Distinguish between simple diffusion (SD), facilitated diffusion (FD), and active transport (AT) across a membrane for the following questions. (More than one may be tru (a) Which processes are energy dependent? (b) Which processes need some kind of carrier protein(s)? (c) Which processes can be saturated by substrate? gradient?
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- For each type of membrane transport, know the following:– Is a transporter protein required? If so, what type?– Is there an energy requirement, and if so, what is the energy source?– What is the relative rate of solute transport based on molecule type? On concentration gradient?– What are examples of the types of solutes transported by carriers and channels?Discuss carrier-mediated transport. How could you experimentally distinguish between the different types of carrier-mediated transport?Ouabain is a specific inhibitor of the active transport of sodium ions out of the cell and is therefore a valuable tool in studies of membrane transport mechanisms. Which of the following processes in your own body would you expect to be sensitive to inhibition by ouabain? Explain your answer in each case. a) Facilitated diffusion of glucose into a muscle cell b) Active transport of dietary phenylalanine across the intestinal mucosa c) Uptake of potassium ions by red blood cells d) Active uptake of lactose by the bacteria in your intestine
- What do facilitated diffusion and active transport have in common? What are the differences between them?Typically, there is an ion gradient between the cytoplasm and the interior of synaptic vesicles. The ion gradient has a functional role. Which of the following statements best describes the ion gradient across the synaptic vesicle membrane and its role? a.) There is a proton gradient across the synaptic vesicle membrane, and it provides the driving force for the transport of neurotransmitter into the vesicle. b.) There is a sodium gradient across the synaptic vesicle membrane, and it provides the driving force for the transport of neurotransmitter into the vesicle. c.) There is a proton gradient across the synaptic vesicle membrane, and it provides the driving force for the transport of neurotransmitter out of the vesicle. d.) There is a sodium gradient across the synaptic vesicle membrane, and it provides the driving force for the transport of neurotransmitter out of the vesicle.Uniporters and ion channels support facilitated transport across cellular membranes. Although both are examples of facilitated transport, the rates of ion movement via an ion channel are roughly 104- to 105-fold faster than the rates of molecule movement via a uniporter. What key mechanistic difference results in this large difference in transport rate? What contribution to free energy (ΔG) determines the direction of transport?
- Which of the following membrane transport mechanism requires both the aid of proteins and the expenditure of cellular energy?Gated transport occurs in which of the following pathways? A: from the cytosol to ______? Endoplasmic reticulum or mitochondria or cell nucleus etc?During an investigation on membrane transport, a researcher exposed bacterial cells to different concentrations of two different solutes: A and B. The rate of transport of each solute into cells isrepresented in the graphSolute ASolute BSolute ConcentrationWhich of the following best explains the greater rate of transport for solute A than for solute B at higher solute concentrations?A Solute A is being transported by simple diffusion, which does not rely on membrane proteins to control the rate of transportSolute A is being transported by active transport, which uses ATP and has higher rates of transport than passive transportSolute A is being transported by facilitated diffusion, which uses membrane proteins to increase the rate of transportRate of Transport
- In primary/direct active transport, does ATP always have to be involved? Definitions seem to say the movement must be coupled with an exergonic reaction. Wouldn't the movement of a solute down its concentration gradient be exergonic, or is this an incorrect statement?What is the difference between simple and facilitated diffusion? Facilitated by which type of molecule does the term “facilitated” mean?For the following modes of transport for membranes, tell whether the flow is with or against chemical potenial. Meaning +deltaG or -deltag A. Passive Diffusion B.Faciliated Diffusion C.Active Transport