Enzyme kinetics
In biochemistry, enzymes are proteins that act as biological catalysts. Catalysis is the addition of a catalyst to a chemical reaction to speed up the pace of the reaction. Catalysis can be categorized as either homogeneous or heterogeneous, depending on whether the catalysts are distributed in the same phase as that of the reactants. Enzymes are an essential part of the cell because, without them, many organic processes would slow down and thus will affect the processes that are important for cell survival and sustenance.
Regulation of Enzymes
A substance that acts as a catalyst to regulate the reaction rate in the living organism's metabolic pathways without itself getting altered is an enzyme. Most of the biological reactions and metabolic pathways in the living systems are carried out by enzymes. They are specific for their works and work in particular conditions. It maintains the best possible rate of reaction in the most stable state. The enzymes have distinct properties as they can proceed with the reaction in any direction, their particular binding sites, pH specificity, temperature specificity required in very few amounts.
Which of the following statements are false?
- Initial velocities of enzyme reactions are best obtained in the absence of product because it simplifies analysis.
- Initial velocities refer to the velocity of the reaction right after it is initiated.
- The velocity of the reaction as a function of measuring time are curved just like an isothermal binding curve because of substrate binding to the enzyme.
- Initial velocities correspond to the pre-steady state condition for free enzyme.
- Initial velocities can sometimes be measured by spectroscopy such as UV/Vis spectroscopy when monitoring the production of NADH from NAD+.
- The velocity of the reaction will eventually go to zero.
- The reaction will reach equilibrium because of the presence of the enzyme.
- It is always better to use substrate rather than product to measure enzyme kinetics.
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