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- 1. Give the method of sterilization of these laboratory apparatuses how they are sterilized; inoculating loop, glasswares. culture media, skin, and milk. Please cite where you got the information. Thank youDescribe the steps of the Gram stain, and explain how it can be animportant diagnostic tool for infections.1. What is the usual stain used for staining a sputum smear? 2. Give the principle of acid fast staining and gram staining.
- 1. What is the purpose for using stains? What microbial characteristics can one ascertain from a simple stain? 2. Why is it necessary to make a heat-fix smear and what are the disadvantage of heat fixing?2. Mechanical disinfection is typically more effective than manual techniques.1-The method of sterilizations we used in the tissue culture lab a. Fumigation b. Filtration C. Autoclave d. Sun light
- 2. What does a mordant do in the Gram stain procedure? Which reagent in the Gram stain is the mordant?Explain shortly the concept of differential centrifugation and how you would limit pellet contamination which is a disadvantage of this technique.4. Why is it necessary to let bacterial smears completely air dry before heat fixing?
- Explain why it is important to use only a small amount of bacteria when preparing a smear. What are the two things that are stained in a capsule stain? What is NOT stained in a capsule stain?Which of the following methods is most sensitive for identifyingdifferent strains of a microbe?a. microscopic examination b. hemolysis on blood agar c. DNA analysis d. agglutination testMatch the confirmation test to the category of bacteria. (each category can be used multiple times) Categories: -Gram Positive - Gram Negative Tests: Match with category A. Citrate test is category.... B. Indole test is category... C. Raffinose test is category... D. Catalase test is category....