Refer to the chromatograph (Figure 1) below and answer the questions that follow: What is the volumetric flow rate of the column? What type of compound is likely to be found in peak B? Explain. What possible buffer changes could the researcher have applied at the 30ml mark? Explain. How would one avoid cross-contamination when using this type of purification
Q: Calculate the resulting equilibrium concentrations of all four species. Express your answers to four…
A: In a general reaction such as: aA + bB ⇌ cC + dD At equilibrium, the concentration of reactants and…
Q: Given the following reaction below, what amino acid is involved and what is the specific reaction…
A: Phenylalanine, tyrosine, and tryptophan are aromatic amino acids. The side chain of the…
Q: ● What are the three things we need to know in order to begin to understand the way an active site…
A: Enzymes are high molecular weight proteins that catalyse biochemical reactions. The substrate binds…
Q: Draw a table that shows the monomer component and an example for each macromolecule
A: The four classes of biological macromolecules are nucleic acids, proteins, carbohydrates and lipids.…
Q: The proton-motive force is a measure of the potential energy generated across the mitochondrial…
A: INTRODUCTION : Proton-motive force : It is a measure of the potential energy generated across the…
Q: Chemical biologists view metabolism's citric acid cycle as its fulcrum. The enzyme isocitrate…
A: Citric acid cycle: (Krebs cycle) Citric acid, a tricarboxylic acid that is frequently referred to…
Q: 4. Below each item, identify WHAT it is, indicate WHERE in the cell it is used/made (cytoplasm or…
A: Glycolysis is the metabolic pathway that converts glucose into pyruvate. The free energy that is…
Q: Calculate the net charge on the following tripeptides at pH 5.0: (a) Leu-His-Asp [0] (b)…
A: Recall that: Amino acid sequences are written with N-terminal amino acid on the left and C-terminal…
Q: A D. J B с
A: Reactions can proceed forward or reverse reactions can proceed spontaneously without the help of…
Q: 8. Anaplerotic and amphibolic reactions in TCA.
A: In aerobic condition, pyruvate in the presence of pyruvate dehydrogenase complex produces Acetyl…
Q: 1. What two enzymes in glycolysis are regulated by high ATP concentrations?
A: INTRODUCTION : Glycolysis : It is a metabolic pathway in which glucose is breaken down into pyruvate…
Q: Why do some starch products set as they cool?
A: Introduction Starch is a carbohydrate which is commonly found in nature like wheat, rice, potatoes…
Q: For the following scenarios, determine whether the molecules in the scenario are moving by simple…
A: Biological membranes are structures that surround the cell or organelles and act as barriers. An…
Q: Briefly describe the role of glycoproteins as antigenic determinants for blood groups.
A: Glycoproteins are proteins with carbohydrates attached to it. The carbohydrates that are commonly…
Q: In the free energy diagram below, molecule A (with energy x) is converted to molecule B (with energy…
A: Activation energy for a reaction is the minimum amount of extra energy that is necessary for the…
Q: is lapoamide involved in oxidation reduction..
A: INTRODUCTION : First of all there is no such proper biochemical name like Lapoamide(its incorrect) I…
Q: Among the given statements, which are characteristics of plant cells? Select the correct…
A: Plant cell has Cell wall as the outermost layer. Cell wall being the outermost layer surrounds the…
Q: 1.0 E oxygen saturation (Y) 0.6 0.4 0.2 0.0 20 40 60 blood pO₂ (torr) 80 100 120 for this picture…
A: Hemoglobin carries oxygen from the lungs to the tissues and CO2 from the tissues to the lungs. When…
Q: What charged groups are present in leucine at a pH = 7? OA) 1× NH3¹ B) 1 x COO OC) 1× NH3¹ and 1 ×…
A: Amino acids have a central carbon atom known as α-carbon atom. Each amino acid has an amino group,…
Q: Calculate the pI for the following peptide: Phe-Lys-Glu-Asp-Lys-Ser-Ala (note: there is only one…
A: Amino acid sequences are written with N-terminal amino acid on the left and C-terminal amino acid on…
Q: Suppose there is a glycolytic-like pathway carried out by an alien species that uses analogous…
A: The reversible aldolase reaction in glycolytic pathway cleaves a 6 carbon keto-bisphosphate into a 3…
Q: 4. A single base addition and a single base deletion approximately 15 bases apart in the mRNA…
A: As per the central dogma of molecular biology, the genetic information stored in the DNA is copied…
Q: Please write down the DNA sequence inferred from the below DNA gel. Shown are the products of a…
A: DNA is the genetic material in most living organisms. The information in a DNA molecule is encoded…
Q: 27. While culturing some cells, you lower the temperature of the culture. What happens immediately…
A: Cell membrane are composed of lipid bilayer and proteins. Membrane fluidity depends on the content…
Q: 1. Calculate the size of the resulting fragments as they will occur after digestion and write the…
A: Lambda DNA is a linear DNA extracted from E coli bacteriophage with 485052 base pairs (bp) At each…
Q: Identify the type of chemical reaction. 1 R R 2 Fat / Oil C01695 -R 3 NaOH/H₂O heat Lye Linoleic…
A: Triglycerides are fatty acid esters of glycerols. Fatty acids are carboxylic acids with a long…
Q: Question 7 In the human body, under oxygen rich and oxygen poor conditions, respectively, pyruvate…
A: Respiration at molecular level refers to the process through which cells catabolize biomolecules…
Q: Insulin deficiency (occurrence/factors) factors affecting insulin levels including biological,…
A: The hormone insulin is in charge of permitting blood glucose to enter cells, giving them the energy…
Q: Question 11 of 25 Among the given statements, which are characteristics of plant cells? Select the…
A: Osmosis is the process of net movement of water across a semipermeable membrane This transport…
Q: What compound of phosphorus is found in nucleic acids? What are the products of hydrolysis in RNA…
A: DISCLAIMER FOR MULTIPLE Since you have asked multiple question, we will solve the first question…
Q: In a healthy young adult, what may explain high levels of creatinine in serum and urine samples?
