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Briefly explain the difference between Wright Staining and H&E Staining (why use one over the other)
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- What major advantage does phase-contrast microscopy haveover staining?Describe how simple staining and fluorescence staining are similar and how they are different. What are the advantages of each?What are the pros and cons of using the TCE stain-free method versus a traditional gel staining method (CBB staining)?
- How are negative stains prepared?Name two applications for which this type of stain especially useful?Adjusting the objective aperture is an important step to obtain phase contrast in TEM. Compare the differences in objective aperture operation between diffraction contrast and phase contrast.What are Romanowsky stains? Name four of these. b: What are the components of a Romanowsky stain? b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.
- What are the limitations of UV Visible spectroscopy and fluorescence spectroscopy? Include reference link if possibleHow can a UV-Vis spectrophotometer be applicable in determining the absorbance/concentration of a colorless sample? Please answer in detail and clearly. Thank you so much!During staining, the first step is to dewax the slide. What is the purpose of the step? What is the effect of insufficient dewaxing?
