In using the centrifuge machine, how will this be remedied if there are only 3 separate liquid mixtures, in order to separate their respective precipitates
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In using the centrifuge machine, how will this be remedied if there are only 3 separate liquid mixtures, in order to separate their respective precipitates
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- In the experiment, imidazole is used to elute mCherry from the column. Instead of using imidazole, can you suggest another method to elute mCherry from the column?How do you calculate the initial concentration of a sample? How would you make a 1:10 dilution of a broth culture if the final volume of the dilution is 10 ml? How much broth, and how much diluent, would you add? If you made a series of tenfold dilutions, exactly the same way as you described in question 1, what would be the dilution factor in the fifth tube? The following are errors that people commonly make when they perform serial dilutions. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. You intend to add 0.9 ml of diluent to each tube and 0.1 ml of culture. Instead, you add 0.5 ml of diluent to each tube and 0.1 ml of culture to the first tube. Then, you make a serial dilution of 0.1 ml into and from each tube as described. You prepare 0.9 ml of diluents in each tube. You add 0.1 ml of culture (from the overnight culture provided) to every tube. You add 0.9 ml of diluent to each tube. You add 0.1 ml of…How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.
- Explain this example of the following water quality serial dilution. Note the dilution factors and how you would calculate #’s from the incubated plates. ……A serial dilution yielded TNTC in the 1:1 plate and the 1:10 plate, 50 cfu’s in the 1:100 plate and 3 cfu’s in the 1:1000 plate . What is the concentration (CFU)/ml of the raw sample? Is water acceptable for potable or Recreational use? Explain. When do you use filtration rather than serial dilutionRefer to the chromatograph (Figure 1) below and answer the questions that follow: What is the volumetric flow rate of the column? What type of compound is likely to be found in peak B? Explain. What possible buffer changes could the researcher have applied at the 30ml mark? Explain. How would one avoid cross-contamination when using this type of purificationIn the spread plate method, why is the volume plated usually limited to not more than 0.1 mL?
- What volume of the saline solution must be administered to the patient in order to deliver 7.7 g of NaClIn your opinion and based on your knowledge, what is the most important component that will be tested using the reagent test strips?Explain why it is important to follow proper precautions and use aseptic technique when working with clinical samples. Why do you think that samples from urine should be tested within 2 hours of collection, or if not possible, that the sample should be refrigerated?
- A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it. Assuming you plated the same volume on each plate, how many colonies would you expect to find on the 10^-2 plate? Include units and use OCD formula.A stock solution was diluted in two steps. In a first step, 0.5 ml was diluted with 9.6 mL buffer. In a second step, 0.5 mL from the first step was diluted with 9.6 mL of buffer. The concentration of the mixture of the second step was determined to be 0.24 mg/mL. What was the concentration of the stock solution?Explain why the following step are necessary during subculturing: Flaming the neck of tubes/bottles after capping and before re-capping?