Bubble boy disease is a deficiency in adenosine deaminase that results in the accumulation of DADP. As a resu O The specificity of ribonucleotide reductase becomes unregulated and only pyrimidines are synthesized O Allosteric binding of excess DADP cause the R1 dimer to separate into monomers O The reaction equilibrium shifts and ribonucleotide reductase starts synthesizing ribonucleotides O The overall activity of ribonucleotide reductase is inhibited The tyrosil-radical site is disrupted by binding of excess DADP
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- One of the important features of enzymes is that they stabilize transition state of the reaction they catalyze. What is directly responsible for Trypsin and Chymotrypsin’s stabilization of the transition state? (select one) a) The F helix b) Valine c) The specificity pocket d) Serine e) The Oxyanion holeDraw the strucures of Adenosine Triphosphate (ATP) and Cytidine Triphosphate and Show how they combine to form the polynuleotide (i.e. only the end product). Show at any one of these structures where the glycosidic bond occursTissues that are unable to produce nucleotides de novo depend on ____________________ to maintain an adequate supply of adenine nucleotides. (A) the redox reactions carried out by ribonucleotide reductase (B) the action of PRPP amidotransferase(C) uptake of ATP through dietary intake(D) salvaging hypoxanthine using HGPRT (E) the action of xanthine oxidase
- Starting from glutamine, glycine, aspartate, N 10-formyi-THF, how many ATP- equivalents are required for purine nucleotide synthesis? 2 8 4 6a. Why is it important to eat food containing antioxidants? Write at leasttwo reactions to prove the answer. b. Write in four ways the following nucleotide sequence: ATGCA. c. Explain i. Hoogsteen pairing & ii. Hyperchromic effectiii. Epimers Iv. Mutarotaton v. Aldose vi. Anomers vii. MutarotationThe following is diagram of a generalized tetranucleotide. Carbons exist at corners on the shapes and phosphate groups are filled circles A. Suppose that one of the precursors for the tetranucleotide in the diagram was a 32P-labeled guanine nucleoside triphosphate (the innermost phosphate containing the radioactive phosphorus). Where is the radioactive phosphorus atom (i.e., one of the solid black balls) as it exists in the tetranucleotide B. If spleen diesterase (which induces breaks between the phosphate and the 5’ carbon) is used to digest the pictured tetranucleotide, state which base(s) among the breakdown products will be expected to be attached to the 32P.
- where is a reducing end in this image? I initially thought it'd be on the middle oxygen connecting the lactose molecules since it's the only reducing sugar, but that was incorrect for some reason.In the deoxy state of HB, which of the following does not occur? a) An inter-chain salt bridge between Histidine 146 and Lys 40 b) An intra-chain salt bridge between Val 98 and Tyr 145. c) An intra-chain salt bridge between Histidine 146 and Aspartate 94 side chains d) A peptide bond between Tyr 145 and His 146.Ribonuclease A cannot catalyze the hydrolysis of DNA. which of the following statements explains it. a. Ribonuclease requires two active site histidines to be active but the nucleobase of DNA will form hydrogen bonds with these histidines and block their acid-base catalysis. b. DNAs have thymidine that is more stable than the uracil in RNAs c. DNAs are double-stranded and the nucleobases are protected while RNAs are single stranded d. DNAs does not have hydroxyl group at 2' position of the sugar ring to support the catalysis
- Which of the following enzyme:function pairs is/are correct? a.primase:forms RNA primer b.ligase:join Okazaki fragments c.gyrase:relax supercoils d.DNA POI ll:removes RNA primersMutations are caused by chemical and physical phenomena. Indicate the type of mutation that each of the following reactions or molecules might cause: a. ROS b. intercalating agents c. a small alkylating agent d. a large alkylating agent e. nitrous acid2) A cell wants to synthesize the a(1→4) dimer from two glucose molecules. Show the mechanism.