Explain shortly the concept of differential centrifugation and how you would limit pellet contamination which is a disadvantage of this technique.
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Explain shortly the concept of differential centrifugation and how you would limit pellet contamination which is a disadvantage of this technique.
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- The main of objective of using 13-Line streaking method is to get: A-Contaminated cultures B-None of the above C-Overlapped colonies D-Separated colonies1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating instrument prior to and after each inoculation b. Holding the test tube caps in the hand as illustrated in Figure 1. c. Cooling the inoculating instrument prior to obtaining the inoculum d. Flaming the neck of the tubes immediately after uncapping and before recapping 2. Describe the purposes of the sub-culture procedure. 3. Explain why a straight inoculating needle is used to inoculate an agar deep tube. 4. What is the indication of bacterial growth in each of the media? (nutrient broth, nutrient agar slant, nutrient agar deep? 5. Enumerate the Good Microbiological Practices encountered in the activity. 6. Upon observation of the nutrient agar slant culture, you strongly suspect that the culture is contaminated. Outline the method you would follow to ascertain whether your suspicion is justified.discuss the significance of the different aseptic techniques utilized in maintaining sterility of the cell culture lab.
- On Aseptic Techniques: What is/are the instances/situations that each method of inoculation is to be used?during inoculation, the bacterial culture tube is always held at an angle and the lid of the Petri dish is slightly open. Explain the purpose of these steps briefly.Indicate the correct way of inoculating the following media. Plate Media For sensitivity testing ________________________________________________ For isolation of colonies ___________________________________________________
- Technique used to inoculate plate media using inoculating loop. Technique used to inoculate agar deep using inoculating needle. Pattern used to inoculate agar slant to get luxuriant culture. This pattern increases the surface area of agar that can be inoculated. Can you answer all the questions? Thankyou!1. Give the method of sterilization of these laboratory apparatuses how they are sterilized; inoculating loop, glasswares. culture media, skin, and milk. Please cite where you got the information. Thank youdiscuss why a mixed culture cannot be used to inoculate a differential media such as the tripple sugar iron agar test.
- Which of the following medium would be most appropriate for the bacterial cultures from stomach? a. LB b. Selective C. Differential d. NutientGive atleast two other examples of samples that are best prepared using Smear Preparation Technique.Take a look at your streak plate from the use of a mix culture. Does your plate show the results that are expected? If yes, what does this result tell you if no, what error do you think could’ve caused this? Explain.