1. Restriction enzymes can occasionally cut at site(s) other than its restriction site. 2. DNA ligase is used to form hydrogen bond between complementary strands.
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- In 5 sentences only, What are restriction enzymes (RE)? Describe how a RE can be used to develop/design a DNAmarker.Explain briefly within 5 sentences each 1. a. What is PCR? Explain/relate its importance in DNA marker analysis. 1.b. What are restriction enzymes (RE)? Describe how a RE can be used to develop/design a DNAmarker.1. (a) Restriction sites are usually ______. Recombinant DNA Technology Restriction enzymes Ligase Palindromic sequences (b) Involves joining a donor DNA fragment of interest to a vector Recombinant DNA Technology Restriction enzymes Ligase Palindromic sequences
- Draw an Illustration of the steps in restriction digestion and PCR [ Please make it clean and readable, labelled should be included, Drawing ] ( THIS IS ALL ABOUT APPLICATIONS OF RECOMBINANT DNA).Definition of Terms( This is all about Applications of Recombinant DNA) a. Clone b. Plasmids c. Biotechnology d. PCR Amplification e. Detection f. Modified Trait g. Human Genome h. Genetic Modified OrganismIllustrate the steps in restriction digestion and PCR [ Please make it clean and readable, labelled should be included ] ( THIS IS ALL ABOUT APPLICATIONS OF RECOMBINANT DNA).
- Features of restriction enzymes include the following, except __________ . a. They are produced by both prokaryotes and eukaryotesb. may be type IIc. make single and double stranded breaks in DNAd. operate at a single temperaturee. Are used in nature to destroy bacteriophage DNAChromosomal DNA fragments for molecular cloning are cut by _________, and inserted into molecular vectors (such as plasmids) using __________. a. Ligase; Restriction Endonucleases. b. Restriction endonucleases; Ligase. c. DNA polymerase 1; Primase. d. DNA polymerase 1; Ligase. e. Primase; Helicase.Give me 3 examples of how PCR and restriction enzymes can be used to create a genetic fingerprint
- PCR can be used______ . a. as a cloning vector b. in DNA profiling c. to modify a human genomeDefinition of Terms( This is all about Applications of Recombinant DNA){ 2-3 sentences only) a. Clone b. Plasmids c. Biotechnology a. PCR Amplification b. Detection c. Modified Trait d. Human Genome e. Genetic Modified OrganismA more modern molecular technique to RFLP fingerprinting is called Amplified Fragment Length Polymorphisms (AFLPs). In AFLP analysis, restriction enzymes are again used to digest genomic DNA into multiple fragments. Next, adapters complementary to restriction site overhangs are ligated to the fragments using an enzyme called DNA ligase. These adapters are complementary to primers used to amplify the fragments using the polymerase chain reaction (PCR). Can you think of any potential benefits of AFLP analysis over RFLP? Explain your reasoning.