4. This is the set-up of our experiment. There are four platesshown: LB only (a type of nutrient agar), LB/AMP (LBagar with ampicillin included), and LB/AMP/Arabinose(LB agar with ampicillin and arabinose). All plates werespread with E. coli following the transformation proceduredescribed in the video. Some of the E. coli samples weremixed with pGLO plasmid DNA (+DNA samples) andsome E. coli samples were not (-DNA).Plate B-DNAPlate ALBLB/AMPPlate CPlate D+DNALB/AMPLB/AMP/ARA5. Complete the following chart to walk through the resultsyou expect to see from the transformation procedure.2Based on B, IfE. colicoli added to coli ampicillin should the E. should grow, included as controlscoli havegrown on the glow?Did the E.Was the E.Plates A & B arethe plate have resistant?the PGLOplasmid?(How do you plate?figure thisout?)should it also for the lab. What dothese plates tell you?(If no growth onplate A or growth onplate B)Plate ALB (generalgrowth media)Plate BLB + amp(ampicillin)Plate CLB + ampPlate DLB + ampara (arabinose)00

Bacterial transformation is the process by which bacterial competent cells take up naked DNA. The…

This is the set-up of our experiment. There are four plates shown: LB only (a type of nutrient agar), LB/AMP (LB agar with ampicillin included), and LB/AMP/Arabinose (LB agar with ampicillin and arabinose). All plates were spread with E. coli following the transformation procedure described in the video. Some of the E. coli samples were mixed with PGLO plasmid DNA (+DNA samples) and some E. coli samples were not (-DNA).

This is the set-up of our experiment. There are four plates shown: LB only (a type of nutrient agar), LB/AMP (LB agar with ampicillin included), and LB/AMP/Arabinose (LB agar with ampicillin and arabinose). All plates were spread with E. coli following the transformation procedure described in the video. Some of the E. coli samples were mixed with PGLO plasmid DNA (+DNA samples) and some E. coli samples were not (-DNA).

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 4TYK

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We want to extract DNA from this culture of bacteria suspended in Media the bacteria cells are floating in the liquid. Our first step is to centrifuge our sample. Do we keep the supernatant or the pellet and why? A. Supernatant because it contains Calais or nutrients B. Pellet because it contained only DNS and the supernatant contains the rest of the cell part C. Supernatant because it contains only dna and the pellet contains the rest of the cell parts D. Pellet because it contains the parts of the bacterial cell with the plasmid DNA
Researchers are designing several experiments to test the ability of Salmonella bacteria to develop antibiotic resistance. A culture of Salmonella bacteria is exposed to the same concentrations (200 mg/L) of an antibiotic for four days. The table shows the number of isolated resistant bacteria over a four-day period. Which of the following statements best explains these results? A - The bacteria were not affected by the antibiotic. B - After being exposed to the antibiotic, the bacteria altered their DNA. C - A new species of bacteria emerged after the antibiotics were introduced. D - Random mutations led some bacteria to be resistant and, over time, they increased in the population.
Could you explain how you would know if you successfully induced transformation? Thank you Did you successfully demonstrate transformation occurred? Why or why not? (How do I know, what do I look for?) Explain the control used in this experiment. What was it, and why was it necessary? Based on the calculation in the question above, comment on the relative difficulty of achieving successful transformation of E. coli. (Please explain the relative difficulty)
1. What is the purpose of adding of (a) warm salt water, (b) detergent, and (c) alcohol on the experiment of the extraction of DNA from a banana?
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You discovered that a species of bacteria can break down StyrofoamTM (polystyrene) products due to anenzyme it produces, polystyrenase. You wish to study the gene that codes for this enzyme.Task 1: DNA ExtractionTo begin work on the bacterium, you begin by extracting its genomic DNA (gDNA). What is the purpose ofthe following procedures? Answer briefly but completely.a. Using sodium dodecyl sulfate, a detergentAnswer: __________________________________ b. Adding RNase A and Proteinase K during extractionAnswer: __________________________________ c. Adding ethanol before recovering the DNA extractAnswer: __________________________________ Task 2: Polymerase Chain ReactionAfter purifying the gDNA extract, you want to isolate and amplify the polystyrenase gene. You perform PCRusing the appropriate gene-targeted primers. What is the purpose of the following PCR components? Answerbriefly but completely.a. DNA polymerase isolated from Thermus aquaticusAnswer:…
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1. You have an F+ his- leu+ strain of E. Coli as well as a F- his+ leu- strain. Describe a strategy for how you might use these to isolate an Hfr strain.
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