Q: n in detail gram stain biochemistry, procedures, gram-positive and gram negative cells.
A: Gram stain, a wide used microbiological, biochemical staining technique that greatly aids in the…
Q: what color would you expect for a gram positive microorganism for each incorrect mistakes while…
A: "Gram staining" is a technique used in microbiology. It is a method in which a bacterium can be…
Q: 3. Draw the specimen observed under endospore staining 4. Draw the specimen observed under…
A: Endospore staining: a. It is a differential stain used to distinguish between the vegetative cells…
Q: What might happen if the Gram staining procedure is performed ona culture incubated for a little…
A: Gram staining is one of the staining methods used in laboratories to differentiate between two broad…
Q: A capsule stain is kind of: A Simple and Negative Stain B Differential and negative stain C…
A: Introduction Capsule is the outer most covering, which is present even outside the cell wall. This…
Q: I need to discribe the "the colors of gram+ and gram (-) bacteria" in each independent step of the…
A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…
Q: . In preparing a slide, what is the significance of heating the sample after it is stained?
A: 2.Fixation is often achieved either by heating (heat fixing) or chemically treating the specimen. In…
Q: . What is the purpose of fixation in cytological staining?
A: Tissue fixation is a critical step in the preparation of histological sections for staining…
Q: Why is it necessary to use a 24-hrold culture in gram staining?
A: Note: Since you have posted multiple independent questions in the same request, we will solve the…
Q: 1. What is the hazard of the spattering tendency in heating a wire loop with specimens over a flame?…
A: “Since you have posted a question with multiple sub-parts, we will solve first three sub parts for…
Q: 2. Explain the relationship between bacterial cell wall with gram positive and gram positive in the…
A: Gram staining is the procedure that is used to differentiate between the gram positive and gram…
Q: What features of your Gram stain exemplify the Gram stain theory?
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: Notes on Mann's methyl -blue eosin stain
A: Staining is a technique that is used in microscopy to visualize the cells and cell components in a…
Q: 1. a) What are Romanowsky stains? Name four of these. b: What are the components of a Romanowsky…
A: 1) a. Romanowsky staining is a prototypical staining technique which is also known as…
Q: You perform a Gram stain. Your Gram positive control, your Gram negative control, and your sample…
A: Gram-staining is a technique used to classify bacterial species, either as gram-positive or…
Q: I Why does't a negative stain colorize the cells in the smear?
A: Staining is a technique which is used for enhancement of contrast in samples mostly at the level of…
Q: -What are the steps of gram staining -Which of the four steps is the most important and why? - What…
A: Based on the chemical and physical characteristics of their cell walls, the Gram stain is an…
Q: The Gram Stain is able to stain bacterial cells differently depending upon the structure of their…
A: Bacterial cell wall is made up of peptidoglycan. it prevents the cell from bursting when it absorbs…
Q: What does a mordant do in the Gram stain procedure? Which reagent in the Gram stain is the mordant?
A: Gram staining is a procedure which is used to classify the bacterial cells on the basis of their…
Q: What would be the result of the gram stain if acid alcohol is used as a decolorizer rather than the…
A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…
Q: 1. Define Differential Staining. 2. Application of differential staining technique.
A: "Since you have asked multiple questions, we will solve the first question for you. If you want any…
Q: 1.)What is the purpose of a counterstain?
A: BASIC INFORMATION ENZYMES They are the catalyst. They help in accelerating the chemical reaction.…
Q: Solid media is usually prepared with a 1-2% final concentration of agar. If these plates were…
A: Solid media is the type of media that is made up of liquid substances like distilled water and other…
Q: What is the usual stain used for staining a sputum smear?
A: Since you have asked multiple questions, we will answer only first question for you. In order to get…
Q: What is the purpose of iodine in the Gram stain? To act as a counterstain None of the other…
A: There are various staining techniques that are used to stain different cellular structures. The Gram…
Q: - After you are finished, list each step for the Gram staining procedure. - Which of the four steps…
A: NOTE: AS PER OUR GUIDELINES WE NEED TO ONLY ATTEMPT FIRST QUESTION AND ITS PARTS , PLEASE RESUBMIT…
Q: Using the acid-fast stain's name as a guide, what would be a logical alternative name for the Gram-…
A: Gram staining is a common technique used to differentiate two large groups of bacteria based on…
Q: Staining bacterial capsule can not be accomplished by?
A: Since most standard stains cannot enter the capsule because it is so tightly packed, it is difficult…
Q: 4. The Gram stain, if the decolorizing step is deleted, gram-negative cells will appear at the…
A: Decoloriser in gram staining method is to dehydrate the peptidoglycan layer of the cell wall causing…
Q: Discuss the various theories on the Gram stain.
A: Bacteria can be classified into two major types according to gram staining: Gram positive. Gram…
Q: 1. What is the purpose for using stains? What microbial characteristics can one ascertain from a…
A: NOTE: AS PER OUR GUIDELINES WE ARE ONLY ALLOWED TO ANSWER FIRST QUESTION. PLEASE RESUBMIT THE OTHER…
Q: 7. Draw what you would expect to see for acid fast stain and endospore stain procedure if you did…
A: Staining is a technique for adding color to cells, tissues, or microscopic components so that they…
Q: 6. What is the function of the iodine solution in the Gram stain? If it were omitted, how would…
A: Gram stain is a technique used for staining bacteria in tissues.
