Q: Why 10% bleach disinfectant are most against probiotic?
A: Disinfectant is a chemical liquid that destroys bacteria, thereby causing disinfection.
Q: (a) Which would be easier to prepare bacterial smear from, liquid or solid culture? Why? (b) Aside…
A: Introduction A bacterial smear is a small layer of bacteria that has been stained on a slide. A…
Q: Which of the following is NOT true of spread plating method? A. the maximum inoculum allowed is 1.0…
A: Please follow step 2 & 3 for detailed explanation.
Q: Do you think UVC disinfection of N95 face masks is effective? Give two important considerations for…
A: At the time of Covid-19, N95 masks are currently considered critical supplies by the centers for…
Q: n in detail gram stain biochemistry, procedures, gram-positive and gram negative cells.
A: Gram stain, a wide used microbiological, biochemical staining technique that greatly aids in the…
Q: 3. How would the lab select which antimicrobials to test against the isolated pathogen? In other…
A: There are certain criteria depending on which the lab would select which antimicrobials to test…
Q: What is the purpose of flaming an inoculating loop? How will you know when you have flamed the loop…
A: Hi, since you have posted multiple questions, we will be providing you with answers for the first…
Q: You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria…
A: The bacteria can grow only in the favourable conditions that is when there is optimum amount of pH,…
Q: There is definitely survival of some microorganisms even after alcohol treatment. Without staining…
A: Bacteria are microscopic single-celled organisms that thrive in diverse environmental conditions.…
Q: 3. Draw the specimen observed under endospore staining 4. Draw the specimen observed under…
A: Endospore staining: a. It is a differential stain used to distinguish between the vegetative cells…
Q: Why bleach disinfectant will the most effective against probiotic?
A: Probiotics are live microorganisms that have health benefits when consumed. These are usually…
Q: The sediment observed in stained blood smear caused by: a) Overstraining b) More Haematoxylin in the…
A: Here we have to explain about why sediment observed in stained blood smear.
Q: Determine the microbiology tests used to identify the microorganism(cutaneous anthrax) i. Gram…
A: Cutaneous anthrax is the most common form of anthrax infection, and it is also considered to be the…
Q: 1. Give the method of sterilization of these laboratory apparatuses how they are sterilized;…
A: Method of sterilization: Inoculating loop: Sterilized by poking it at an angle through…
Q: Describe the type of media you choose in the laboratory, use and prepare them ,, I want to describe…
A: 1. Glass slides on which the sample is placed. 2. Cover slip made up of plastic. 3. Dropper 4.…
Q: Is heat fixation used on bacterial smears that will undergo a capsule stain or flagella stain…
A: In capsule staining procedure “we do not heat fix and rinse the smear with water” as heat and water…
Q: Name 4 different sterilization/disinfecting methods, how they fight microbes,
A: STERILIZATION : It is the process in which removal of all the living forms (microorganisms) and…
Q: How does pasteurization help us in our everyday life even before it was discovered?
A: As recently as the nineteenth century, humans risked serious illness or even death by drinking…
Q: 1. Explain why the following steps are essential during sub-culturing: a. Flaming the inoculating…
A: Agar is formed by two components, agarose, and agaropectin molecules. Agar is used as culture media…
Q: 8- The following are methods of sterilization EXCEPT: O c) Sterilization by filters O b) Autoclaving…
A: Introduction: A combination of heat, chemicals, irradiation, high pressure, and filtration can be…
Q: What will happen if you heat fix ypur smear for flagella staining?
A: Falgella staining Flagella is long tail like structure present in most of the bacteria. Flagella…
Q: For each item on the following list, propose a reasonable method ofsterilization. You cannot use the…
A: There are several sterilization techniques which can be used. But, the technique to be used varies…
Q: 2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A.…
A: Hello, thank you for your question. As per our policy, I am going to answer only the first question.…
Q: Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?
A: In microscopic studies, the specimen needs to be prepared for an optimized vision under a…
Q: Transparent double-sided dishes used for growing microbes are most commonly called A) Petri dishes.…
A: The micro organisms are grown in specific containments for identification of the characteristic…
Q: 1. What is the usual stain used for staining a sputum smear? 2. Give the principle of acid fast…
A: Microorganisms are microscopic organisms that are not visible to naked eyes. Several microorganisms…
Q: 1. Define Differential Staining. 2. Application of differential staining technique.
A: "Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: 1.)What is the purpose of a counterstain?
A: BASIC INFORMATION ENZYMES They are the catalyst. They help in accelerating the chemical reaction.…
Q: 2. Mechanical disinfection is typically more effective than manual techniques.
A: Disinfection is the removal, deactivation, and killing of microorganisms. Deactivation and…
Q: 3. For each category of microbial control, list examples. Sterilization Disinfection Sanitization/…
A: All of these are methods are used to prevent infection by slowing or stopping the growth and spread…
Q: What is the usual stain used for staining a sputum smear?
