EcoRI 4359Aatll - Zral 4284Bei 4209BsrBl 4205Clal - BspDI 23Hindill 29EcoRV 185Bmtl - Nhel 229Sspl 4168Earl 4155Acul 4048Xmnl 3961Hincll 3905Scal 3844BamH 375Sgrl 409Banll 471Banll 485Вы 3787Bsl 3759Bgl 528Sphl 562EcoNI 622Sal - Acct - Hincll 651Pvul 3733Pstl 3607Bartl 3602Pshl 712Asel 3537Eagl 909Bell 949Bsal 3433BarDI 3420Nrul 972PBR3224,361 bpBstAPI 1045Ahdi 3361BspMI - Bfual 1063PAMI 1315Bsml 1353PAMI 1364Acul 3000OriStyl 1369Aval - BsoBl 1425PpuMI 1438Msel 1444Bigl 1447Ppu 1480AlwNI 2884Bell 2777kI 2682ropDrdi 2575Bsgl 1650BspEI 1664Pcil - Afl 2473rBl 2404Earl 2351Bspol - Sapl 2350Ndel 2295BstAPI 2291Bsaß 1668Xmat 2029Pvull 2064BsmBI 2122Dndl 2162BstZ171 - Acct 2244Bsal 2225TthI- PI 2217Figure 1If your gene of interest was inserted at the Sphl restriction site of the plasmid illustrated inFigure 1, describe the screening process to select the positive recombinants.

SCREENING OF POSITIVE RECOMBINANTS Since the gene of interest is inserted at the Sph-1 site, the…

EcoRI 4359 Clal - BspDI 23 Hindi 29 Aatll - Zral 4284 Bki 4209 Borl 4205 EcoRV 185 Sspl 4168 Earl 4155 Acul 4048 Xmnl 3961 Bmtl - Nhel 229 Bami 375 Sgrl 409 Hincll 3905 Scal 3844 Bsl 3787 Bsl 3759 Banll 471 Banll 485 gl 528 Sphi 562 EcoNI 622 Sall - Accl - Hincll 651 Prul 3733 Pstl 3607- Brbi 3602 Pshl 712 Asel 3537 Eagl 909 bell 949 Nrul 972 Bsal 3433 BrDI 3420 PBR322 4,361 bp BAPI 1045 Ahdi 3361 HapMI - Bful 1063 PMI 1315 Buml 1353 PIMI 1364 Styt 1369 Aval - Bsobl 1425 PuMI 1438 Acul 3000 AlwNI 2884 Bell 2777 k 2682 Msel 1444 Bigl 1447 Pyuti 1480 rop Begl 1650 Bspl 1664 Ba 1668 Drll 2575 Peil - Al 2473 rbl 2404 Earl 2351 Xmal 2029 Prull 2064 spol - Sapl 2350 Ndel 2295 BAPI 2291 BomBI 2122 Dedl 2162 BsZ171- Aoct 2244/ Bsal 2225 TthI - PI 2217 Figure 1 If your gene of interest was inserted at the Sphi restriction site of the plasmid illustrated in Figure 1, describe the screening process to select the positive recombinants.

EcoRI 4359 Clal - BspDI 23 Hindi 29 Aatll - Zral 4284 Bki 4209 Borl 4205 EcoRV 185 Sspl 4168 Earl 4155 Acul 4048 Xmnl 3961 Bmtl - Nhel 229 Bami 375 Sgrl 409 Hincll 3905 Scal 3844 Bsl 3787 Bsl 3759 Banll 471 Banll 485 gl 528 Sphi 562 EcoNI 622 Sall - Accl - Hincll 651 Prul 3733 Pstl 3607- Brbi 3602 Pshl 712 Asel 3537 Eagl 909 bell 949 Nrul 972 Bsal 3433 BrDI 3420 PBR322 4,361 bp BAPI 1045 Ahdi 3361 HapMI - Bful 1063 PMI 1315 Buml 1353 PIMI 1364 Styt 1369 Aval - Bsobl 1425 PuMI 1438 Acul 3000 AlwNI 2884 Bell 2777 k 2682 Msel 1444 Bigl 1447 Pyuti 1480 rop Begl 1650 Bspl 1664 Ba 1668 Drll 2575 Peil - Al 2473 rbl 2404 Earl 2351 Xmal 2029 Prull 2064 spol - Sapl 2350 Ndel 2295 BAPI 2291 BomBI 2122 Dedl 2162 BsZ171- Aoct 2244/ Bsal 2225 TthI - PI 2217 Figure 1 If your gene of interest was inserted at the Sphi restriction site of the plasmid illustrated in Figure 1, describe the screening process to select the positive recombinants.

Biology: The Unity and Diversity of Life (MindTap Course List)

15th Edition

ISBN:9781337408332

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter6: Where It Starts-photosynthesis

Section: Chapter Questions

Problem 12SQ: Which of the following substances does not participate in the CalvinBenson cycle? a. ATP b. NADPH c....

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