Consider a TLC plate loaded with the following compounds: Phenyl acetate, glycine, butyric acid, and cyclohexanol a) which spots would you expect to show up under the uv lamp b) suggest the best indicator to use for each compound c) which indicator would you use to visualize all the compounds at once

Consider a TLC plate loaded with the following compounds: Phenyl acetate, glycine, butyric acid, and cyclohexanol a) which spots would you expect to show up under the uv lamp b) suggest the best indicator to use for each compound c) which indicator would you use to visualize all the compounds at once

EBK A SMALL SCALE APPROACH TO ORGANIC L

4th Edition

ISBN:9781305446021

Author:Lampman

Publisher:Lampman

Chapter89: Thin-layer Chromatography

Section: Chapter Questions

Problem 5P

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Consider the following errors that could be made when running TLC. Indicate what should be done to correct the error.a. A two-component mixture containing 1-octene and 1,4-dimethylbenzene gave only one spot with an Rf value of 0.95. The solvent used was acetone.b. A two-component mixture containing a dicarboxylic acid and tricarboxylic acid gave only one spot with an Rf value of 0.05. The solvent used was hexane.c. When a TLC plate was developed, the solvent front ran off the top of the plate.
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Use a suitable model to explain how separation and identification of a mixture of organic compounds can be achieved with a thin layer chromatographic (TLC) technique.
Upon screening with TLC (made of silica gel) chromatography using hexane/chloroform (3:1) as eluting solution, 3 spots were detected corresponding to a (A) polyphenol, a (B) tannin and an (C) alkaloid. Write the letters of the on the spots in the chromatogram shown at the right.
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Upon screening with TLC (made of silica gel) chromatography using hexane/chloroform (3:1) as eluting solution, 3 spotswere detected corresponding to a (A) polyphenol, a (B) tannin and an (C) alkaloid. If the TLC chromatogram in the picture was duplicated but using a column, draw the set-up and label approximate spots.
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