2nd column: added reagent/s 4th column: observation and interpretation Qualitative Analysis of CarbohydratesCarbohydrates undergo dehydration by Conc. H2SO4 to form furfural(furfuraldehyde) or its derivative. The furfurals further react with a-naphtholpresent in the Molisch's reagent to form a red-violet (purple) colouredproduct that appears as a purple ring at the interface betweenthe acid and the test layers.Select the test:Molisch's testMolisch'ReagentConc.H2SO4ResetGlucoseLactoseSucroseStarch GlucoseMolisch testLactoseSucroseStarch

Carbohydrates are polyhydroxy aldehydes or ketones that are associated with reducing properties.…

Answered: 2nd column: added reagent/s 4th…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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topic: ISOLATION AND CHARACTERIZATION OF CARBOHYDRATES 1. Erythrose is an aldoterose. Describe the result when it is subjected to each of the following tests:a. Molisch’s Testb. Barfoed’s Testc. Benedict’s Testd. Seliwanoff’s Test2. Illustrate the reaction and identify the principle involved in the Nelson test.
Using DEAE-cellulose as ion exchange resin, indicate the starting and ending pH for the narrowest experimental pH range used to separate an amino acid mixture consisting of Gln, Leu and Lys Starting pH: _____ Ending pH: _____
Given ß- Cyclodextrin Briefly explain its expected reaction (based on their structural formula) to the different qualitative tests for carbohydrates which are: (a) Molisch’s test (b)Fehling’s test (c) Bial’s test
Why do ketoses go dehydration reaction much faster than aldoses in Seliwanoff’s test when aldehyde reacts faster than ketoses? Kindly explain in a comprehensive manner.
C. Mucic Acid Test for Galactose and Lactose Galactose, on being oxidized with HNO3 forms mucic acid, an isomer of saccharic acid. Mucic is insoluble andforms characteristic sandy crystals which serve to identify galactose. Examine the crystals under the microscope. Describe the appearance of a few typical crystals.
Chitinase is a protein that breaks down chitin, a primary component of the cell wall in fungi, scales in fish and exoskeletons of arthropods. The activity of chitinase extracted from a plant was shown to be optimum at pH 5. You were tasked to prepare 300 mL of 150 mM buffer solution for further analysis of the extracted chitinase. REAGENTS Ka 2.5M Acetic acid Solid NaOAc•3H2O [136.08g/mol] 1.76 x 10-5 2.5M NH3 Solid NH4Cl [53.49g/mol] 5.6 x 10-10 2.5M Lactic acid Solid sodium lactate [112.06g/mol] 4.0 x 10-5 5 M HCl 5M NaOH Pls show sol'ns 1. Given the following reagents, give the moles of each component (acid & base).2. What are the mass/volume of the components needed to prepare the buffer? 3. What will the pH of the buffer be if 1mL of 5 M NaOH was added?
Qualitative analysis of urine Detection of urea 1. In sodium hypobromite test, why is there a brisk effervescence of nitrogen that apeared in the test tube thus indication the presence of urea in the urine sample? 2. In urease test, why is there a pink or red color product of the solution thus indicating the presence of urea in the urine sample?
give the significance/role/effect of the reagent/condition in the isolation or analysis of a biomolecule. effect on measured purity if glycogen is subjected directly to nelson's assay without prior hydrolysis
Nonspecific elution of affinity bonded macromolecules is used in affinity chromatography explain why?
Qualitative Tests for Lipids Explain why lipids are insoluble in polar solvents. How do oils and fats differ? How does the degree of unsaturation and structure of fats affect its functionality, for example in the case of trans fats? An unknown sample was tested if there is a presence of lipid, after the test it shows that it is positive for translucent test, soluble with chloroform, didn’t produce a pungent odor in acrolein test, didn’t produce red color for Baudouin Test, and violet color disappears in Huble’s Test. WHAT CAN YOU CONCLUDE FROM THESE RESULTS?
whenanalyzing urine from a patient with kidney disease which organic molecules might be detected which biochemical test will be the most appropriate for detectionof those molecues?
Glycine hydrochloride (Cl− H3N+CH2COOH) is a diprotic acid that contains a carboxylic acid group and an ammonium group and is therefore called an amino acid. It is often used in biochemical buffers. Solve, In analogy with Figure , sketch the titration curve of this diprotic acid.

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