can you complete table 4.7, using Beer's law. where C= .04 micromoles, e= 18.8850, and b= 1 cm

Chemistry: Principles and Reactions
8th Edition
ISBN:9781305079373
Author:William L. Masterton, Cecile N. Hurley
Publisher:William L. Masterton, Cecile N. Hurley
Chapter1: Matter And Measurements
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can you complete table 4.7, using Beer's law. where
C= .04 micromoles, e= 18.8850, and b= 1 cm
TABLE 4.6
ABSORBANCE
WAVELENGTH
ABSORBANCE
WAVELENGTH
0,6488
O.61053
0.54797
0.40397
0.41838
385
390
415
410
405
400
395
0.65167
0.708
0.74448
0.1S4 52
0,74318
0.71471
375
370
365
390
A 400
A-e bc
3.
Calculation of concentration of p-nitrophenol added (Notenstock concentration 0.2mM):
C2MM)(Iml)=C(5mi) 0.04:4 C,= Iml
Calculation of t, the molar extinction coefficient:
try
CI)C 0004 8850
TABLE 4.7
TYPE OF
BUFFER
ABSORBANCE
pH
(A 1
(HA]
Tris-HCl
Tris-Hl
phusphate
phispmte
phusphate
Lonsphale
9.0
8.0 0702
7.5 0.556
7.0 0.385
6.5 0.192
6.0
RY
Transcribed Image Text:can you complete table 4.7, using Beer's law. where C= .04 micromoles, e= 18.8850, and b= 1 cm TABLE 4.6 ABSORBANCE WAVELENGTH ABSORBANCE WAVELENGTH 0,6488 O.61053 0.54797 0.40397 0.41838 385 390 415 410 405 400 395 0.65167 0.708 0.74448 0.1S4 52 0,74318 0.71471 375 370 365 390 A 400 A-e bc 3. Calculation of concentration of p-nitrophenol added (Notenstock concentration 0.2mM): C2MM)(Iml)=C(5mi) 0.04:4 C,= Iml Calculation of t, the molar extinction coefficient: try CI)C 0004 8850 TABLE 4.7 TYPE OF BUFFER ABSORBANCE pH (A 1 (HA] Tris-HCl Tris-Hl phusphate phispmte phusphate Lonsphale 9.0 8.0 0702 7.5 0.556 7.0 0.385 6.5 0.192 6.0 RY
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