Why is it important that the standard curve you create in biological analyses with spectrophotometry is measured using specialty cuvettes?

Q: If a researcher forgot to wipe the outside a sample cuvette to make it free of drops, fingerprints,…

A: Absorbance is the amount of light absorbed by chemical species present in the sample. It is measured…

Q: p between absorbance (A) and transmittance (T) for the basic spectrophotometer technique.

A: A method to measure how much a chemical substance absorbs light by measuring the intensity of light…

Q: Why is the wavelength of the light that is released in spectrophotometric analysis longer than the…

A: Some molecules absorb light at a particular wavelength and emit light in higher wavelength and this…

Q: How can a UV-Vis spectrophotometer be applicable in determining the absorbance/concentration of a…

A: A spectrophotometer is an instrument that is used to measure the intensity of light absorbed by the…

Q: Explain why specimens should be taken aseptically and processed quickly to ensure accurate results,…

A: Microbiology is the study of microscopic organisms, such as bacteria, viruses, Achaea, fungi and…

Q: Can you perform spectrophotometric analysis without the following? a. Bradford dye b. Molecular…

A: Protein is a macronutrient that is vital for building bulk. It is usually found in creature items,…

Q: What are the differences between scanner, low power objective, high power objective and oil…

A:

Q: Calculate the size of an organism that occupies 26 um spaces under the scanner, LPO, and HPO using…

A: An ocular micrometer is used to measure the size of objects. The stage micrometer is used to…

Q: Differentiate spectroscopy, spectrometry, and spectrophotometry

A: The study of the interaction of matter with electromagnetic radiation as a function of the…

Q: Why is depth of field important in studying biological structures? How can it affect your ability to…

A: Microscopy is a technique that is used to visualize objects that are not visible to the naked eyes.…

Q: Why is a blank used in the spectrophotometer before the actual pigment is tested?

A: spectrophotometer is a device used in spectral analytical techniques like UV spectroscopy , in which…

Q: How do you identify which objective lens are you using to view your sample?

A: microscope objectives feature a range of magnifications from 1.25x to 150x. This is the first…

Q: Why is it that a warm cuvette does not lose any significant heat during the absorbance measurement…

A: An absorption spectrophotometer is defined as a device that is used to calculate the absorbed light…

Q: How can you tell if the current or the gel electrophoresis is already running and what are the…

A: Gel electrophoresis is the process where DNA, RNA, and protein fragments get separated depending on…

Q: Three-dimensional images of specimens can be produced using z stacks obtained from confocal…

A: Microscopy is a technique that is used to visualize small-sized objects that are not used to the…

Q: At what wavelengths should the absorbances be read to examine the purity of a DNA sample with a…

A: A spectrophotometer is a tool that measures the number of photons (light energy) absorbed after…

Q: what is the importance of getting rf value in chromotography tools?

A: Chromatography: Chromatography is an analytical technique in which compounds in a mixture are…

Q: What is a Spectrogram

A: Spectrogram is a visual way of viewing the spectrum of frequencies of signal that varies with time.

Q: Can colorless samples be analyzed using a UV-Vis spectrophotometer? Yes or No or Maybe?

A: UV-Vis Spectroscopy  It is defined as a quantitative technique that is used to identify the…

Q: What is the total magnification of a specimen viewed with a 10x ocular lens and a 45x objective…

A: The correct ans is 450X. The total magnification of specimen viewed with a 10x ocular lens and 45x…

Q: Is it possible to determine Km and Vmax without using excel?

A: Vmax  It refers to the maximum reaction velocity at which all enzymes become saturated with…

Q: Enumerate the different kinds of blanks used in spectrophotometry

A: Introduction :  Spectrophotometry is a method used to analyse molecules quantitatively based on how…

Q: The specimen that you are trying to find is very small and you cannot see it with scanning power.…

A: Microscopy is a field which involves using a microscope to view those specimens in enlarged size…

Q: principle of basic spectrophotometer

A: Spectrophotometer is an advanced instument which measures the amount of light absorbed by a sample.…

Q: Here are the materials and method for the basic spectrophotometer experiment Materials: 1. Paper…

A: A spectrophotometer is a device used to measure the spectrum of visible light. The wavelength and…

Q: In confocal microscopy, what is the theoretical resolving power of the objective used (63X, NA 1.3,…

A: Resolving power signifies the smallest distance between two separate points of an object, when…

Q: Why does one see methyl orange as orange in color? What blank would be used to standardize this…

A: 1. Methyl orange is a pH indicator frequently used in titration because of its clear and distinct…

Q: spectrophotometer.

