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Concentration=2.5 X 10^3 μM
OD400nm=0.859
1cm cuvette is used
what is the extinction coefficient (μM^-1 cm^-1)
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- A volumetric calcium analysis on triplicate samples of the blood serum of a patient believed to be suffering from a hyperparathyroid condition produced the following data: mmol Ca/L = 3.15, 3.25, 3.26. What is the 95% confidence interval for the mean of the data, assuming no prior information about the precision of the analysis?4.Discussed the Purposed of the Golden rules in the practice of Forensic Chrmistry.What is the practical importance of extraction in relation to chemistry especially when it comes to drugs?
- List and define the indicators that are used to assess the performance of a specific analytical method under specified conditions.After creating her standard curve for absorption versus the concentration (M) of FD&C Red 40 and processing her data, Sally found that her drinking strength Kool-Aid sample had an FD&C Red 40 concentration of 1.086 x 10–5 M. If 0.133 grams of Kool-Aid powder was used to prepare an 8-fl oz cup of her assigned flavor, what is the percent by mass of FD&C Red 40 in her Kool-aid powder?Was the gradual color change observed when the sodium thiosulfate (Na2S2O3) crystal was added to the aqueous solution of KI/12 in Station Cevidence of a chemical or physical change?
- A calibration curve of KCl conductivity (y-axis) vs concentration (mass percent, x-axis) is made. The equation of line of best fit is y = 578.3x + 0.380 and the R 2 is 0.9998. Based on this data what is the mass percent of an unknown KCl solution with a conductivity of 2,788 uS/cm? 6.55 % 6.12 % 4.14 % 3.98 % 4.82 %what is the significance of gravimetry analysis?A 1 mL sample of glycogen was calculated to contain 35 µmol (micromole) glucose. To 1 mL of this sample was added 2 mL of 2 M HCL. It was then hydrolysed by boiling the solution for 15 minutes. After boiling the hydrolysate was cooled and made up with H2O to a final volume of exactly 10 mL. The glucose was measured in this solution and found to have a concentration of 570 µg/mL (microgram/milliliter). i) Calculate the mass (mg) of glucose in the 10 mL of hydrolysate. As the 1mL of glycogen sample was made up to a final volume of 10 mL, this mass of glucose was produced by the hydrolysis of the original 1 mL glycogen sample. ii) Calculate the amount (µmol) of glucose produced by the hydrolysis of the glycogen sample. iii) Calculate the purity of the glycogen used in the sample as % Purity = (moles of measured glucose/ moles of calculated glucose in glycogen) *100 iv) state your answer in a complete sentence.