A: Since you have asked multiple questions, we will answer only first question for you. In order to get…
Q: 3. Based on his findings at the farm, the USDA inspector was also conçerned that the milk could be…
A: The E. coli O157:H7 is the bacterial species responsible for causing disease which typically…
Q: 4. Use the words presented in below to create a concept map illustrating the relationships among…
A: All these terms mentioned in the question, are related to the isolation, cultivation, and…
Q: Are TSA, MRS and MOX differential media? Why or why not?
A: In microbial biology, differential media refers to any growth media that comprises of certain…
Q: 1. What is the purpose for using stains? What microbial characteristics can one ascertain from a…
A: NOTE: AS PER OUR GUIDELINES WE ARE ONLY ALLOWED TO ANSWER FIRST QUESTION. PLEASE RESUBMIT THE OTHER…
Q: Is UV treament more or less effective than disinfection when used against Saphylococcus aureus?
A: Saphylococcus aureus is a gram-positive bacterium that is found in the human nose, skin, and…
Q: Do you think UVC disinfection of N95 face masks is effective? Give two (2) important considerations…
A: N95 filtering facepiece respirator or N95 mask is a piece of personal protective equipment that…
Q: In spread plate method, inoculating loop is used to spread bacteria from the inoculation site. For…
A:
Q: Bacterial Endospores 7. List the major steps, in sequence, needed to perform the…
A: Bacterial endospores:When there are normal environmental conditions and nutrient availability…
Q: Describe the bacteria observed in different staining procedures. Please refer to the photo - Direct…
A: The cell wall of most bacteria has an overall net negative charge and thus can be stained directly…
Q: Benefits of UV light sanitizer spray gun
A: Spray gun can be of two types. First type of spray gun that spray out wet mist while the second type…
Q: In Gram staining method, list the reagents and state their role for each step of the procedure? In…
A: Bacteria are unicellular, prokaryotic organicism which are devoid of membrane bound organelles and a…
Q: 1) would you describe the contents of the soil-inoculated broth as being a “pure culture”? Why or…
A: 1. A soil-inoculated broth cannot be considered as a “pure culture”. A pure culture is a collection…
Q: Why might gram positive bacteria incorrectly appear to be gram negative after the gram staining…
A: Gram staining is a procedure which is used to identify whether the bacteria being tested in gram…
Q: What is the purpose for using stains? What microbial characteristics can one ascertain from a simple…
A: Microorganisms are the tiny organism that cannot be visualised by naked eyes therefore microscopy is…
Q: Which of the following is used in the sterilization of the medium? a) benmarge b) swab c) autoclave…
A: Sterilization is the process of making something (instrument, swab, room etc. ) free of microbes…
Q: 2. Give the purpose of each of the following reagents in a differential staining procedure: (Primary…
A: Differential Staining uses multiple chemical stains to view the microscopic organisms. It is because…
Q: During the preparation of a smear leading into simple staining of the bacterial culture S.…
A: S. epidermidis is a gram-positive bacteria.
Q: What is the most common sterilization technique used in laboratories?
A: According to the guidelines we have to answer only first question, rest you can ask separately thank…
4. Why is it necessary to let bacterial smears completely air dry before heat fixing?
Step by step
Solved in 2 steps
- 1. What is the purpose for using stains? What microbial characteristics can one ascertain from a simple stain? 2. Why is it necessary to make a heat-fix smear and what are the disadvantage of heat fixing?1. What is the significance of soaking the container in bleach? 2. What is the significance of keping the container closed except when pouring the medium; partially opening the container during pour-plating in preparing culture media?7.Why is it necessary to make a skin puncture to a depth of 3mm?
- 1. What is the usual stain used for staining a sputum smear? 2. Give the principle of acid fast staining and gram staining.1. Give the method of sterilization of these laboratory apparatuses how they are sterilized; inoculating loop, glasswares. culture media, skin, and milk. Please cite where you got the information. Thank you1. What is the hazard of the spattering tendency in heating a wire loop with specimens over a flame? 2. Why is heat a highly effective sterilizing agent?3. Which of the three methods of heat sterilization is the most appropriate and practical to use in sterilizing antibiotic solutions? (Direct flame, Dry heat, Moist heat)
- 1. Differentiate between Gram positive and Gram negative cells in smears. 2. Discuss how to properly prepare a bacterial smear.1.)What is the purpose of a counterstain? 2. What does a mordant do in the Gram stain procedure? Which reagent in the Gram stain is the mordant? 3. True or False? The oil objective should make contact with the oil on the slide. 4. Why is it necessary to let bacterial smears completely air dry before heat fixing? 5.Why should controls be included wherever possible for any staining technique? 6. Why is it necessary to heat the slides while staining for endospores?1-The method of sterilizations we used in the tissue culture lab a. Fumigation b. Filtration C. Autoclave d. Sun light