Calculate the following DNA concentrations and determine the purity of the samples: DNA Dilution Dilution A260 Concentration A260/280 Pure Explain Sample factor DNA? 1 1 ul in a total 10/1 = 1.98 1.56 volume of 10 10ul 2 0.5 ul in a 20 2.5 1.82 total volume of 10ul 3 5 ul in a total 0.86 1.95 volume of 25 ul Show your calculations (for samples 1-3 above) below: DNA conc = (A260 reading) x (absorbance factor) x (dilution factor)| If you have 20 ul of each DNA sample above (samples 1-3), what is the total DNA yield (concentration)? Show your calculations below:

Calculate the following DNA concentrations and determine the purity of the samples: DNA Dilution Dilution A260 Concentration A260/280 Pure Explain Sample factor DNA? 1 1 ul in a total 10/1 = 1.98 1.56 volume of 10 10ul 2 0.5 ul in a 20 2.5 1.82 total volume of 10ul 3 5 ul in a total 0.86 1.95 volume of 25 ul Show your calculations (for samples 1-3 above) below: DNA conc = (A260 reading) x (absorbance factor) x (dilution factor)| If you have 20 ul of each DNA sample above (samples 1-3), what is the total DNA yield (concentration)? Show your calculations below:

General, Organic, and Biological Chemistry

7th Edition

ISBN:9781285853918

Author:H. Stephen Stoker

Publisher:H. Stephen Stoker

Chapter22: Nucleic Acids

Section: Chapter Questions

Problem 22.84EP

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