A: INTRODUCTION : Creatinine - It is a breakdown or by- product of creatine phosphate from muscles and…
Q: Exergonic and endergonic biochemical reactions; the role of ATP and other macroergic substances in…
A: INTRODUCTION : ATP - Adenosine triphosphate, it is the main energy currency or units, which is…
Q: Draw his trp arg pro glu met ile | PH = 2 1b) How many possible structures are there for this? 1c)…
A: Peptides are composed of amino acids. Amino acids are biomolecules with an alpha carbon bonded to an…
Q: Analyze each item carefully and write your complete solution. Cysteine is an important amino acid…
A: Note: As per the honor code we are allowed to answer the first two questions. Kindly resubmit the…
Q: The function of the Electron Transport Chain (ETC) in eukaryotic cells is to produce a proton…
A: In the electron transport chain (ETC), the electrons pass through four enzyme complexes, that…
Q: Can you please Draw what this should look like. Chromosome Model Procedure for making a solid wire…
A: Chromosomes The histone proteins H1, H2A, H2B, H3, and H4 assist the DNA to be compressed and…
Q: The figure below illustrates what reactions of amino acids? coo &C=0 CH₂ CH₂ 0=( 00 + NHⓇ NAD(P)H+H…
A: The amino group, carboxyl groups, and side chains of amino acids all play a role in how amino acids…
Q: Define the planted motif search problem.
A: INTRODUCTION : A sequence motif - This sequence motif is a nucleotide or amino acid sequence pattern…
Q: What is the role of ribose in Central Dogma of Molecular Biology?
A: The information stored in the DNA flows to RNA and is then used in the synthesis of proteins. This…
Q: Nucleic Acid Chromatogram O O Nucleic acid chromatogram with nucleic acids circled in pencil 00
A: Chromatography is a biochemical technique that is used to separate closely related substances from a…
Q: Can I please get help? 1. Describe the morphological phenotypes you see in the experimental…
A: Neurons: Sending, receiving, and transmitting electrochemical impulses throughout the body is the…
Q: The activity of an enzyme can be regulated by a: A) competitive inhibitor binding to the active…
A: The enzymes increase the rate of biochemical reactions and can be regulated by binding to…
Q: Select the chemical consequences that could contribute to DNA instability at AP sites. fewer…
A: AP sites are apuraniya sites that are created through the hydrolysis of N glycosyl bond between…
Q: Calculate 1/[S] and 1/V to complete the table. Use this data to draw a Lineweaver-Burke plot, with…
A: A Lineweaver-Burk plot represents the relationship between the reciprocal values of substrate…
Q: In the liver, what is the major regulation that dictates fate of pyruvate vs lactate? (This can tie…
A: Pyruvate that is produced as the end product of glycolytic pathway is converted into acetyl CoA…
Q: Biochemical events in the synthesis of ATP: I. Subunits of ATP synthase chages in conformation and…
A: ATP synthase is a complex enzyme consists of an F0 and F1 components. It uses the proton motive…
Q: DNA: What happens in each stage or phase of transcription (initiation, elongation, termination)?
