Q: Is it possible to perform double or triple staining using fluorescent dyes? Explain
A: For both membrane and soluble proteins, fluorescent dyes are increasingly being utilized to monitor…
Q: Both crystal violet and safranin are basic stains and may be used to do simple stains on Gram…
A: Gram staining is the common procedure followed to distinguish the bacteria based on their cell wall.…
Q: is it essential that the primary stain and counterstain be of contrasting colors
A: Gram staining is one of the crucial type of differential straining .It divides bacteria into two…
Q: Distinguish between simple, differential, and structural stains,including their applications.
A: Staining is a method performed at a microscopic level to enhance the color in the sample. There are…
Q: Why is heat necessary in spore staining?
A: BASIC INFORMATION SPORULATION It is a process during which spores are formed. We can find it in…
Q: Principle and procedure for Mann’s methyl blue-eosin stain
A: Methyl blue stain Methylthioninium chloride or methyl blue is a stain use to highlight animal…
Q: In the Ziehl-Neelsen acid-fast stain, what is the mordant?
A: bacteria like Mycobacterium and Nocardia and the apicomplexan Cryptosporidium are not stained…
Q: What color would gram positive cells appear if ghr safranin staining step was left out of staining…
A: Gram staining is a technique used for differentiating the bacteria based on their cell wall…
Q: What is the purpose of acid-fast staining? What information can you gain from this procedure? Please…
A: The acid-fast stain is used to identify acid-fast organisms. Acid-fast organisms posess nearly…
Q: Differentiate between negative and positive staining, givingexamples.
A: These are the staining methods that are used to see bacteria under a microscope.
Q: Illustrated Diagram: Make an illustrated diagram of a stepwise procedure for the performance of…
A: Simple staining: Simple stain uses only one stain for staining bacterial cells. It gives basic…
Q: What is a differential stain? why is it used?
A: Differential stain is a type of stain use to identify organisms.
Q: Give the principle of acid fast staining and gram staining.
A: Microorganisms are extremely small in size and hence cannot be visualized directly. Specialized…
Q: What is the principle of Giemsa staining and components of Giemsa stain?
A: The cells are the basic structural and fundamental unit of the living system. It consists of…
Q: What is the difference between simple stains anddifferential stains?
A: Simple stains : It generally make all of the organisms in a sample appear to be the same color even…
Q: Defi ne dyes and describe the basic chemistry behind the process of staining.
A: Staining is a method by which contrast in a sample is enhanced usually at the microscopic level.…
Q: Describe how simple staining and fluorescence staining are similar and how they are different. What…
A: Microscopy is a technical field for the use of microscopes to visualize objects and locations of…
Q: What are the Types of Differential Stains? explain with an example.
A: Bacteria are prokaryotes that are devoid of any membrane-bound organelles. They are differentiated…
Q: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
A: What stain technique is used here? What is the primary stain, mordant, decolorizing agent, and…
Q: Is acid fast staining differential stain , if yes, what are the 2 types of stain used
A: Differential Staining is defined as a staining process which utilizes more than one chemical stain.…
Q: explain the spotting effect of ethyl alcohol, acetone and coconut oil when place into the paper
A: When ethyl alcohol is spotted onto paper, it wets the paper and after some time this spot will…
Q: Stains Primary Stain Mordant Destain Secondary Stain Diagram of Stain Simple Stain N/A N/A N/A Gram…
A: This question is regarding different staining techniques of microorganisms.
Q: Protocol for Mann's methyl blue eosin stain technique
A: The anterior pituitary gland and viral appearance are stained with a polychromatic solution of…
Q: write the protocol for mann's methyl blue-eosin staining, including their reagents, materials,…
A: Reagents: Alkaline alcohol reagent –100ml of absolute Ethanol or Methanol containing 0.2ml 0.1N…
Q: What is safe view dye for? Compare it with Ethidium Bromide.
