Compare Vmax and Km of the enzyme without inhibitor and in the presence of acetazolamide. Determine the type of inhibition shown by acetazolamide. Explain your answer. (i) (ii) Name TWO (2) other types of inhibitions besides the inhibition shown by acetazolamide in Qla)(i). List down the kinetic properties of these inhibitions. Sketch a graph of I/V versus 1/[S] showing plots in the absence of an inhibitor and in the presence of the types of inhibitors mentioned in Qla)(ii). (ii)
Compare Vmax and Km of the enzyme without inhibitor and in the presence of acetazolamide. Determine the type of inhibition shown by acetazolamide. Explain your answer. (i) (ii) Name TWO (2) other types of inhibitions besides the inhibition shown by acetazolamide in Qla)(i). List down the kinetic properties of these inhibitions. Sketch a graph of I/V versus 1/[S] showing plots in the absence of an inhibitor and in the presence of the types of inhibitors mentioned in Qla)(ii). (ii)
Chapter10: Reconstitution Of Powdered Drugs
Section: Chapter Questions
Problem 25SST
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Question
![Figure I shows the Michaelis Menten plot of initial reaction velocity (as percentage of Vmax)
versus [S] (concentration) for the carbonic anhydrase reaction in the absence and presence of
the inhibitor acetazolamide. Carbonic anhydrase participates in regulation of the pH and
bicarbonate content of a number of body fluids.
100
No inhibitor
Acetazolamide
0.2
0.4
0.6
0.8
1
[S] (mM)
Figure 1
(i)
Compare Vmax and Km of the enzyme without inhibitor and in the presence of
acetazolamide. Determine the type of inhibition shown by acetazolamide. Explain
your answer.
(ii)
Name TWO (2) other types of inhibitions besides the inhibition shown by
acetazolamide in Qla)(i). List down the kinetic properties of these inhibitions.
Sketch a graph of I/V versus 1/[S] showing plots in the absence of an inhibitor and in
the presence of the types of inhibitors mentioned in Qla)(ii).
(iii)
V (% of Vmax](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fa56c35a6-4623-4311-a953-bfaba84ab3d3%2F3aca18f6-9343-4b4f-90e1-8d4103cafa5a%2Feq02goo_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Figure I shows the Michaelis Menten plot of initial reaction velocity (as percentage of Vmax)
versus [S] (concentration) for the carbonic anhydrase reaction in the absence and presence of
the inhibitor acetazolamide. Carbonic anhydrase participates in regulation of the pH and
bicarbonate content of a number of body fluids.
100
No inhibitor
Acetazolamide
0.2
0.4
0.6
0.8
1
[S] (mM)
Figure 1
(i)
Compare Vmax and Km of the enzyme without inhibitor and in the presence of
acetazolamide. Determine the type of inhibition shown by acetazolamide. Explain
your answer.
(ii)
Name TWO (2) other types of inhibitions besides the inhibition shown by
acetazolamide in Qla)(i). List down the kinetic properties of these inhibitions.
Sketch a graph of I/V versus 1/[S] showing plots in the absence of an inhibitor and in
the presence of the types of inhibitors mentioned in Qla)(ii).
(iii)
V (% of Vmax
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