2. a) Calculate the CFU present in 100 mL water sample and b) dilution factor in Tube 5. Consider the following dilution scheme: 5 ml 1 ml 0.5 ml 1 ml 0.1 ml 0.9 ml Water sample 9 ml 4.5 ml 100 ul. 100 ml 45 ml 19 ml 100 ul 100 ul 100 ul 100 pl No. of colonies: TNTC 425 58 12 1 3.
Q: 4. acetic acid on the ammonium sulfate precipitation of albumin 5. presence of precipitate during…
A: Molecular techniques are important tools that help in filtration, isolation, and identification of…
Q: 11. ● Possible methods for the quantitative determination of sodium bicarbonate for injection are:…
A: We'll answer the first question since the exact one wasn't specified. Please submit a new question…
Q: ow many mL of 3% sodium chloride will be needed to complete the following compound? Sodium…
A: To calculate the ml of 3% sodium chloride -' Mass of Sodium bicarbonate - 4.2 gm = 4200 mg Sodium…
Q: J A positive result for Fohl's test is the formation of a black precipitate in the form of lead. O A…
A: Fohl's Tests - This test is performed to detect the presence of amino acids containing sulfur. The…
Q: What are the color code of the following Blood Additives/Anticoagulants? 1. None 2. Clot activator…
A: Phlebotomy refers to the process of puncturing the vein with a cannula to collect blood. Blood…
Q: FFF PRELAB: 1. Complete the following conversions: 1 uL 100 µL 250 pL = a. mL mL mL d. 1.5 mL 0.06…
A: Measuring volumes plays an important role in the field of biology and chemistry to perform an…
Q: The table contain results of using highly accurate P-1000 micropipette. The res Target volume 1000…
A: A two-position plunger with friction "stops" is standard on most micropipettes. The loudness is…
Q: At 2pm you received a doctors order that you have to Infuse 1000 mL of D5W in 8 hours to Mr. Dela…
A: 1000 ml to be infuse in 8 hours
Q: A plate has 72 colonies with a total dilution factor of 10-7, 0.1 ml was pipetted onto the plate,…
A: Colony forming unit (CFU) is a unit that tells us the amount of viable cells present in the given…
Q: 9. The physician orders a PCA drip of morphine sulfate 200 mg in 1,000 mL of D5W to be infused at a…
A: There are main 3 primary methods for calculating medication dosages. All the drug calculations will…
Q: You are going to administer an oral suspension of Vistaril. The suspension label reads that there is…
A: Formula used- 1 ounces = 30ml 1tablespoon=15ml
Q: 1. One mL of sludge was added to a 99 mL dilution blank. Three 1/10 dilutions were then made. From…
A: CFU/ml represents the number of colony-forming units in the sample. It gives us the estimation of…
Q: m depth in d
A: Duke’s bleeding time test can be defined as 3-4mm deep pricking of the skin through a lancet or…
Q: 1- Are the following changes in conditions likely to increase or decrease retention time? a. faster…
A: "Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: Given that the vol of blood taken is 20uL, and the vol of the diluting fluid is 5.0ml. Calculate the…
A: A hemoglobin test measures the amount of hemoglobin in your blood. Hemoglobin is a protein in your…
Q: Procedure: 1. Prepare 6 test tubes and place 1 ml saliva in each. 2. Add Iml water to tube 1,2 ml to…
A: Iodine test: This is specific test for the identification of starch in the given sample. If starch…
Q: What are the chemical reaction of the following Blood Additives/Anticoagulants? 1. None 2. Clot…
A: medicines that helps to prevent blood clot are known as anticoagulants they are also known as blood…
Q: Explain the reason for each of the following rules regarding micropipettes. a) Always set the…
A: A micropipette is a laboratory Instrument used to transfer precise and accurate volumes of…
Q: Construct a table showing the composition, advantage and disadvantage of these Platelet Diluting…
A: The fluid used as diluents must be isotonic, too have a certain high gravitational pull barrier cell…
Q: How many iters of half normal saline IV soktion with a concentration of 0.45% (m/v) can be prepared…
A: Dilution is the process of decreasing the concentration of solute in a solution generally by mixing…
Q: A. Discuss the procedure and give the advantages and limitations of: 1. Kato - Katz Technique 2.…
A: Several techniques can be employed in the laboratory to find out or diagnose the specific disease or…
Q: Calculate the number of PFU/ml in the stock bottle. 1.0 ml 1.0 ml 1.0 ml # 1 # 2 # 3 99 ml 99 ml 99…
A: PFU is also called as plaque forming unit. It is a bacteriophage capable of productively infecting a…
Q: Calculate the CFU present in 100 mL water sample and b) dilution factor in Tube 5.