A: Definition:  It is defined as the branch of electromagnetic spectroscopy which is concerned with…

Q: When using a light microscope, objective lenses can be found to have a magnification of all of the…

A: Light microscopes are devices that are used to magnify small objects by using visible light.…

Q: How can one obtain 2,000× magnification with a 100× objective?

A: Magnification means how many times the image is larger than the object.

Q: In light microscopy, when magnification is increased, the lens focuses closer to the sample.  This…

A: Microscope helps to observe the organisms or objects that cannot be seen by our naked eye because…

Q: When using the 10x lens to view a specimen, what is the total magnification of the specimen to the…

A: When using the 10x lens to view a specimen, what is the total magnification of the specimen to the…

Q: What do you mean by Auxanometer?

A: The process of plant development can be easily studied by the activity of the meristem. The…

Q: What is the importance of sllid width in fluorescence spectroscopy

A: Fluorescent microscopy is often used to image specific features of small specimens such as microbes.

Q: Give two applications of Spectrophotometric analysis and briefly explain each

A: The spectrophotometer is an instrument that uses an electromagnetic spectrum. This instrument is…

Q: Assume your field of view under a 3X objective is 6mm across. What is the field of view in mm under…

A: Since you have asked multiple questions, we will solve the first question for you. If you want any…

Q: Describe how you would use a compound light microscope with the 4 objective lenses to view a…

A: A compound microscope is a microscope with more than one lens and its light source. In this type of…

Q: Explain the difference between a transmission electronmicrograph (TEM) and a scanning electron…

A: A microscope is a laboratory instrument that is used to examine objects that are too small for the…

Q: Total magnification of a specimen using the high objective and the ocular lens is

A: B) 40Ox 400 x is the typical magnification of an ocular lens.

Q: Which spectrophotometer mode should you use to calibrate the instrument and which mode should you…

A: Spectrophotometer is an optical instrument that is used to measure the light intensity relative to…

Q: What is the differences between ocular magnification and objective magnification?

A: Introduction  A microscope's objective lens is the one near the sample at the bottom. It's an…

Q: When a sample presents some matrix interference, which of the ff. are carried out? Dilute sample to…

A: shift in analytical results caused by one or more specific constituents in the matrix, generally…

Q: In a tabular form, enumerate the different components of a spectrophotometer and explain its…

A: A spectrophotometer is an analytical equipment that is used to measure the absorbance of light by…

Q: Why is it necessary to cut thin sections of the tissue sample from a specimen using a microtome?

A: Microtomes are specialized cutting instruments that produce very thin slices of tissues so that the…

Q: How do you know the range in microliters within which a given micropipette can accurately measure?

A: A micropipette is a device used to transfer small volume of samples . It is a vital part in…

Q: Discuss the types of interferences in atomic absorption spectrophotometry.

A: Discuss the types of interferences in atomic absorption spectrophotometry. Introduction:  Spectral…

Q: what is experimental error that may occur when reading spectrophotmeter

A: Spectrophotometry is a technique for determining how much light a chemical substance receives by…

Q: Explain any 2 advantages the fully automated analyser has over the spectrophotometer.

A: An automated analyzer is an instrument that analyses various chemical or biological samples. The…

Scientific Notation

We have to deal with quantities that are extremely large or very small. For example- I may have 6,500,000,000 red blood cells, or the diameter of an iron atom is 0.000000011 inches. Numbers with many zeros can be inexpedient to work with, so scientists use scientific notation. It represents very large numbers or very small numbers in a simple form. We know that whole numbers can be extended to infinity, but we cannot write such large numbers on paper. Numbers that present in millions of places after the decimal point need to be represented simply. Thus, it is difficult to represent a few numbers in their extended form. Therefore, we use scientific notation.