A: Transcription is the process of Synthesis of mRNA from DNA by RNA polymerase. It uses 3'-5' strand…
Q: What is ATP? in 5 sentences Why is ATP important in the body? in 5-7 sentences
A: There are many forms of energy. But living system needs one form of energy i.e., chemical energy.…
Q: A 100 mL solution of 0.1 M amino acid (AA) at pH 1.0 was titrated with 1.0 M NaOH solution. The pH…
A: Amino Acids: All amino acids have an amine and a carboxylic acid functional group with acid/base…
Q: Which of the following is correctly classified? O Arachidic (20:0) is a medium chain unsaturated…
A: Fatty acids are carboxylic acids with a hydrocarbon chain ranging from 4 carbon to 36 carbons. The…
Refer to the chromatograph (Figure 1) below and answer the questions that follow:
- What is the volumetric flow rate of the column?
- What type of compound is likely to be found in peak B? Explain.
- What possible buffer changes could the researcher have applied at the 30ml mark? Explain.
- How would one avoid cross-contamination when using this type of purification
Step by step
Solved in 2 steps
- Infuse heparin at 1,200 units per hour from a solution containing 40,000 units of heparin in 500 mL D5W. How many mL/hr will deliver the ordered dosage?The octapeptide gly-cys-met-asn-lys-ala-tyr-gly was hydrolyzed consecutively by CNBr and then trypsin (3 fragments total). The mixture of fragments was buffered at a pH 8.5 and then chromatographed on an anion-exchanger (positive resin) with the same buffer. Draw sequence (abbreviated form) of all fragments at that pH (show charges) and predict elution order.A vial of Doxorubicin reads 0•5g per vial. Instructions say to reconstitute each 12mg with 2•5ml of NS. How many ml of NS will be needed to reconstitute the vial of the recommended concentration? please show working
- Please help me answer and explain these questions: 1. Compare the abundance of A280 chromophores (W, Y, and C) to Bradford sites (loosely R and K) in BSA. 2. We used a protocol that corrects the path length in each well and normalizes the absorbance to the value that would be obtained if the path length were 1 cm (the standard path length). Using this observation and your A280 calibration data, what is your measured e280, for BSA?80mL of a 0.3M solution of hexapeptide Leu-His-Cys-Glu-Asn-Arg is adjusted to pH=pI. The solution is then titrated with 0.2M HCL to a final pH of 2.1. Sketch the titration curve, labelling the pH and volume axes. Indicate the volume of HCL needed to reach relevant pKa value and equivalence point(s)z Relevant pKa values are: 2.1, 4.3, 6.0, 8.3, 9.8, and 12.5.Hi! For the first part, you need to put appropriate reagent (found below the diagram) to the space provided that will yield the given results. For the second part, you need to determine the reagent/s needed to confirm the identity of the cation from question 1. There can be more than one answer. Thank you!
- The diffusivity of amino acids in polyacrylamide gel is approximately 1x10^-9 cm2./s calculate the initial flux of amino acids, give an instantaneous gradient of (20g/cm 3 )/8cm Why is polyacrylamide gel is used in electrophoresis?Help please. MD ordered morphine SO4 gr 2/5 and the pharmacist has a stock solution of gr 1/8 per milliliter of morphine SO4. How many milliliters of the stock solution is required to fill the Rx?You receive an order for “heparin 6,000 units SQ q 12 hrs”. The stock solution contains heparin 5,000 units per 0,5 mL. How many milliliters must be administered?
- (a) The octapeptide AVGWRVKS was digested with the enzyme trypsin . Which method would be most appropriate for separating the products: ion-exchange or gelfiltration chromatography? Explain. (b) Suppose that the peptide was digested with chymotrypsin . What would be the optimal separation technique? Explain.You perform a Bradford assay to determine the concentration of isolated α-lactalbumin. You use 50 μL of a two-fold diluted solution of α-lactalbumin in the assay. You generate a standard curve with the following equation for the line: y = 0.163x + 0.082. The absorbance of your sample was 0.674 AU. What is the concentration of α-lactalbumin, in mg/mL, in your sample? Give your answer to three significant figures.Chitinase is a protein that breaks down chitin, a primary component of the cell wall in fungi, scales in fish and exoskeletons of arthropods. The activity of chitinase extracted from a plant was shown to be optimum at pH 5. You were tasked to prepare 300 mL of 150 mM buffer solution for further analysis of the extracted chitinase. REAGENTS Ka 2.5M Acetic acid Solid NaOAc•3H2O [136.08g/mol] 1.76 x 10-5 2.5M NH3 Solid NH4Cl [53.49g/mol] 5.6 x 10-10 2.5M Lactic acid Solid sodium lactate [112.06g/mol] 4.0 x 10-5 5 M HCl 5M NaOH Pls show sol'ns 1. Given the following reagents, give the moles of each component (acid & base).2. What are the mass/volume of the components needed to prepare the buffer? 3. What will the pH of the buffer be if 1mL of 5 M NaOH was added?