A: Answer a - CFU present in 100ml solution=58×104 CFU/ml= 580,000 CFU/ml CFU present in 100ml sample=…
Q: Identify whether the statements are TRUE or FALSE 1. You should use P-20 micropipettes to transfer…
A: Pour plate is a technique , in microbiology in which the quantity of the colony-forming units of…
Q: A patient is receiving an IV solution at a rate of 125ML / hr. How much solution will she receive in…
A: Given, the rate of solution which is given to the patient = 125ml/hr. Which means that in 1 hour,…
Q: 1. Why is necessary to remove fat and tendons from the heart sample? 2. Why is necessary to…
A: Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you…
A: Cfu refers to the colony forming unit which is formed through continuous replication of a single…
Q: Give 3 uses of calculations of concentrations of solutions to the medical settting
A: The concentration of the solution is the measure of a solute that is dissolved in a particular…
Q: dispensed
A: Drug calculation uses the conversion of units such as kilograms to grams and liters to mL. Accurate…
Q: You are to prepare capsules that contain 0.125 g of a drug. You have four partial containers of…
A: The accuracy of the weighing is hundredth of a gram meaning the accuracy is 0.01 g. This scale can…
Q: 3. Imagine that you inoculated 5 tubes of BGLB for each of three dilutions, and found a combination…
A: MPN It is the estimation of number of organism group in a unite volume of sample.
Q: During reflux, what is the most suitable heater setting? How long the liquid needs to be boiled at…
A: Reflux involves the condensation of vapor and returns the condensate to the solution. It is one of…
Q: A test procedure calls for 5 mL of undiluted specimen. 2 mL of a 1:50 dilution is used. The answer…
A: Dilution is a procedure of reducing the concentration of a sample. This is usually performed by…
Q: 6, If Mr. Strong is admitted to the hospital and administered Buprenex, how much will you draw up in…
A: Dose : It is defined as the amount of medicines that has to be taken orally or intravenously is…
Q: a. How much diluent is added to reconstitute the vial? b. What is the concentration of the…
A: Drug ordered is ceftriaxone Ordered dose = 500mg Dose available = ceftriaxone 10gm vial Frequency…
Q: Order: ABC 240 mcg po. Stock: ABC 160 mcg po scored tablets. How many tablets will the patient take…
A: The dosage calculation for tablets is very crucial in nursing and is important information that…
Q: Determine the CFU/ml for each of the following. 1. Dilution Plate #1 | Plate #2 | CFU/ml 10-2 110 |…
A: Specific bacteria can be isolated from mixed culture by using various plating techniques like the…
Q: Penicillin V Potassium Oral Solution 125 mg/5 ml. Disp. ---ml. Sig. 5 ml q 6 h ATC × 10 days. How…
A: The dose is the quantity of the drug taken at a single sitting. The dosage refers to the volume and…
Q: Which of the following is not a therapeutic agent used before death? A. Cumadine B.…
A: Here we have to find out which is not a therapeutic agent used before death. Means which one is a…
Q: Make the following prescription isotonic with lacrimal fluid: 0.06 g Ephedrine sulfate (E = 0.23)…
A: Asked : Volume of sterilized distilled water (in milliliters) that should be added to the…
Q: A doctor had ordered 1 floz 4% medication syrup bid X 5d. How many floz are required in total? How…
A: Introduction:- bid means twice daily or 12 hourly. Each dose is 1 floz and two times a day is to be…
Q: Vol to transfer: ml ml ml ml Overnight culture
A: Serial dilution is a method used to decrease the concentration of a sample sequentially . During…
Q: 1 mL of supernatant is required for a procedure. The final colored solution proves to be too high to…
A: Introduction: The concentration of a solution is an estimation of the amount of solute that is…
Q: I. Give one(1) alternative testing method for one (1) of the ten parameters tested in a urine…
A: Introduction A dipstick test or basic urine strip test is a standard diagnosis of various components…
Q: How many hours will Dexmedetomidine 200mcg in 50ml last/finish if the doctor's order is 0.05…
A: In the healthcare setting to administer the drug to the patient is the duty of the nurse. So it is…
Q: n routine stool examination, what is the "Scotch Tape" Method? Guide: a.) What the procedure is…
A: In hospitals or healthcare centers, a stool test is done to diagnose any condition that affects the…
Q: add 2.75 mL out of 5.0 mL of an undiluted sample to 125.25 mL. ad, you add all 5.0 mL to the 125.25…
A: We should we the formula to solve this question: Dilution factor = Final volume of the…
Q: How many mL of 3% sodium chloride will be needed to complete the following compound? Sodium…
A: A drug is a chemical compound that is administered to the body for the purpose of improving health.…
Q: Blood added with 25 ml distilled water + submerged in water bath, added 1-2 drops of 10% acetic acid…
A: Organic molecules are considered to be complex molecules that contain carbon and are mainly…
Trending now
This is a popular solution!
