COMPLETE ALL CALCULATIONS REQUIRED FOR TABLE 1 ( For all measurements, the [PNPP] concentration will be 600 μM in a 1 ml final volume.) PNPP (substrate) stock solution: 100 mM PNPP in buffer 2. IN YOUR LAB NOTEBOOK, DRAW YOUR PREDICTION OF WHAT THE FOLLOWING GRAPHS MIGHT LOOK LIKE a. pH vs. V0 b. TEMP vs. V0

Curren'S Math For Meds: Dosages & Sol
11th Edition
ISBN:9781305143531
Author:CURREN
Publisher:CURREN
Chapter6: Oral Medication Labels And Dosage Calculation
Section: Chapter Questions
Problem 9.1P
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PRE-LAB ASSIGNMENT – (YOU WILL NOT BE ALLOWED TO BEGIN WORK ON LAB
WITHOUT THE COMPLETED CALCULATIONS)


1. COMPLETE ALL CALCULATIONS REQUIRED FOR TABLE 1 ( For all measurements, the [PNPP] concentration will be 600 μM in a 1 ml final volume.) PNPP (substrate) stock solution: 100 mM PNPP in buffer

2. IN YOUR LAB NOTEBOOK, DRAW YOUR PREDICTION OF WHAT THE
FOLLOWING GRAPHS MIGHT LOOK LIKE
a. pH vs. V0
b. TEMP vs. V0
3. OUTLINE THE BEST/MOST EFFICIENT WAY TO PREPARE

Table 1
Total volume (ul) PNPP stock (ul)
1000
Buffer (μl)
Enzyme (ul)
5
Final [PNPP] (uM)
600
Transcribed Image Text:Table 1 Total volume (ul) PNPP stock (ul) 1000 Buffer (μl) Enzyme (ul) 5 Final [PNPP] (uM) 600
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