Calculating Transformation Efficiency

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
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Calculating Transformation Efficiency
Transformation Efficiency is defined as the number of colony forming units (cfu) which
would be produced by transforming 1 ug of plasmid DNA into a given volume of competent
cells.
Transformation Efficiency (TE) = number of cfu/ug DNA
You have just transformed 1 ul (100 pg/ul) of control pWasabi (plasmid) DNA into 50 µl of
E. coli DH5a by the heat-shock method. You then add 950 µl of SOC medium to outgrow the
bacteria. Of this, you plated 50 µl onto a LB plate containing ampicillin. After overnight
incubation, you observed that there are 150 colony forming units (cfu) on the plate. Calculate
the transformation efficiency (show your step-wise calculations below).
Transcribed Image Text:Calculating Transformation Efficiency Transformation Efficiency is defined as the number of colony forming units (cfu) which would be produced by transforming 1 ug of plasmid DNA into a given volume of competent cells. Transformation Efficiency (TE) = number of cfu/ug DNA You have just transformed 1 ul (100 pg/ul) of control pWasabi (plasmid) DNA into 50 µl of E. coli DH5a by the heat-shock method. You then add 950 µl of SOC medium to outgrow the bacteria. Of this, you plated 50 µl onto a LB plate containing ampicillin. After overnight incubation, you observed that there are 150 colony forming units (cfu) on the plate. Calculate the transformation efficiency (show your step-wise calculations below).
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