Colonies/ug of plasmid) based on these results? O about 140O O about 800 O about 4000 O about 400 about 8000

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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You have just carried out a transformation using a plasmid (possessing a
Amp-resistance gene) that was 0.001 ug/ul in concentration. You added
10 ul of this plasmid to 100 ul of a bacterial cell suspension. Then
you
added 250 ul of LB following heat shock. After plating 200 ul of this cell
suspension with LB onto a LBA plate, you observe 8 colonies on this
plate. What is the transformation efficiency of this plasmid (in
colonies/ug of plasmid) based on these results?
about 1400
O about 80O
about 4000
O about 400O
O about 8000
f6
米
IO
fs
fg
f1o
19
08.
O OO
Transcribed Image Text:You have just carried out a transformation using a plasmid (possessing a Amp-resistance gene) that was 0.001 ug/ul in concentration. You added 10 ul of this plasmid to 100 ul of a bacterial cell suspension. Then you added 250 ul of LB following heat shock. After plating 200 ul of this cell suspension with LB onto a LBA plate, you observe 8 colonies on this plate. What is the transformation efficiency of this plasmid (in colonies/ug of plasmid) based on these results? about 1400 O about 80O about 4000 O about 400O O about 8000 f6 米 IO fs fg f1o 19 08. O OO
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