A: Safe view dye is a nucleic acid stain which is used for the detection of double-stranded DNA,…
Q: Differentiate the “hot method” and “cold method” of acid-fast staining
A: Introduction Staining is a technique for enhancing contrast in material, usually on a microscopic…
Q: Differential stains function to
A: The widely used staining method in the microbiology is the differential staining that are gram…
Q: Give the color reaction in Wright’s stain and the function of these
A: Wright's stain is a polychromatic stain consisting of a mixture of eosin and methylene Blue. It is a…
Q: Differentiate between simple and differential stains
A: We’ll answer the first question since the exact one wasn’t specified. Please submit a new question…
Q: Select all of the following that represent examples of positive stains. Check All That Apply…
A: Staining is the common laboratory procedure which can be specific and general according to the use.…
Q: Why is it necessary to use contrasting stains while staining
A: Introduction:- Cell staining is a technique that allows you to see cells and cell components more…
Q: Positive Staining Negative staining
A: Staining Procedure Dye Charge Of The Chromophore/ Colored Ion Charge Of The Target Cellular…
Q: The primary stain used in the endospore stain is: A. Carbolfuchsin Stain B. Methylene Blue C.…
A: To differentiate between the vegetative cells and the endospores, a differential staining technique…
Q: What is a crystal violet stain and its purpose?
A: Crystal violet is that the name given to the cluster of similar organic compounds that are used as…
Q: Outline the main differential stains and how they are used.
A: The detection and observation of cells and microorganisms in their natural state under microscope is…
Q: Define dyes and describe the basic chemistry behind the processof staining.
A: Detection and observation of cells and microorganisms in their natural state under microscope is…
Q: Write the instructions, reagents, principles and the procedures for the following. 1. Periodic acid…
A: (AS PER OUR GUIDELINES WE ARE PROVIDING YOU ANSWER OF 1ST TWO QUESTIONS. YOU CAN POST OTHER…
Q: What is the primary stain for the Ziehl-Neelson acid fast stain? What color is this stain? What…
A: In microbiology, the technique that is used to enhance the contrast in samples, at the microscopic…
Q: When performing a negative stain, how does it work and why does it only stain the background?
A: * Negative stain is practice of applying heavy metal salt stain to sample for observation hence…
Q: In staining, why is there a need for contrasting stains?
A: Answer
Q: How is staining techniques useful in the clinical settings
A: Staining: A sample at the microscopic level can easily be visualized and distinguished from the…
Q: For What purpose is Giemsa stain used for?
A: Staining is a technique used in microscopy. Staining is mainly used in biology to highlight the…
Q: What is the primary stain in the Kinyoun acid-fast stain? How does this differ from the primary…
A: Need to explain the primary stain in the Kinyoun acid-fast stain.
Q: Briefly describe the uses of Special stains.
A: Routine stain: It refers to the hematoxylin and eosin (H&E) staining that is used routinely to…
Q: Name two fixatives used in staining?
A: Histology is the study of ultrastructure of tissues using thin sectioning techniques like microtomy,…
Q: What is the difference between simple and differential stains? Give an example of a differential…
A: Bacteria are microorganisms that are colorless for most of the part and in order to study their…
Q: Specific Dyes used in flagella stain: A wet-mount procedure (Ryu method) Dried-smear preparation…
A: Flagella is a hair-like structure that is found in bacteria, archaea, and Eukaryota. It allows the…
Explain the mechanism of staining for each of the stains used.
- Iodine
- Methyl green
- Nigrosin
Step by step
Solved in 3 steps
- Select all of the following that represent examples of positive stains. A) nigrosin B) methylene blue C) crystal violet D) India ink E) safraninDifferentiate the “hot method” and “cold method” of acid-fast stainingThe acid fast technique is also known as: A. Ziehl-Neelsen stain B. Auramine-rhodamine stain C. Gerrard-Lampard Stain D. Kinyoun method
- Create paragraphs to differentiate among the following terms: positive stain, negative stain, simple stain, and differential stain. please provide minimum of 400 wordsWrite down the materials, reagents, Principle, procedure and results for Mann’s methyl blue-eosin stainWrite down the materials, reagents, Principle, procedure and results for Ziehl-Neelsen stain
- In four steps, describe the simple staining procedure.Write the protocol for lactophenol blue stain method indicating; materials, reagents, Principle, procedure and results.You mistakenly confused the primary stain and counter stain with crystal violet, how does you misture now appear when viewed with oil immersion?
