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State the functions for each of these components used in the gel filtration chromatography using a column experiment.
- Bromophenol blue
- A mixture of albumin and bromophenol blue
- Spectrophotometer
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- Label the void volume (V0), elution volume (Ve) and total elution volume (Vt) appropriately on the graph for the gel filtration chromatography using a column experiment.how to calculate the experiment fow rate for this gel chromatography Experiment flow rate ? Flow rate 4.75mL/minbriefly explain the principle behind this technique; gel-filtration chromatography
- Briefly explain the principle behind the following techniques:(a) ion-exchange chromatography (b) gel filtration chromatography (c) affinity chromatographyHow would you make two-fold serial dilutions such that the last tube is a 1:32 dilution of the original, concentrated material? Assume that you need to have at least 500 µl of each dilution, and you should be able to perform the dilutions in microfuge tubes with a maximum capacity of 1.5 ml.The following image is a scheme for serial dilutions prepared for spectrophotometric analysis. If the stock solution concentration is 0.05 % (v/v) can you calculate the other tube’s concentrations in % v/v? I've used this with direct dilutions, how would I use this on serial dilutions?
- With the aid of diagrams, explain the processes and principles of each of the following methods: (a) Gel filtration chromatography on Sephadex G50 b.SDS-PAGEWhat are the mechanisms of samples separation work in Thin layer Chromatography? Please shortly write at your own words. Answer should be to the point (5-6 lines maximum).By applying Gravimetry, Titration (Chemical indicator) and Potentiometry lessons which technique should be used in following situations. Explain your answer. In your explanation, state what is the advantage of the technique you chose over other technique and /or the barrier/difficulty of using the technique. Your choice should not be dependent on cost. For chem students- Total Acid content per tablet of a newly developed drug that has yellow coating Target level is 1%.
- With the constants you have for each objective, compute for the sizes of the given cells below. (Refer to the illustrations & show your solutions). Also, name each specimen used.Answer the ff and state references: For his experiment, Brian needs to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big test tube) of Luria What is the total volume of the medium needed? What is the amount of each component needed, given the following media composition per liter of distilled water: Component g/L tryptone 10.0 NaCl 5.0 yeast extract 10.0 agar 15.0Answer the following questionsExplain in your own words how the ISO 10993-5 test (performance test) is applied to the patient turning apparatus shown in the figure, and guess how the expected result will be. The main part of the product consists of a polyurethane sponge. Stain-proof upholstery fabric will be used as the outer surface material. In addition, there are heating pads working with the logic of electric blankets in this apparatus, please answer by taking these into account when answering.