digest suspect B's DNA using restriction enzyme 1. Restriction enzyme 1 cleaves the recognition site 5' -AA/TT -3' . And count the fragment sizes from the digestion of the suspect.

Q: Define the following terms:a. hypochromic effectb. DNA denaturationc. restriction endonucleasesd.…

A: Recombinant DNA is made by combining two or more DNA from other sources. The process depends on the…

Q: In recombinant DNA technology, restriction enzymes cleave the DNA at a specific site known as:

A: Restriction enzyme, also known as restriction endonuclease, is a bacterial protein that cleaves DNA…

Q: A ____________ is required to transfer DNA sequences from one organism to another. reporter gene…

A: A reporter gene is a gene that is used as a selectable marker in gene cloning to identify whether a…

Q: Enzymes of bacterial origin used in a wide variety of techniques are: ligases restriction…

A: DNA polymerase

Q: a. How do you set up the tanks? b. Why do DNA fragments move? C. How do DNA fragments move relative…

A: There are multiple questions in this particular question, I will answer the first three sub parts…

Q: Draw an Illustration of the steps in restriction digestion and PCR [ Please make it clean and…

A: Restriction digestion is the process of cutting the DNA sequence at a particular nucleotide sequence…

Q: Based on the restriction enzyme specificities given below, what was the enzyme utilized to produce…

A: An enzyme that recognizes a specific sequence on the DNA strand and cuts the DNA into fragments is…

Q: etion enzyme Sall cuts the sequence GTCGAC to leave a five base, 5' overhang. The restriction enzyme…

A: A restriction enzyme is classified under the category of a protein. These are isolated from bacteria…

Q: DEFINE THE FOLLOWING: 1) restriction enzyme 2) plasmid 3) recombinant DNA

A: Ans 1) Restriction enzymes are enzymes that are isolated from bacteria that can cleave…

Q: 1. Restriction enzymes can occasionally cut at site(s) other than its restriction site. 2. DNA…

A: NOTE:- As you have posted multiple questions, we will solve first two parts for you, To get the…

Q: Define the following terms: Plasmid Restriction Enzyme Standard Curve

A: Recombinant DNA technology is similar to gene cloning that makes use of restriction enzymes to cut…

Q: Restriction enzyme:   Recognition sequence:     Number of pieces of DNA:  Type of cut:

A: In the question a DNA sequence is given with its nucleotides.  Restriction enzyme is an enzyme that…

Q: Which of the restriction enzymes listed in the table below produces blunt-end fragments? Enzyme…

A: The blunt end are produced by the restriction enzymes when the end of a DNA fragment  after breaking…

Q: The detection of single nucleotide polymorphisms is possible with: O A. Restriction Enzymes O B. All…

A: SNPs or single nucleotide polymorphisms are the most common type of genetic variation in humans.…

Q: Restriction digestion of DNA fragments is not sequence specific. True False

A: Within a few years after discovering EcoB, EcoK, and HindII, scientists were already experimenting…

Q: Select the statement below that is FALSE regarding DNA and DNA Fingerprinting: Select an answer and…

A: Each individual is unique because of his DNA sequences.  Determining the DNA sequences by cutting…

Q: In 5 sentences only, What are restriction enzymes (RE)? Describe how a RE can be used to…

A: Researchers continue to find new medications that aid in the treatment of deadly diseases and enable…

Q: Using the chart posted to the left of the microwave in lab, what is the 3. restriction recognition…

A: Step 1 In 1963, Arber discovered that Escherichia coli is able to protect itself from the attack of…

Q: Based on the restriction enzyme specificities given below, what was the enzyme utilized to produce…

A: The corner stone of genetic engineering or recombinant DNA technology is a special class of enzymes…

Q: Features of restriction enzymes include the following, except  __________ . a. They are produced…

A: Restriction enzyme is a group of enzymes which involves the cleaving of DNA molecule at a specific…

Q: following site does the restriction enzymes act? 32. Restriction Fragment Length Polymorphism (RFLP)…

A: Restriction endonucleases are enzymes that cuts the DNA at specific DNA sequence known as…

Q: You are studying a protein that contains the peptide sequence RDGSWKLVI. The part of the DNA…

A: The restriction endonuclease are responsible for cutting the DNA at specific sequence and they…

Q: Mention two classes of restriction enzymes. Suggest their respective roles.

