Refer to the chromatograph (Figure 1) below and answer the questions that follow: What is the volumetric flow rate of the column?  What type of compound is likely to be found in peak B? Explain.  What possible buffer changes could the researcher have applied at the 30ml mark? Explain.  How would one avoid cross-contamination when using this type of purification

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter6: Oral Medication Labels And Dosage Calculation
Section: Chapter Questions
Problem 7.2P
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Refer to the chromatograph (Figure 1) below and answer the questions that follow:

  1. What is the volumetric flow rate of the column? 
  2. What type of compound is likely to be found in peak B? Explain. 
  3. What possible buffer changes could the researcher have applied at the 30ml mark? Explain. 
  4. How would one avoid cross-contamination when using this type of purification 
A280
- па
10
20
30
Volume (ml)
B
40
50
Figure 1: Affinity chromatography of semi-purified extract on a lectin-sepharose column. Column
dimensions are 2cm i.d. x 5cm, flow rate is 10cm/hr.|
Transcribed Image Text:A280 - па 10 20 30 Volume (ml) B 40 50 Figure 1: Affinity chromatography of semi-purified extract on a lectin-sepharose column. Column dimensions are 2cm i.d. x 5cm, flow rate is 10cm/hr.|
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