What is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handle

What is the purpose of fixing a smear? Mark all that apply: 1. To attach the bacteria to the slide 2. To cause the cells to shrink and become distorted 3. To kill the bacteria so they aren't harmed by the staining method 4. To break down the cell wall in order to make the cells accept stain 5. To kill the bacteria to make the slide safer to handle

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter7: Basic Clinical Microbiology

Section7.4: The Gram Stain

Problem 1CT

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Why is it important to limit the quantity of cells used to prepare a smear? Mark all that apply: 1. So that cells are not clumped and don't entrap stain creating erroneous results 2. So that the cells are spread out enough that cell morphology can be discerned 3. So that there are small groups of cells clumped together to make them visible 4. So that no contaminants are introduced onto the slide by being entrapped in clumps 5. So that the cells are spread out enough that the arrangement can be observed
A capsule stain is kind of: A Simple and Negative Stain B Differential and negative stain C Simple Stain D Differential stain E Negative Stain
What is the purpose of a bacterial smear? What is the purpose of a bacterial smear? It spreads the bacteria out into a single layer. It kills the bacteria. It helps the bacteria stick to the slide so they do not wash off during staining. All of the above. None of the above.
The benefits of the negative stain include: Mark all that apply: 1. No shrinkage or distortion of cells due to no heat fixing 2. Because the cells don't take up stain they don't get shrunk or distorted 3. One can more accurately determine the cell size and shape because there is no shrinkage or distortion of cells 4. Negatively charged dyes are safer to work with than positively charged dyes 5. One can more accurately determine cell size and shape because the cells stand out against the background
Crystal violet ( choose all that apply) A. Is not a good differential stain B. Will stain the cytoplasm of the cell walls C. Can be used to stain peptidoglycan on bacteria cell wall D. Can be used as an effective antimicrobial
The presence of a capsule around bacterial cells usually indi-cates their increased disease-causing potential and resistance todisinfection. Capsules are generally viewed by:(a) Spore staining(b) Scanning electron microscopy(c) Gram staining(d) Ziehl-Neelsen staining(e) Negative staining
If there are bacterial clumps visible in a drop of water on a glass slide, will this affect the staining of the bacteria? If there are bacterial clumps visible in a drop of water on a glass slide, will this affect the staining of the bacteria? No; the clumps will wash away later. Yes; the clumps will hide the individual bacteria. The clumps will not stain. Yes; the clumps of bacterial cells may retain the stains and lead to incorrect data being collected. SubmitRequest Answer
Abigail did not dry the top of her agar plate and stored it top up. Now she wants to count colonies but there is a smear over the entire plate. She was working with E. coli. Why does she have a smear?
How does smear preparation of cells from a liquid medium differ from preparation of cells from a solid medium? Mark all that apply: A smear made from liquid media will be thinner than a smear made from solid media A smear made from solid media doesn't have to be fixed but a smear from liquid media does A smear made from liquid media will be more thick than a smear made from solid Water must be added to a smear made from solid media but not liquid media A smear made from liquid media doesn't have to be fixed but a smear from solid media does Water must be added to a smear from liquid media but not from solid
Which of the following are true about a differential stain. There may be more than one answer! No chemicals are needed. More than one stain is used The acid fast stain is an example The Gram stain is an example.
observe the transparent bacteria under bright field microscope, we use a. Simple staining b. Gram staining c. Negative staining d Direct staining eAll the above
Which of the following describes the usefulness of a Gram stain. Select all that apply. a. A Gram stain can be used to check for bacteria at the site of a suspected infection or in certain body fluids, such as blood or urine. b. A Gram stain can be used to determine whether penicillin will be effective at treating the patient or whether a different antibiotic should be used. c. A Gram stain can determine whether the bacteria have a thick or thin layer of peptidoglycan in their cell wall.