why chromotography isn't a good fit to detemine mitochondrial RNA polymerase
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why chromotography isn't a good fit to detemine mitochondrial RNA polymerase
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- Question. Rewrite the following sentences after correction. (Subject: Biotechnology) The variation in the length of tandem repeat of microsatellite DNA has serious translational affects as this is due to its coding region. Correct: If one parent has sickle cell anemia and other has carrier genotype than there is 25 % chance that any offspring is carrier. Correct: Sickled WBC block the flow of blood and Calcium as they stick together and caused by frame shift mutation. Correct: The N1303K mutation in the CFTR gene of CF patients is autosomal dominant disorder due to insertion of asparagine at 1303. Correct: If a person RBCs have B surface antigen and it will clump with antigen B such clumping indicates Blood type B. Correct: Indirect ELISA can detect polygenic gene expression. Correct:Question:- All the necessary ingredients for the Polymerase Chain Reaction (PCR) are mixed together in a PCR tube and placed in the thermal cycler. The DNA polymerase is from Thermus aquaticus,the template is human DNA, and the primer is complementary to the gene for a human protein. What would result from amplification? Group of answer choices a.a mixture of human DNA, RNA, and protein b.human DNA c.human protein d.T. aquaticus DNA e.a mixture of human and T. aquaticus DNATopic: Recombinant pharmaceuticals (for the production of insulin, human growth hormone or blood clotting factors) Question When or why is this genetic technology/process used? Who benefits from this genetic technology/process - and how?
- Need to understand how to do this: create the complementary strand of DNA to the DNA strand provided below: 1. ATAGGTCACCGTAA 2. TCCAGAquestion- If you wanted to make a cDNA library , which of the following enzymes would you definitely NOT need? SELECT ALL THAT APPLY. RNA polymerase, Cas9, Restriction enzymes, DNA ligase, Reverse transcriptase, PhosphataseQUESTION: Explain the processes of the following experiments: Gel Electrophoresis, Plasmid Isolation, and Plasmid Injection into C. Elegans nematode. and why we are doing these experiments, what is the major outcome, and what can be achieved by applying these techniques in medicine?
- need help. For both question 20. The proofreading ability of DNA polymerase which depends on its native exonuclease activity occurs in the: A. 5' to 3' direction B. 3 to 5' direction 21. Direct repair mechanisms in plants involve the enzyme: A Photolvase B. Convertese C. Hydrolase D. PhosphodiesteraseConclusions and Future utility about DNA Barcoding and Woolly mammoth diet mystery solved by DNA analysis? 3 Discussion Questions about DNA Barcodinggive answer for both questions and detail answer needed ... asap ..dont copy from any other site ..there is already a answer in chegg so
- Transcribed Image Text:Complete the following tasks. You discovered that a species of bacteria can break down StyrofoamT (polystyrene) products due to an enzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme. Task 1: DNA Extraction To begin work on the bacterium, you begin by extracting its genomic DNA (GDNA). What is the purpose of the following procedures? Answer briefly but completely. Using sodium dodecyl sulfate, a detergent Answer: а. b. Adding RNase A and Proteinase K during extraction Answer: c. Adding ethanol before recovering the DNA extract С. Answer: Task 2: Polymerase Chain Reaction After purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCR using the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answer briefly but completely. DNA polymerase isolated from Thermus aquaticus Answer: а. b. Deoxynucleotide triphosphates (DNTPS) Answer: С. Forward and…Question:- In an early log-phase E. coli population, there are 1 x 103 cells/ml the generation time of the cells is 30 minutes. How many cells would there be 60 minutes later? a. 2X10^6b. 8X10^3c. 4X10^3d. 2X10^3e. 3X10^6QUESTION- There is an RFLP pattern that belongs to a disease. I need to find an inheritance pattern and marker related to the disease..