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- An ideal RF value for a compound on a developed TLC plate is 0.30. How could you adjust the TLC developing conditions to obtain an RF of 0.30 if it's too low or too high (Hint: Think about chromatography phases).How does increasing the polarity of the developing solvent affect the Rf of a compound on a silica gel TLC plate?In order to achieve the best separation of several components of different polarity in a mixture spotted on a TLC plate, what guideline should one follow regarding the choice of a developing solvent? (i.e. polar, non-polar, etc,)
- A student spots an unknown sample on a TLC plate. A single spot with an Rf of 0.55 showed up on the plate after developing the sample in hexanes-ethyl acetate 50:50. Does this prove that the unknown is a pure compound?thin layer chromatography: What would happen to the Rf value of a compound if you increased the polarity of the mobile phase while using a polar stationary phase? please help me this question! Thank you a lotThin Layer Chromatography why the non-polar compounds move up the plate most rapidly (higher Rf value), whereas polar substances travel up the TLC plate slowly or not at all (lower Rf value)? Please help me explain this question! Thank you so much!
- You will run a TLC plate after the CC placing in one spot the original sample of pigments, one spot from the yellow band and one from the green band. Draw a picture of what you expect to observe after running the TLC plate. Explain.I need to get the rf valuse from a made up value and draw a tlc plate of 30% ethyl actate and 70% peterloume etherIf a TLC was run in a solvent system that caused all the spots to show up at the baseline, how could the system be adjusted so that Rf values could be determined?
- If you run a compound on 4 different TLC plates using the following four different solvent systems which solvent system would give the largest Rf value for the compound?i need to caluclate a rf value for the tlc plate and i really need help with showing the steps please! Reduction of 4-Nitrobenzaldehyde to 4-Nitrobenzyl Alcohol and Thin Layer ChromatographyA TLC plate showed two spots A and B with Rf 0.20 and 0.30, respectively. Sketch a TLC chromatogram with the above Rf values. (Hint: Start with a 10-cm TLC plate and allow 1 cm each for the start and end solvent fronts.) Determine the distances travelled from the start of the solvent front. ___?____ A ____?___ B Determine the distances left to the end of the solvent front. ___?____ A ____?___ B