Learn your way
Includes step-by-step video
Step by step
Solved in 2 steps
- A sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it. Assuming you plated the same volume on each plate, how many colonies would you expect to find on the 10^-2 plate? Include units and use OCD formula.DILUTION COLONY COUNT CFU/mL 1:106 155000 1:107 15500 1:108 1550 1:109 155 Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?How would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.
- Test tube Ascorbic acid stock (20 μg/mL) Citrate buffer Final Concentrations 1 0 μL 1000 μL 0.0 μg/mL 2 125 μL 875 μL 2.5 μg/mL 3 250 μL 750 μL 5.0 μg/mL 4 500 μL 500 μL 10.0 μg/mL 5 750 μL 250 μL 15.0 μg/mL 6 1000 μL 0 μL 20.0 μg/mL 7 1 mL of the fresh orange juice (20x diluted) 8 1 mL of the heated orange juice (20x diluted) 9 1 mL of the pre-citrus urine sample (5x diluted) 10 1 mL of the post-citrus urine sample (5x diluted) Plot a graph in which y-axis represents for absorbance and x-axis represents for concentration From graph, y=mx + c Calculate the vitamin C concentrations of samples by the equation with consideration of dilution factors. 1. StandardCurve Sample (μg) Absorbance (520 nm) 0.0 0.621 2.5…The plate with 45 colonies was inocculated with 100 µL Volume of a 10^-8 Dilution. Given the formula OCD = CFU/V x D, what as the Original Colony Density?You are testing unpasteurized milk for the presence of bacterial contamination. Starting from the undiluted milk, you do serial dilutions, and plate 0.5 ml of 4th dilution on agar. If you counted 78 CFUs from the 4th plate, how many bacteria/ml do you expect? (show solution) Do you think standard plate counts are very accurate? Why or why not?
- How do you calculate the initial concentration of a sample? How would you make a 1:10 dilution of a broth culture if the final volume of the dilution is 10 ml? How much broth, and how much diluent, would you add? If you made a series of tenfold dilutions, exactly the same way as you described in question 1, what would be the dilution factor in the fifth tube? The following are errors that people commonly make when they perform serial dilutions. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. You intend to add 0.9 ml of diluent to each tube and 0.1 ml of culture. Instead, you add 0.5 ml of diluent to each tube and 0.1 ml of culture to the first tube. Then, you make a serial dilution of 0.1 ml into and from each tube as described. You prepare 0.9 ml of diluents in each tube. You add 0.1 ml of culture (from the overnight culture provided) to every tube. You add 0.9 ml of diluent to each tube. You add 0.1 ml of…Using a P-1000 micropipette, you transfer 800 microliter of ddH2O to a beaker and measure its weight. It turns out to be only 780 milligram; what is the percent error of this micropipette?1200 ml of iv fluid must be administered to the patient over an 8 hour period. If the calibration rate is set a 8 drops/ ml, how many drops per minute will be administered to the patient?
- In sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperObserve the following Plate counts and then determine the correct number of CFU/ml Plate 1 = 564 colonies at 10^-5 dilution Plate 2 = 422 colonies at 10^-6 dilution Plate 3 = 317 colonies at 10^-7 dilution Plate 4 = 93 colonies at 10^-8 dilution 93 x 10^10 CFU/ml 9.3 x 10^-9 CFU/ml 93 x 10^9 CFU/ml 93x 10^8 CFÜ/ml 93x 10^-8 CFU/ml asap pleaseI do not understand the results of this experiment, explain what the results of this experiment are and the purpose Table 2 Absorbance vs CoCl2 concentration Test Tube Number Cobalt Chloride Concentration (mol/mL) Absorbance at 510 nm 1 0.000 0.000 2 0.009 0.024 3 0.018 0.055 4 0.027 0.085 5 6 0.036 0.045 0.112 0.138 7 (Unknown) 0.088