Q: 4. Make drawings of your simple stain preparations. Bacillus sp. Staphylococcus sp. Shape Shape…
A: In microbiology the characteristics of the microorganisms is very important. For that purpose…
Q: 1-What microbial characteristics can one ascertain from a simplest stain? 2-A student is directed…
A: Gram staining method: This technique is useful in identifying two groups of bacteria based on the…
Q: What is the purpose for using stains? What microbial characteristics can one ascertain from a simple…
A: Microorganisms are the tiny organism that cannot be visualised by naked eyes therefore microscopy is…
Q: Which of the following acts as the decolorizer in the gram staining? A. Safranine B. Iodine C.…
A: Gram staining is a staining technique used to differentiate two large groups of bacteria based on…
Q: 2. Give the purpose of each of the following reagents in a differential staining procedure: (Primary…
A: Differential Staining uses multiple chemical stains to view the microscopic organisms. It is because…
Q: k there are 2 staining reagents applied in differential s
A: Differential Staining- It is a staining process which uses more than one staining reagent. it is…
Q: 1. What is one advantage of utilizing the pour plate technique over the streak plate technique ?
A: Answer: THERE ARE THREE TYPES OF CULTURE METHODS 1. POUR PLATE TECHNIQUE 2. STREAK PLATE TECHNIQUE…
Q: Describe how the Gram stain is performed and identify the staining characteristics of Gram(+) and…
A: Gram staining technique is majorly used to classify the two wide groups of bacteria. This technique…
Q: in gram staining: what had happen when the following were applied: Dye, Mordant, Decoloriser,…
A: The bacteria are classified into two categories depending on the nature of cell wall. they are…
Q: 1. What is the significance of soaking the container in bleach? 2. What is the significance of…
A: Significance of container soaking in bleach before pouring :-
Q: What are the principle and basic concepts of GRAM STAIN?
A: Gram staining is among the most important microbiology staining procedures. It was first invented in…
Q: 2. Explain the relationship between bacterial cell wall with gram positive and gram negative in the…
A: Gram staining experiment helps to identify the gram-positive and gram-negative bacteria, and it…
Q: .) What is the purpose of acid-fast staining? What information can you gain from this procedure?…
A: Acid fasr staining is the differential staining method which was first developed by Ziehl and…
Q: ) Outline or discuss what happens to a gram negative and gram positive cell at EACH Individual step…
A: On the basis of lipid content and peptidoglycan layer in the cell wall, bacterias are classified…
1. What is the usual stain used for staining a sputum smear?
2. Give the principle of acid fast
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- 1.)What is the purpose of a counterstain? 2. What does a mordant do in the Gram stain procedure? Which reagent in the Gram stain is the mordant? 3. True or False? The oil objective should make contact with the oil on the slide. 4. Why is it necessary to let bacterial smears completely air dry before heat fixing? 5.Why should controls be included wherever possible for any staining technique? 6. Why is it necessary to heat the slides while staining for endospores?1. What is the purpose for using stains? What microbial characteristics can one ascertain from a simple stain? 2. Why is it necessary to make a heat-fix smear and what are the disadvantage of heat fixing?2. What does a mordant do in the Gram stain procedure? Which reagent in the Gram stain is the mordant?
- 3. Draw the specimen observed under endospore staining 4. Draw the specimen observed under positive simple staining1. What is the usual stain used for staining a sputum smear? 2. Give the principle of acid fast staining and gram staining. 3. Explain the Bethesda System. 4. How will you instruct a patient that will undergo a brochoscopy?1. What is the purpose for using stains? What microbial characteristics can one ascertain from a simple stain? 2. Why is it necessary to make a heat-fix smear and what are the disadvantages of heat fixing? 3. what is the best age for your culture when performing a gram stain? Why? 4. Why is Gean staining considered a differential staining process? 5. What are some reasons a Gram positive cell might appear Gram negative?
- 1. Define Differential Staining. 2. Application of differential staining technique.1.Procedure for sectioning paraffin blocks 2.Mann's methyl blue eosin stain?1. a) What are Romanowsky stains? Name four of these. b: What are the components of a Romanowsky stain?b: What is the optimum pH for staining with Giemsa. c: Name two conditions which will affect the quality of your staining. d: What is a panoptic stain. Give one example. e: Name two blood parasites that can be demonstrated using the Giemsa stain.
- Give the principle of acid fast staining and gram staining.1-What microbial characteristics can one ascertain from a simplest stain? 2-A student is directed to make a simple stain of coli with crystal violet, but got mixed up and used safranin instead. How would their observations be different? Would the information obtained be any different? 3-How is the procedure different when taking cells from a solid medium compared to taking cells from a liquid medium? Why is it important that your smear be thin?Hi , First Test Gram stain is Gram negative, and in order to find that what other possible test can be performed ? For example - Oxidation Test for test 2. . And for 3,4,5 and 6 ? What other possible test can be performed? Thank you !