced…

A: Restriction enzyme is a group of enzymes which involves the cleaving of DNA molecule at a specific…

Q: The enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy.…

A: DNA cloning is a process in which clones / copies of DNA are formed . DNA sequencing is a technique…

Q: Modern researchers can manipulate genetic material to alter genes and blend plant, animal and…

A: Genetic engineering: Also called genetic modification, it is defined as the process in which the DNA…

Q: What are restriction enzymes and what do they do? Complete the chart Driginal DNA sequence 5' TGA…

A: Restriction enzymes bind and cleave double-stranded DNA. There are three categories of restriction…

Q: Once separated from other cellular components, extracted DNA can be used for a variety of purposes.…

A: Introduction:- Biotechnology relies heavily on the extraction of DNA. It serves as the foundation…

Q: Select all the following statements that are correct regarding agarose gel electrophoresis and…

A: Agarose gel electrophoresis is a process used for separation of DNA fragments. Steps of agarose gel…

Q: True or False: Restriction enzymes normally prevent viral DNA or RNA entry in bacteria.

A: A bacterium utilizes a restriction protein to defend against bacterial infections called…

Q: Pcr primers are:  single strand 15-25 bases long incorporated into the newly synthesized DNA…

A: PCR stands for polymerase chain reaction. It is one of the technique of biotechnology.

Q: Which of the following can be termed as a restriction modification system?a) Restriction…

A: Enzymes are the biological catalysts which are proteinaceous in nature. The function of an enzyme is…

Q: What is the natural purpose of restriction endonucleases in bacteria? O To digest the bacterial…

A: According to the question, we have to find out what is the natural purpose of restriction…

Q: Which of the following is most likely to be a restriction enzyme recognition sequence? O AAAAAA O…

A: A palindromic sequence is a sequence of nucleic acids within double helix of DNA and / or RNA that…

Q: Why does one need a vector to clone a DNA

A: Cloning is the method of generating the number of copies of desired DNA I  the host.

Q: Genes of Interest can be selected from a genomic library by using (a) cloning vectors o) DNA probes…

A: Vector refers to a DNA molecule which is used as a vehicle to carry genetic material from one cell…

Q: After DNA unwinds and becomes single-stranded in a PCR reaction, the temperature is lowered to allow…

A: Polymerase chain reaction ( PCR) is a technique that are used in amplification of specific DNA…

Q: The Southern blot procedure was developed so that: DNA fragments that have been subjected to…

A: Southern blotting is the technique for the detection of specific DNA fragments. The detailed…

Q: Which of the enzymes from the following list wouldyou need to sequence DNA? What is the function…

A: DNA sequencing is a process in which the sequence of the nitrogenous bases is determined in the…

Q: Which of the following is a desirable characteristic for a cloning plasmid? a site at which…

A: Plasmids are self replicating extra chromosomal DNA. They are circular in shape and are found in…

Q: MOLECULAR CLONING • Molecular cloning is the laboratory process used to create recombinant DNA. • It…

A: Recombinant DNA: it is a method of combining two distinct DNA (molecule) sequences, which may or may…

Q: The complete genetic information of a person is isolated from some of her cells, cut with…

A: Genetics is a part of science worried about the investigation of genes, genetic variety, and…

Q: Which of the enzymes from the following list wouldyou need to make a recombinant DNA molecule?…

A: The recombinant DNA is where the two or more DNA molecules are integrated into a vector plasmid to…

Q: Which one of the following statements about restriction endonucleases is true? all restriction…

A: A restriction enzyme or restriction endonuclease is an enzyme that cleaves/cuts DNA into fragments…

Q: The enzymes mentioned below are used as tools during cloning, DNA sequencing and/or gene therapy.…

A: Enzymes are macromolecular biological catalysts that accelerate chemical reactions. The molecules…

Q: What molecular biology technique is used to amplify DNA from an evidence sample?     Reverse dot…

A: Gene Amplification:  Gene amplification is a term that describes a variety of natural and artificial…

Q: If a restriction endonuclease recognizes and cleaves a linear piece of DNA at 5 distinct places, how…

A: DNA and RNA are nucleic acids present in the organisms. DNA is the deoxy ribose nucleic acid whereas…

Q: Which of the following tools of DNA technology is incorrectlypaired with its use?(A)…

A: As in the recent times, the approach towards genetics has gone up a high level. Various techniques…

Q: Which of the following does NOT require a primer? A) PCR-based viral test B) cloning a human gene…

A: It is the process of producing individuals with identical or virtually identical DNA, either…

Q: Two techniques used to make large amounts of a segment of DNA or gene of interest are: polymerase…

A: The two techniques used to make large amounts of a segment of DNA or gene of interest are:…

Q: n the given below option which of the following are considered as the main tools for DNA technology?…

A: The creation of desired DNA molecules using DNA technology is a method for improving the expression…

Q: Which of the following cuts DNA like molecular scissors? Clustered regularly interpaced short…

A: Clustered regularly interspaced short palindromic repeats that are known as CRISPR are family of DNA…

Q: The genomic DNA of a bacterial cell is NOT destroyed by the cell’s own restriction enzymes because

A: Restriction enzymes Restriction enzyme or restrictions endonuclease are use to cleave DNA or RNA at…

Genome annotation

The technique of linking biological information to genome sequences is termed genome annotation. Gene annotation is the method of identifying gene locations and coding sections. It helps us understand what these genes are doing in the body through establishing structural characteristics and linking them to the actions of various proteins.