Q: In the isolation of DNA, what is the role of chloroform? choose the best asnswer -Precipitate rna…
A: DNA extraction is the process of isolating DNA from the cells of an organism isolated from a sample,…
Q: (c) Based on the primary sequence surrounding the transmembrane portion, predict which Portion would…
A: The chemical structure of the 5-HT 2A receptor and its interactions with psilocybin and psilocin are…
Q: What material would normally be found moving through the lumen of the small intestine?
A: Introduction : Small intestine is highly coiled organ of the digestive system. It is having three…
Q: What differences exist within keratinocytes of the superbasal epidermis between individuals of dark…
A: Introducion Skin is the most important organ of human body. Skin acts as a barrier in human body.…
Q: segment shown in the diagram. The first three nucleotides of sequencing read 1 and sequencing read…
A: Nucleotides are the building blocks of DNA and RNA. They are organic molecules that consist of a…
Q: Large international variations in rates of communicable diseases, undernutrition, and maternal and…
A: Introduction : Pathogens are microscopic organisms that cause diseases in humans and kther…
Q: Activation of 5-HT 24 Increases cytosolic free Ca²+ Concentration ([Ca²+ ]) Via a Signaling cascade.…
A: The membrane potential for a neuron at equilibrium can be calculated using Nernst equation. It…
Q: You found a bright blue growth on a tomato you had left on your kitchen counter. You’ve never seen a…
A: Introduction A hypothesis is a proposed explanation for a phenomenon or observation that can be…
Q: What is shown in the graph? A) B) D) 40,000 Population exponential decay of a population exponential…
A: Growth curve: A growth curve is a graphical representation of the rate at which something grows or…
Q: What effect does stabilizing selection have on a population? The average population moves toward…
A: Stabilizing selection is a type of natural selection that favors the intermediate or average…
Q: Is there a way to diagnose an ACL issue?
A: An anterior cruciate ligament (ACL) injury is a rupture or sprain of this tough band of tissue that…
Q: Compare the lysis for bacteria to that for CHO (Chinese Hamster Ovary) cells. Note, CHO cells are…
A: Introduction :- Lysis refers to the breaking open of cells to release their contents. This can be…
Q: If the electrocardiogram looks like these, what is wrong with the heart and why?
A: An electrocardiogram (ECG) is a diagnostic tool used to record the electrical activity of the heart.…
Q: What is allosteric regulation? Describe HOW allosteric regulation acts like an “on/off” switch for…
A: The cell signaling is the communication between cells that requires specific signaling molecules…
Q: Match the pattern of inheritance to the appropriate term.
A: The above question are related to the patterns of inheritance in genetics. Genetics is the study of…
Q: Indicate next to each letter the name of the monomers shown below and write the name of the polymer…
A: Polymers are crucial in our bodies because they are involved in several biological processes.…
Q: Create a schematic diagram of the procedures below
A: Spectrophotometry It is a common method for determining the quantity of compounds in a solution or…
Q: 1. Draw and label the steps of the sliding filament mechanism in skeletal muscle. This should be…
A: Muscle contraction is a complex process that involves the interaction of numerous molecules and…
Q: True or False. DNA is positively charged. O True False
A: DNA is positively charged False
Q: provide a summarization of the following paragraph(s) in bullet points FSD is diagnosed when…
A: Female sexual dysfunction (FSD) is a prevalent condition that affects many women worldwide. It…
Q: Make an outline about the mechanism of influenza virus type c and d and explain it in detail.
A: Influenza, commonly known as the flu, is a highly contagious respiratory illness caused by influenza…
Q: What are the advantages and disadvantages of culture-dependent and culture-independent methods in…
A: Microorganisms are studied using two approaches in microbiology: culture dependent methods and…
Q: What is the importance of washing the ELISA plate
A: ELISA: ELISA (Enzyme-Linked Immunosorbent Assay) is a commonly used laboratory technique to detect…
Q: c) The extracellular matrix molecule specifically detected in the region of positive staining…
A: Introduction: The acellular stromal microenvironment of tissues is made up of an intricate network…
Q: Stetp by step for each question!
A: Introduction Phenotype refers to the observable physical or biochemical characteristics of an…
Q: Modifications of histone tails to gain access to a gene is known as ______. Chromatin…
A: Introduction :- Chromatin is the complex of DNA and proteins that make up the chromosomes within the…
Q: what would be easier to grow in a lab Paracoccus denitrificans or Methanobrevibacter smithii? Why…
A: A bacterium is a single-celled microorganism that belongs to the domain Bacteria. Bacteria are…
Q: w the polypeptide T-R-I-P at pH of 7. Label each residue with the 3-letter code, el the N- and…
A: In a polypeptide chain N terminus is formed by amino group. C terminus Is formed by carboxyl group
Q: Checkpoints during cell cycle control are important to ensure: Tumor suppression Cell…
A: A checkpoint is a control mechanism in the cell cycle that ensures the proper progression and…
Q: please describe the impact that one’s emotional state could have on food choices.
