You use BamHI to cut open an empty plasmid vector and insert a gene. The following charts show the restriction digests of the empty plasmid and the plasmid containing the inserted gene. Use them to answer the questions below: Empty Plasmid BamHI 9.0Kb EcoRV 6.0Kb 3.0Kb BamHI + EcoRV 6.0Kb 2.0Kb |1.0Kb Plasmid with Insert BamHI 9.0Kb |1.ОКЬ EcoRV 6.0Kb 2.2Kb 1.8Kb What is the size of the empty plasmid? 9.0Kb What is the size of the insert? 1.0 KЬ From smallest to largest, what sizes of fragments would be created by digestion of the plasmid with insert by BamHI and Hindll1?

You use BamHI to cut open an empty plasmid vector and insert a gene. The following charts show the restriction digests of the empty plasmid and the plasmid containing the inserted gene. Use them to answer the questions below: Empty Plasmid BamHI 9.0Kb EcoRV 6.0Kb 3.0Kb BamHI + EcoRV 6.0Kb 2.0Kb |1.0Kb Plasmid with Insert BamHI 9.0Kb |1.ОКЬ EcoRV 6.0Kb 2.2Kb 1.8Kb What is the size of the empty plasmid? 9.0Kb What is the size of the insert? 1.0 KЬ From smallest to largest, what sizes of fragments would be created by digestion of the plasmid with insert by BamHI and Hindll1?

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 11QP: Cloning Genes Is a Multistep Process In cloning human DNA, why is it necessary to insert the DNA...

See similar textbooks

Similar questions

In relation to the use of restriction enzymes in recombinant DNA technology, answer the following: You have accidentally torn the labels off two tubes (tube A and tube B), each containing a different plasmid, now you do not know which plasmid is in which tube. Fortunately, you have restriction maps for both plasmids, shown in Figure below. You have the opportunity to test just one sample from one of your tubes. By utilizing agarose gel electrophoresis technique, which restriction enzyme OR combination of restriction enzymes would you use in this experiment to determine which plasmid is found in which tube?. (Hint: if you use Hind III restriction enzyme you are going to get ONE single fragment with a molecular size of → 0.5+0.3+0.2+0.4+1+1 = 3.4 kb).
Construct a restriction map for Plasmid X to show the recognition sites for two commonly used restriction enzymes: EcoRI and BamHI. Plasmid X undigested Plasmid X digested with EcoRI Plasmid X digested with BamHI Plasmid X digested with EcoRI and BamHI 2200 bp 1000 bp 1200 bp 400 bp 1800 bp 200 bp 400 bp 600 bp 1000 bp
When a 5-kb circular plasmid is digested with a restriction enzyme that has three recognition sites on the plasmid, how many bands can be visualized on an agarose gel? a)1 b)2 c)5 d)3
Assume that a plasmid is 4700 base pairs in length and has restriction sites for a given restriction enzyme at the following locations: 800, 1400, 2900, and 3600. List the fragments by size that are ! expected when the plasmid is fully digested the restriction enzyme.
This plasmid was digested using different restriction enzymes whose sites have been mapped. The plasmid is 7896 base pairs long. This is a long question so u can count this as two or even three but please answer the question? Determine the size (base pairs) and number of fragments that would be produced if the plasmid was digested with the following enzymes: a) EcoRI b) BamHI c) HindIII d) EcoRI and HindIII e)EcoRI, HindIII, and BamHI *Hint- this is actually an EASY question, since the restriction map is already drawn for you!
true or false if a restriction enzyme recognizes the restriction site, 5' AACGTT3', and the enzyme cuts between the second A and the C, this will produce a "sticky end," which is useful for cloning into a plasmid vector.
Define the following terms: Plasmid Restriction Enzyme Standard Curve
A DNA sequence is shown below, which includes a gene as marked. You have the restriction enzymes SalI and HindIII available to you to excise the gene prior to its incorporation into a plasmid vector. Which would you use to excise the gene?
________________ "cut" DNA at specific sequences and the resulting segment with sticky ends is "pasted" into a plasmid by ___________ to then be replicated in the host. Group of answer choices 1. Restriction enzymes; ethidium bromide 2 DNA polymerases; ligase 3 Restriction enzymes; ligase 4 Ligases; restriction enzymes
Using one of the options below, finish the following statement so that it is correct. Plasmids are _________ a. extrachromosomal, circular molecules of RNA b. extrachromosomal, linear molecules of DNA c. None of these options are correct d. extrachromosomal, circular molecules of DNA e. extrachromosomal, linear molecules of RNA
Assume that a circular plasmid is 3200 base pairs in length and has restriction sites for HindIII restriction enzyme at the following locations: 400, 700, 1400, 2600. Give the expected sizes of the restriction fragments following complete digestion.
Choose the one answer that fits best. The pGLO plasmid you used contains an araC gene, a GFP gene and a bla gene. If you performed the exact same experiment with a plasmid that contained a GFP gene and a bla gene, but was missing the araC gene, what would you expect to see on your four plates? a. The positive control would not have any bacterial lawn b. The negative control would have as many colonies as the experimental plate c. The LB/Amp/Ara plate would have some colonies but they would not be fluorescent under UV light d. The experimental plate would not have any colonies e. The LB/Amp plate containing E. coli (+)pGLO would be fluorescent