Briefly describe the main scientific principle(s) underlying the following techniques used in the Combating Disease practical (you may use diagrams if you wish): ammonium sulphate precipitation Dialysis Immunoelectrophoresis
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- Briefly describe the main scientific principle(s) underlying the following techniques used in the Combating Disease practical (you may use diagrams if you wish):
- ammonium sulphate precipitation
- Dialysis
- Immunoelectrophoresis
Step by step
Solved in 3 steps
- A surgical technologist working in the Central Sterile Processing Department is asked to run the steam sterilizer (autoclave) with a biological monitor for the first load of the day without any instrument trays or items. Which type of bacteria would be used to test the autoclave?Explain the following tests using Pseudomonas aeruginosa. Describe the purpose, reagents used and the results using Pseudomonas aeruginosa.the following are the steps, listed in random order, for utilizing koch's postulates. what should be the second step out of the four listed? a. a microorganism is found to be present in every case of a disease. b. the same microbe discovered previously is isolated from the purposely infected host c. the pathogen is isolated and grown in pure culture d. the same disease results after isolated organism is inoculated into healthy host
- Which ONE of the following is not taken into consideration when determining the ‘clinical breakpoint’? Select one: A. The drug MIC distribution for the wild-type bacterial population B. The microbiological outcome C. The drug Cmax value D. Pharmacodynamical parameters E. Elimination half-life of the drugPlease put the following types of organisms/pathogens into the correct order based on their resistance to general antimicrobial compounds. The MOST resistant description will be #1 and the LEAST resistant description will be #4. Gram positive bacteria Mycobacteria (contain mycolic acids/waxy lipids) Gram negative bacteria EndosporesBelow is a scenario requiring the use of laboratory equipment and instruments. A watery fecal sample is tested for the presence of Bacillus cereus and Vibrio cholerae. The sample is stored in a screw capped tube. You are tasked to grow the Bacillus in a solid medium while Vibrio in a liquid medium. Choices: A. Incubator, B Hanging Drop Slide , C.Petri Dish, D. Drying Oven, E. Bunsen Burner, F. Inoculating NeedlE , G. Volumetric Pipette, H. Screw-capped Tubes, I. Hot plate, J. Centrifuge, K. Inoculating Loop, L. Microscope, M. Beaker, N. Biosafety Cabinet 1. What could be used to determine if there are more bacteria in the sample aside from Bacillus and Vibrio? 2. What should you use if you want to observe the natural motility of Bacillus under a Brightfield microscope? 3. Where should you perform the task of sterilization and inoculation? 4.What should be used to transfer the sample to the broth? 5. What could be used to separate the solid from the liquid portion of the…
- Many antimicrobials were discovered in the first half of the 20th century. Match the early antimicrobial drugs with their description and/or target disease: salvarsan sulfanilamide penicillin prontosil Choices - first synthetic antimicrobial synthetic dye used to treat streptococcal and staphylococcal infections treatment for syphilis first discovered natural antibioticWhat therapeutic agent is used in the given conditionsChoose one specific way from each of the two methods of inhibiting the growth of microbes below. Describe the method, explain the mechanism of action of killing the microorganisms and give practical applications of your chosen ways. 1. Physical Methods - Heat, cold, dessication, radiation, ultrasonic waves, filtration, gaseous atmosphere 2. Chemical Methods- disinfectants, antiseptics
- If a patient suspected to have sepsis or meningitis, samples for bacterial testing should be taken before giving antibiotics. Explain why?Discuss the application of the following techniques in histopathology: 1.2.1. Immunohistochemistry 1.2.2. Fluorescent in situ hybridization 1.2.3. Nucleic acid amplificationlist 5 different antimicrobial agents, describe their mode of action and whether the are an HLD, ILD or LLD.