A: Food is any substance that is consumed to provide nutritional support for the body. It is typically…
Q: Nucleosomes can change the spacing between them to regulate gene expression by modifying the:…
A: Nucleosomes are fundamental units of chromatin, which is the complex of DNA and proteins that makes…
Q: Create a schemetic diagram of the experimental procedures down below (Also, attached below is a…
A: Chloroplast is the cell organelle that is found in plant cells and responsible for performing…
Q: The dot plot above shows cells that have been characterised by forward and side scatter. Forward…
A: Fluorescent dyes can be used to detect and monitor pollutants in the environment. Fluorescent dyes,…
Q: 2) Animal like protists that cannot move on their own are referred to as - www. 0 protozoans 8…
A: A protist is a group of unicellular and eukaryotic living organisms including Protozoa, Slime…
Q: Is there a way for people to improve their health by using the internet? Please explain the key…
A: Health is a state of complete physical, mental, and social well-being and not merely the absence of…
Q: If Ec=-70mV, and the membrane depolarises slightly to -60mV, how will Cl behave? O It will leak into…
A: Membrane depolarization refers to the process by which the membrane potential of a cell becomes less…
Q: Explain how Methanobrevibacter smithii, obtains energy and generates ATP; include reducing…
A: Methanobrevibacter smithii is a species of methanogenic archaea that is commonly found in the human…
Q: What does it mean to study the subcellular localization of a protein? What are two techniques that…
A: Introduction Proteins are large, complex molecules made up of long chains of amino acids. They are…
Q: A 54-year-old woman comes to the physician because of painful blisters on the trunk that appeared 3…
A: A skin condition refers to any abnormality or disease that affects the skin. The skin is the largest…
Q: What therapeutic benefits, if any, have been proposed for the psychedelic hallucinogens?
A: Psychedelic hallucinogens have been a topic of interest and controversy for many decades. These…
Q: Chapter 7 ● What types of nutrients do microbes require, and where are they obtained? What are the…
A: As per bartleby honour code ony 3 questions can be answered. Please post remaining questions…
Q: If the gene for primase were mutated so that no functional primase was produced, what would be the…
A: Bacterial DNA Replication: Bacterial DNA replication is the process by which bacteria replicate…
Q: At what temperature and what pH do most human enzymes work best?
A: An enzyme is a type of protein that acts as a biological catalyst, meaning it speeds up chemical…
Q: Pond B Pond C Figure 3. Representation of three experimental ponds located in the Hagen Aqualab at…
A: The maximum population that can exist or is tolerated in a given area depends on that area's…
Q: What does Western blot show and what is used as a control
A: Separation of individual proteins from a mixture obtained from various sources such as cells,…
Q: Many fungi are important in food production. Give three (3) examples of food made with fungi.
A: Fungi are a diverse group of eukaryotic organisms that include yeasts, molds, and mushrooms. They…
Q: What are some of the best foods to eat for digestive health?
A: There are several foods that can support digestive health and promote a healthy gut microbiome. Here…
Q: A clinical trial was carried out to test whether a new treatment affects the recovery rate of…
A: A null hypothesis is a statement that presumes no significant difference or relationship exists…
Q: Enlarge Image Tundra Taiga A biome is large regional area of similar communities characterized by a…
A: The population size depends on several factors including birth rate, death rate, immigration and…
Q: Which of the following statement about translation is accurate? a) After the DNA unwinds, both DNA…
A: Nevertheless, transcription is required before translation can take place during which RNA…
Compute for the appropriate amount (mL) of the compostions below of TE buffer used for DNA extraction.
a. 10 mM Tris
b. 1 mM EDTA
Step by step
Solved in 2 steps
- Compute for the appropriate amount (mL_) of the compositions of a CTAB NaCl solution for DNA extraction. a. 10% (w/v) CTAB b. 0.7 M NaClCompute for the appropriate amount (mL_) of the compositions of a 10 mL 2x CTAB buffer for DNA extraction. Answer D and E a. 2% w/v CTAB b. 1.4 M sodium chloride c. 100 mM Tris-Cl d. 20 mM EDTA e. 2% polyvinylpyrrolidone MW 40 000How much buffer would you need to dilute a stock solution of DNA that is at a concentration of 150ug/ml to a final concentration of 8 ng/uL in 25 ml?
- Explain how to prepare 2 ml of a solution with a concentration of 1 μg/5ml from a stock solution of a DNA sample with a concentration of 0.1 mg/ml.Explain the importance of adding TE Buffer as the final solution to isolate the concentrated DNA.You have a 50X stock solution of TAE buffer, but you need 2 L of a 1X solution for electrophoresis. How much stock should you use?
- You have a 20 mg/ml of Ethidium bromide stock solution. You need to a final concentration of 2ug/ml into a Agarose solution to visualize DNA. What is the dilution factor? Give typing answer with explanation and conclusionCalculate the concentration of DNA in your purified sample and determine the mass of the purified DNA. At 260nm the value was 1.0241........... at 280m, the value was 0.5829 Could you please explain and show the calculation of how to get the answer?a) What is the dilution factor if you add a 1ml aliquot of a specimen to 99ml of diluent? b)How to make 500ml of a 1:250 dilution?
- What is the concentration of a DNA solution with an OD260 measurement of 3.175 if 1OD unit at 260nm represents a DNA concentration of 50 g/mL? Write the correct units.Explain how gel filtration chromatography works. What type of gel will you used when the protein size is 2500 Da? Explain.Describe the function of the following reagents used in the DNA extraction procedure?a) Proteinase K b) 5M Nacl c) Isopropanol d) 1X TE Buffer