The table below lists experimental conditions that can be applied to a reaction catalyzed by a hypothetical Michaelis- Menten enzyme. For each experimental condition described, complete the table to indicate as precisely as possible the effect on the maximal velocity, Vmax, and Michaelis constant, KM , of the hypothetical enzyme. Experimental condition Vmax Км Twice as much enzyme is used. No change Doubles Half as much enzyme is used. Half as large Half as large A competitive inhibitor is present. Doubles Decreases An uncompetitive inhibitor is present. Increases Increases A pure noncompetitive inhibitor is present. Decreases No change

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