Which of the following statements best describes the major role of plasmid in DNA cloning? Carry the foreign DNA into the host cell O Make the foreign DNA susceptible to digestion with enzymes. O Identify the host cell that has taken up the gene of interest. O Identify the source of DNA as foreign.

Which of the following statements best describes the major role of plasmid in DNA cloning? Carry the foreign DNA into the host cell O Make the foreign DNA susceptible to digestion with enzymes. O Identify the host cell that has taken up the gene of interest. O Identify the source of DNA as foreign.

Name: 10 Which of the following statements best describes the major role of
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Description: 10 Which of the following statements best describes the major role of plasmid in DNA cloning?Carry the foreign DNA into the host cellO Make the foreign DNA susceptible to digestion with enzymes.O Identify the host cell that has taken up the gene of i

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 10QP: Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands...

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What carries a gene from one organism into a bacteria cell? a. a plasmid b. an electrophoresis gel c. a restriction enzyme d. polymerase chain reaction
Cloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymerase
Which of the following describes an advantage of using a recombinant plasmid for DNA cloning over PCR? A. PCR is more likely to have errors introduced in the copying process. B. Recombinant DNA plasmids are able to create large amounts of copies more quickly than PCR. C. PCR can only be conducted in eukaryotic cells. D. PCR requires prior knowledge of the sequence in question, while a recombinant plasmid does not.
Which of the following would be a good application of plasmid transformation? 1) to make copies of DNA 2) to isolate a change in a single nucleotide 3) to separate DNA fragments 4) to sequence DNA
Which of the following would you need to clone a human gene? Choose all that apply a) A surrogate mother b) A plasmid vector (or other type of DNA vector, e.g. phage, BAC) c) A donor goat nucleus d) A restriction enzyme e) Human DNA f) An enucleated egg g) DNA ligase
What occurs during the HIGH temperature step of PCR? A) DNA is cut by restriction endonucleases B) primers get degraded C) primers anneal to single-stranded DNA D) DNA is synthesized E) double stranded DNA separates into single stranded DNA
With the use of well-illustrated diagrams, reconstruct the entire cloning process by explaining different stages of the cloning process that involves the following:a. Isolation of target DNA fragments (often referred to as inserts)b. Ligation of inserts into the plasmid, creating recombinant molecules c. Transformation of recombinant plasmids into bacteria or other suitable host for propagationd. Screening/selection of hosts containing the intended recombinant plasmid. For this stage(d), discuss the importance of a second marker that can be used for screening of genomic DNA for colonies containing the pka-1 under the principle of insertional inactivation. This should be properly explained using all the attributes of the plasmid described above.
Which of the following would you need to clone a goat? Choose all that apply a) A restriction enzyme b) A surrogate mother c) A plasmid vector (or other type of DNA vector, e.g. phage, BAC) d) A donor goat nucleus e) Human DNA f) DNA ligase g) An enucleated egg
A genomic DNA library is made by using _____ to cut the target genome into small fragments, which are then amplified by PCR and ligated into plasmids.
Which of the following would you need to clone a human gene? Choose all that apply A restriction enzyme Human DNA A donor goat nucleus An enucleated egg A surrogate mother DNA ligase A plasmid vector (or other type of DNA vector, e.g. phage, BAC)
In order to insert foreign DNA into a plasmid for molecular cloning, the main components required include __________. (Check all that apply.) a selectable marker, usually antibiotic resistance a bacterial artificial chromosome one or more unique restriction sites an origin of replication
which of the following do researchers not need to use during vector cloning? a. a plasmid containing selectable marker genes such as beta galactosidase or ampicillin resistance genes. b. restriction enzymes. c. DNA polymerase d. a growth medium with carefully selected ingredients that take advantage of selectable markers. e. none of the above.