A: The enzyme mainly involved in recombinant DNA technology are restriction enzymes, ligases, lyases,…

Q: Select all the following statements that are correct regarding agarose gel electrophoresis and…

A: Agarose gel electrophoresis is a process used for separation of DNA fragments. Steps of agarose gel…

Q: The recognition site for Spel is A'CTAGT. Which of the following restriction sites when digested…

A: Restriction site - Each restriction enzyme identify specific sequence of nucleotide, known as…

Q: There are three classes of restriction enzymes; Class I, Class II and Class II. Nevertheless, Class…

A: Restriction endonuclease are the enzyme which make a cut in genetic material that is DNA somewhere…

Q: Suppose that a geneticist discovers a new restriction enzyme in the bacterium Aeromonas ranidae.…

A: Restriction enzymes or restriction endonucleases are the enzymes that cut the DNA strand at specific…

Q: Which of the following can be termed as a restriction modification system?a) Restriction…

A: Enzymes are the biological catalysts which are proteinaceous in nature. The function of an enzyme is…

Q: You want to insert a sequence in the lacI gene to alter its function,what restriction enzyme(s) will…

A: The lacI gene is a part of the lac operon, that codes for a protein that inhibits the expression of…

Q: Describe the purpose and process of DNA finger printing.

A: DNA fingerprinting is the method of recognizing the genetic makeup of individuals through the…

Q: Technique whereby inserting DNA into a clone is accomplished using two different restriction enzymes

A: Directional cloning is the technique which is accomplished by cleaving the plasmid vector with two…

Q: Which of the DNA sequences shown below can be cut by using restriction enzyme' Explain your answer…

A:

Q: When using blunt-end primers, how would you determine the correct plasmid construct from the…

A: Ligation is the joining or ligation of two nucleic acid fragments through the action of a ligase…

Q: DNA samples from four individuals were cleaved with the same MW restriction endonuclease. The DNA…

A: Any human biological specimen taken or preserved for the purpose of extracting and analysing DNA in…

Q: A DNA fingerprint consists of sections of DNA that have been cut with restriction endonuclease…

A: DNA fingerprinting DNA fingerprinting is a modern technique that help us to solve  crimes. The…

Q: Tailed primer can have restriction enzyme sites. True False

A:

Q: If the Hinfl restriction enzyme recognizes G^ACTC sites, as seen in the image, belUW. GACTC How many…

A: The cloning of DNA is the mechanism by which a certain fragment of DNA is multiplied. The selected…

Q: Please determine the relative location of the three restriction enzymes. Enzyme Number of fragments…

A: Restriction enzymes are a type of enzyme that can detect and cleave a certain short sequence of…

Q: Suppose that a geneticist discovers a new restriction enzyme in the bacterium Aeromonas ranidae.…

A: Restriction enzymes are found in bacteria, their function is to recognise and cut a specific site on…

Q: A circular DNA molecule is subjected to complete restriction digestion by (1) BamHI alone, (2)…

A: Restriction enzymes cut DNA at a specific location called as restriction site.

Q: The addition of restriction endonucleases in the cloning process is done following the ligation with…

A: DNA cloning is a technique that involves making of clones of genes from particular gene of interest…

Q: Identify which mutagen is described by the following statement. Results to the deamination of…

A: NOTE: The options are numbered as 1, 2, 3, 4, 5, and 6.  DNA(deoxyribonucleic acid) is the basic…

Q: triction enzyme digests DNA into fragments.term the technique used to check the progression of this…

A: Living cells include nucleic acids such as DNA and RNA. Almost all live cells have both RNA and DNA,…

Q: b) Describe how DNA is digested by different restriction enzymes c) Describe how gel electrophoresis…

A: DNA digestion is the process in molecular biology which is done by restriction enzymes for the…

Q: A linear DNA fragment was produced by digestion with the restriction enzyme, Xba1. This fragment…

A: DNA or deoxyribonucleic acid is a polymer of deoxyribonucleotides connected together via…

Q: what restriction enzymes could be used to cut cDNA produced ?

A: DNA-cutting enzymes are restriction enzymes. Every enzyme detects a single or some few target…

Q: RNA primer is removed from the Okazaki fragment bya) DNA polymerase Ib) DNA polymerase IIc) DNA…

A: Ans: DNA polymerase I is responsible for the removal of RNA primer from the okazaki fragment.

Q: Restriction mapping of a linear piece of DNA reveals the following EcoRI restriction sites. EcoRI…

A: Introduction By cleaving the internal covalent bonds between nucleotides, endonucleases breaks down…

Crime Scene Investigation

A crime scene refers to a place where a crime has occurred. The purpose of crime scene investigation (CSI) is to analyze the complete scene and catch the culprit based on the pieces of evidence collected from the place of crime. The people involved in this process are referred to as crime scene investigators.