A biochemist wants to determine the effect of an inhibitor on a certain enzyme. Thedata are shown below:[Substrate](mM)Vo (mM/min)(without inhibitor)Vo (mM/min)(with inhibitor)151.110.67301.811.17452.281.56602.621.88903.092.36Using linear regression analysis, determine the values of Vmax and Km of theenzyme in the absence of an inhibitor.y-int[ Select ]slope[ Select]• Vmax (Sclect]mM/minKm[ Sclect)mM Using linear regression analysis, determine the values of Vmax and Km of theenzyme in the presence of an inhibitor.у-int[ Select }%3Dslope{ Select }%3DVmax[ Select ]mM/minKm :[ Select }mM%3DWhat is the type of inhibition involved?[ Sclect ][Select ]Competitive InhibitionNoncompetitive Inhibition

To determine the values of km and Vmax by lineae regression, we need to plot lb plot. LB plot can be…

Answered: A biochemist wants to determine the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Use the relationships revealed by a Lineweaver-Burk plot and the table of enzyme performance to calculate the Vmax and Km of the enzyme with no inhibitor. with inhibitor A, and with inhibitor B. [S] (uM) Vo (umol/min); w/ no inhib. Vo (umol/min); w/ inhib. A Vo (umol/min); w/ inhib. B 10 6.3 5.1 4.0 40 18.4 15.8 11.8 100 29.9 27.0 19.1 150 34.7 32.0 22.2 No inhib. Vmax= Km= Inhib. A Vmax= Km= Inhib. B Vmax= Km=
The concentration of hydrolyzed nitrocefin at each time point for an experiment is given below. Time (min) Concentration (μM) 0.5 6.01 1 11.78 1.5 17.6 2 23.51 2.5 29.58 3 35.31 3.5 39.73 4 44.5 4.5 48.18 5 50.05 5.5 52.72 6 54.01 6.5 55.06 7 55.65 7.5 56.39 8 56.74 8.5 57.49 9 58.03 9.5 58.61 10 58.69 Make a graph that plots the concentration of hydrolyzed nitrocefin (in μM) against time (in seconds) using Excel, R, SPSS or other computable software. Where appropriate, include a trendline that shows the linear range on your graph. Include the equations for the trendlines and the R2 value on the graph. Your graph should also include a title and appropriate titles for the x- and y-axes, with units included where appropriate. To determine the initial velocity of a possible insert in this experiment, you must determine what the linear range is in these data (see note below). Based on your graph, which time points represent a suitable linear…
Why are DG°' values not rigorously applicable to biochemical systems?
The rate of an enzyme-catalysed reaction was measured in the absence or presence of a non-competitive inhibitor. Which of the following Lineweaver-Burk plots would you expect to see? a. (a) b. (b) c. (c) d. (d)
Name the three basic classes of drug targets in medicinal chemistry and indicate, which are the most/least important as targets for biologics? I don't sure if I answer these questions correctly. Hope someone can give me confirmation and explain to me how enzymes will be affected by bioloigcs? My answer so far: The three common classes of drug targets are; receptors, nucleic acid, and enzymes. As a target for biologics, the least important will be a nucleic acid, and the most important will be receptor and second enzymes. Biologic drugs such as monoclonal antibodies will bind to the antigens, which are overexpressed receptor that need to be deactivated. In this case, the monoclonal antibodies will act as an antagonist and block the ligand from binding to the antigens, which are extensively presented in cancer cells. In addition, it can also affect enzymes by.......?
Briefly comment on the differences of using a fixed-time assay versus a kinetic assay to measure enzyme activity. Is it reasonable to assume that the reaction velocity obtained by measuring the amount of product after 30 minutes in a fixed-time assay is directly proportional to absorbance? How could you determine whether this was the case? Word limit 180 words including citation and reference
Topic: Effects of Smoking to Mental Health of Nursing Students Main Claim: Smoking has negative effects on the mental health of nursing students. What are the solution sub-claim/s that you formulated to support your main claim? What is the warrant that connects your solution argument and ground (evidence)? What factors did you consider when identifying possible solution/s to your identified problem? How are you sure that the proposed solution/s will alleviate or help address your identified problem? Has your proposed solution been used to address similar problem? If yes, what has been the outcome of the implementation of such solution?
A TAMU undergraduate biochemistry student is tasked with characterizing a new enzyme. After devising an assay, the student does some initial experiments. When initiating the reaction by adding the enzyme to a solution of substrates (but no products) she finds that at the earliest times the change in the [A] is non-linear and increasing and then becomes linear for awhile and then decreases eventually to zero. Explain what is unusual and what various aspects of the time course mostly likely means.
In an enzyme experiment, when the enzyme is added only to the substrate, the Vmax value is 0.828 and the Km value is 17.76. When the substrate and activator are added to this enzyme, the Vmax is 0.964 and the Km value is 10.6066. When substrate and inhibitor are added to the enzyme, the Vmax value is 0.550 and the Km value is 11.34. Estimate the effect of the substance added. Are these substances suitable activators/inhibitors for the laccase enzyme?
Dr. Vanier’s daughter Lexy thinks that the snacking habit alone does not cause weight loss. She believes that one needs to combine no snacking with the elimination of carbohydrates in their meals. Please explain how Dr. Vanier may test this hypothesis by describing the research design. Specify the independent variable(s), dependent variable(s), and the name of the design. Finally, describe what she needs to find (i.e., what type of effect and what it looks like) in the experiment to support her hypothesis. ( name the IV(s); 1name the DV(s); name the design; then explain the effect that will support the hypothesis)
What are the possible effects and changes on the Michaelis-Menten equation that can result from competitive, noncompetitive, mixed noncompetitive and uncompetitive inhibitors?
The Lineweaver-Buck plot is said to give undue weight to measurements made at low substrate concentrations, when the results are likely to be more accurate?

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