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- PCR is a new molecular technique used for a variety of purposes. Which of the following statements is true about PCR?a. It can be used for genotyping.b. It can be used for paternity testing.c. It can be used for cloning. Group of answer choices A only All of the above are correct C only B onlyA) What are the three main steps involved in PCR? Include temperatures and descriptions of each step. B) Explain why RFLP can produce many bands on an electrophoresis gel and PCR (one set of primers) will only produce one or two bands on a gel for the same genome.Regarding the PCR technique, what is false?a. It can produce multiple copies of DNA.b. It is the same as DNA fingerprinting.c. It is not a time-consuming process.d. It cannot successfully copy whole genes
- In another experiment, Griffith mixed heat killed S strain with live R strain bacteria and injected the mixture into mice. A. What strain of the bacteria was found in the blood samples of the mice? B. What were the results of this experiment? C. What conclusion did he reach based on these results?When constructing a recombinant DNA molecule, a marker gene is used to: a. give the organism a new trait, such as insect resistance b. Identify whether the transformed organism contains the recombinant DNA c. replicate (copy) the gene of interest d. Introduce the recombinant DNA into an organism e. cut short sequences of DNAPCR can be utilized _______ a. as a cloning vector b. to modify a human genome c. all of the choices d. in DNA profiling
- What would be the outcome if the primers used in a polymerase chain reaction have lower GC content (<40 %), shorter, and more variable than the intended oligonucleotide sequence? a.The PCR reaction will cease after the first cycle b.The reaction will yield a mixture of non-specific products. c.All of these d.The PCR reaction will not start. e.The reaction will yield a single short PCR product.The results of the treatment of 20 boys with SCID-X1 showed that ____. a. the “treatment” caused numerous unforeseen complications. b. a bone marrow transplant can cure SCID-X1 c. SCID-X1 can be cured through genetic engineering d. none of those treated were cured of the disease e. our understanding of the human genome exceeds our ability to modify itWhich of the following would be considered a transgenic organism? A. a human whose human blood-clotting gene was silenced B. a fern grown in cell culture from a single fern root cell C. a rat with rabbit hemoglobin genes
- A. What is the % of GMO corn, sugar beets and soybean grown in the US? B. . Differentiate between GMOs and Genetically Engineered (aka Transgenics) Organisms? C. Brassica oleracea is the wild parent of what types of agricultural crops? D. What are RoundUp Ready (RR) crops?All are true about PCR except A. Automated PCR machine called a thermal cycler B. A thermostable DNA polymerase is required C. Primer extension occurs at 72˚C D. Annealing involves binding of primer between 90-98˚CWhich of the following is true? a. Viruses have been used to introduce certain genes into humans. b. PCR can be used in tests for inherited diseases c. All the above d. DNA chips can carry sequences from over 1000 gene sequences on one chip.