Choose the best combination of depyrogenation methods: 0.45 um filtration +0.22 µm filtration OUV+0.45 um filtration 0.22 um filtration + UV treatment 0.45 um filtration + distillation 0.22 um filtration + distillation
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- DILUTION COLONY COUNT CFU/mL 1:106 155000 1:107 15500 1:108 1550 1:109 155 Given these values how would I fill in the rest of this serial dilution table? Also, what would be a 1:1 CFU/mL value based on this table?During reflux, what is the most suitable heater setting? How long the liquid needs to be boiled at this heat setting? options: A) Heater setting = 9, Continue heating till 2-3 mL of the liquid is left behind in the boiling flask. B) Heat setting = 9 for 30-45 minutes C) Heat setting = 6 for 30-45 minutes D) Heat setting = 5 for 45-60 minutesA sample is diluted by a factor of 10 five times. The 10^-3 dilution has 272 colonies on it. Assuming you plated the same volume on each plate, how many colonies would you expect to find on the 10^-2 plate? Include units and use OCD formula.
- From a 5% normal saline (NaCl) solution, take 2 ml and dilute it 1:5. Take 5 ml of this solution and dilute it 1:10. A 2:100 dilution is made from this last solution. Determine: a) How many times less concentrated is the 2nd dilution than the original? b) What is the dilution factor in each step of the series and what is the final volume of each dilution? c) How many grams of NaCl will be in 1 ml of the last solution?I do not understand the results of this experiment, explain what the results of this experiment are and the purpose Table 2 Absorbance vs CoCl2 concentration Test Tube Number Cobalt Chloride Concentration (mol/mL) Absorbance at 510 nm 1 0.000 0.000 2 0.009 0.024 3 0.018 0.055 4 0.027 0.085 5 6 0.036 0.045 0.112 0.138 7 (Unknown) 0.088Order: streptomycin 500 mg IM daily for 8 days. If you have vials of this antibiotic that are labeled as 1 g/2.5 mL, how many milliliters would you administer?
- You perform a series of 4 different dilutions of 200 µL of sample into 800 µL of saline. What is the final dilution factor versus the initial sample?A 99Mo/99mTc generator is elute Monday at 7 am, producing 1.8Ci of 99mTc inthe eluate vial, in 20ml saline. What volume of eluate should be withdrawn fromthe eluate vial into a syringe to inject a patient with 20mCi of Tc-99m at3pm?Gel-filtration chromatography separates molecules according to their size . Smaller molecules diffuse faster in solution than larger ones, yet smaller molecules migrate more slowly through a gel- filtration column than larger ones. explain this paradox. What should happen at very rapid flow rates?
- Label the void volume (V0), elution volume (Ve) and total elution volume (Vt) appropriately on the graph for the gel filtration chromatography using a column experiment.Your patient is receiving 1000ml NS by IV at a rate of 75ml/hr. How much NS will he get in 5 hours?Refer to the chromatograph (Figure 1) below and answer the questions that follow: What is the volumetric flow rate of the column? What type of compound is likely to be found in peak B? Explain. What possible buffer changes could the researcher have applied at the 30ml mark? Explain. How would one avoid cross-contamination when using this type of purification