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- DILUTION COLONY COUNT CFU/mL 1:106 155000 1:107 15500 1:108 1550 1:109 155 Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it. Assuming you plated the same volume on each plate, how many colonies would you expect to find on the 10^-2 plate? Include units and use OCD formula.In the spread plate method, why is the volume plated usually limited to not more than 0.1 mL?
- You are testing unpasteurized milk for the presence of bacterial contamination. Starting from the undiluted milk, you do serial dilutions, and plate 0.5 ml of 4th dilution on agar. If you counted 78 CFUs from the 4th plate, how many bacteria/ml do you expect? (show solution) Do you think standard plate counts are very accurate? Why or why not?Test tube Ascorbic acid stock (20 μg/mL) Citrate buffer Final Concentrations 1 0 μL 1000 μL 0.0 μg/mL 2 125 μL 875 μL 2.5 μg/mL 3 250 μL 750 μL 5.0 μg/mL 4 500 μL 500 μL 10.0 μg/mL 5 750 μL 250 μL 15.0 μg/mL 6 1000 μL 0 μL 20.0 μg/mL 7 1 mL of the fresh orange juice (20x diluted) 8 1 mL of the heated orange juice (20x diluted) 9 1 mL of the pre-citrus urine sample (5x diluted) 10 1 mL of the post-citrus urine sample (5x diluted) Plot a graph in which y-axis represents for absorbance and x-axis represents for concentration From graph, y=mx + c Calculate the vitamin C concentrations of samples by the equation with consideration of dilution factors. 1. StandardCurve Sample (μg) Absorbance (520 nm) 0.0 0.621 2.5…1200 ml of iv fluid must be administered to the patient over an 8 hour period. If the calibration rate is set a 8 drops/ ml, how many drops per minute will be administered to the patient?
- How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.Observe the following Plate counts and then determine the correct number of CFU/ml Plate 1 = 564 colonies at 10^-5 dilution Plate 2 = 422 colonies at 10^-6 dilution Plate 3 = 317 colonies at 10^-7 dilution Plate 4 = 93 colonies at 10^-8 dilution 93 x 10^10 CFU/ml 9.3 x 10^-9 CFU/ml 93 x 10^9 CFU/ml 93x 10^8 CFÜ/ml 93x 10^-8 CFU/ml asap pleaseHow do you calculate the initial concentration of a sample? How would you make a 1:10 dilution of a broth culture if the final volume of the dilution is 10 ml? How much broth, and how much diluent, would you add? If you made a series of tenfold dilutions, exactly the same way as you described in question 1, what would be the dilution factor in the fifth tube? The following are errors that people commonly make when they perform serial dilutions. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. You intend to add 0.9 ml of diluent to each tube and 0.1 ml of culture. Instead, you add 0.5 ml of diluent to each tube and 0.1 ml of culture to the first tube. Then, you make a serial dilution of 0.1 ml into and from each tube as described. You prepare 0.9 ml of diluents in each tube. You add 0.1 ml of culture (from the overnight culture provided) to every tube. You add 0.9 ml of diluent to each tube. You add 0.1 ml of…
- Explain why the following steps are essential during subculturing:a. Flaming the inoculating instrument prior to and after each inoculation.b. Holding the test tube caps in the hand as illustrated in Figure 1.1 on page 24.c. Cooling the inoculating instrument prior to obtaining the inoculum.d. Flaming the neck of the tubes immediately after uncapping and beforerecappingAn original sample of water containing 4.00 X 106 CFU/mL was diluted by 4 successive 1/10 dilutions. After incubation, 200 colonies were found growing on the plate. How many mLs from the last dilution tube were plated out?The plate with 45 colonies was inocculated with 100 µL Volume of a 10^-8 Dilution. Given the formula OCD = CFU/V x D, what as the Original Colony Density?