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Two dyes were
placed in an agar gel. Which dye migrated through the
gel faster? Why? Explain in brief simply terms
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- if the absorbance value measured in a spectrophotometer is 0.5, how much of the original incident light is being measured by the detector?Consider a bi convex lens with radius of curvatures |R1| = 30 mm and |R2| = 180 mm, ct = 5 mm and glass is N-BK7. This is used as camera lens in order to image a man who is at 1 km away from the camera. We want to resolve 10 cm on his body. Assume that λ = 550 nm. Using zemax, determine the MTF value for the resolution of R = 80 lp / mm in image plane which is placed at paraxial focus. (Hint: First, find the diameter of the lens) Which one: a.32.1 % b.28.7 % c.12.3 % d.58.5 % e.46.8 % f.57.2 % g.20.9 % h.15.6 %Enter the correct color for the visible light for each wavelength. 380-450 450-495 495-570 570-590 590-620 620-750 From the above wavelengths, which has the highest amount of energy?
- Normal room temperature is about 74 degree. What is the equivalent temperature on the Celsius scale?The magnesium electron spectrum has a line at 266.8 nm. Does it have a greater speed in a vacuum (an area without an atom or particle) than does red light with a wavelength of 652 nm? Why or why not?Microscopic objects often are measured in micrometers. A micrometer equals1/1000 of a millimeter and is symbolized by μm. What is the diameter of thescanning power field of view in micrometers? ___________
- A solution containing 2g/liter of a light-absorbance substance in 1 cm cuvette transmits 75% of incident light of a certain wavelength. Calculate the transmission of a solution containing 4g / literWhat are the light sources used in UV-vis spectrophotometry.give ansA michroscope has a 20x ocular and two objectives of 10x and 43x respectively: calculate the apparent magnification for low and high power lenses.
- Would it be more difficult to meet a 70 ppb one hour averaged standard or 70 ppb 8-hr averaged standard and why?Compute the total magnification. [Use 10x magnification for eyepiece]1. Scanning2. LPO3. HPO4. OIOWhat color does an object appear if it absorbs light at both 420-500 nm and at 620-700 nm? a. Red b. Black c. Purple d. Green e. Deep blue