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- 5. a) Thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) can be used asexamples to explain difference between adsorption and partition. For each of these chromatographic techniques,identify:+ the stationary and mobile phase+ how the mobile phase movesb) How is the Rf value for a spot on a TLC plate calculated? What can the Rf value be used for? In order toachieve the best separation of several components of different polarity in a mixture spotted on a TLC plate,what guideline should one follow regarding the choice of a developing solvent? (i.e. polar, non-polar, etc.)1) give detail note ( giving points ) on three generation of photosensitizer (PSS) with example and also highlight the role of third generation photosensitizer . [ please refer research paper to give answer at end end of the answer please write the DOI number ]Briefly explain why the 14C/12C ratio will decrease after the death of plants. A three- to four-sentence explanation is needed.
- In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used in the mixture, the two proportions should be totalled to 1.0. but on finding You got 0.6 in total. Explain the reasons for the difference.5c)Many important bioactive compounds of plants are found in leaves and roots of a plant. Discuss the advantages and limitations of using callus cultures to produce these metabolites.4. When an organism is positive for the coagulase enzyme, which of the following would be present in the test tubes containing rabbit plasma? A. White precipitate at the bottom B. Gel plug C. Liquid D. Pellicle growth at the top
- Procedure Explant Preparation and Inoculation 1) The leaf explants were cut into squares (approximate size = 1cm x 1cm ). 2) The explants were soaked in 95% EtOH for 3 minutes with constant shaking. 3) EtOH was then decanted and 10% of Chlorox bleach was added together with a few drops of Tween 20, with constant shaking. 4) The explants were rinsed with autoclaved distilled water for at least 5 times. 5) The explants were then left to dry in the laminar air flow cabinet. 6) The explants were cultured in the appropriate media (2 explants in basal MS without BAP and the other two in MS with BAP). 7) Three explants were placed on its upper surface down and the other three with lower surface down in both basal MS media with and without BAP. 8) All the culture jars (name, date and treatments employed etc.)were labelled and incubated them (in dark) in the growth chamber or culture room. 9) Explants were placed on the surface of the medium and the Petri dish were sealed with parafilm. 10)…Help with. my research question "Does light exposure affect the neutrophils cells motility to Danio rerio?” How would I go about preparing an experiment to test?1. What results are expected if you drop H2O2 into a cut on your arm? Explain why those results are expected. 2. To do this test, why do you have to grow the organism in medium containing tryptophan? 3. Why is a red color after the addition of zinc a negative result for nitrate reductase?
- 2. Gamma-hemolysis on a blood agar plate would likely appear: Select one: a. As no change in the medium b. Blue c. Yellow d. Green e. As a clearing.It has been noticed that adulteration of palm oil with carcinogenic dyes are on the rise. Use your knowledge in analytical processes to systematically outline a protocol you will follow to process adulterated palm oil to gain qualitative and quantitative information spectroscopicall about any added toxic dye. Explain also the basis for your protocol of choice7. If a patient normally has a 12 g/dL Hb, cyanosis will likely appear when A. 10 g/dL Hb is saturated with oxygen B. 9 g/dL Hb is saturated with oxygen C. 8 g/dL Hb is saturated with oxygen D. 7 g/dL Hb is saturated with oxygen